Abstract
In the last years, a rapid development in production, and functionalization of graphene give rise to several products that have shown great potentials in many fields, such as nanoelectronics, energy technology, sensors, and catalysis. In this context we should not forget the biomedical application of graphene that became a new area with outstanding potential. The first study on graphene for biomedical applications has been performed by Dai in 2008 that reported the use of graphene oxide as an efficient nanocarrier for drug delivery. This pioneristic study opened the doors for the use of graphene in widespread biomedical applications such as drug/gene delivery, biological sensing and imaging, antibacterial materials, but also as biocompatible scaffold for cell culture and tissue engineering. The application of graphene-based scaffolds for tissue engineering applications is confirmed by the many exciting and intriguing literature reports over the last few years, that clearly confirm that graphene and its related substrates are excellent platforms for adhesion, proliferation, and differentiation of various cells such as human Mesenchymal stem cells, human neuronal stem cells, and induced pluripotent stem cells. Since most of the papers on this fields are related to in vitro studies, several future in vivo investigations need to be conducted in order to lead to its utilization as implantable tissue engineering material.
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References
Khalili AA, Ahmad MR (2015) A review of cell adhesion studies for biomedical and biological applications. Int J Mol Sci 16(8):18149–18184
Chandra P, Lee SJ (2015) Synthetic extracellular microenvironment for modulating stem cell behaviors. Biomark Insights 10(Suppl 1):105–116
Martino MM, Brkic S, Bovo E, Burger M, Schaefer DJ, Wolff T, Gürke L, Briquez PS, Larsson HM, Gianni-Barrera R, Hubbell JA, Banfi A (2015) Extracellular matrix and growth factor engineering for controlled angiogenesis in regenerative medicine. Front Bioeng Biotechnol 3:45
Knothe Tate ML, Detamore M, Capadona JR, Woolley A, Knothe U (2016) Engineering and commercialization of human-device interfaces, from bone to brain. Biomaterials 95:35–46
Hendow EK, Guhmann P, Wright B, Sofokleous P, Parmar N, Day RM (2016) Biomaterials for hollow organ tissue engineering. Fibrogenesis Tissue Repair 9:3
Bressan E, Ferroni L, Gardin C, Sbricoli L, Gobbato L, Ludovichetti FS, Tocco I, Carraro A, Piattelli A, Zavan B (2014) Graphene based scaffolds effects on stem cells commitment. J Transl Med 12(1):296
Nayak TR, Andersen H, Makam VS, Khaw C, Bae S, Xu X, Ee PL, Ahn JH, Hong BH, Pastorin G, Özyilmaz B (2011) Graphene for controlled and accelerated osteogenic differentiation of human mesenchymal stem cells. ACS Nano 5:4670–4678
Liu Z, Robinson JT, Sun XM et al (2008) PEGylated nanographene oxide for delivery of water-insoluble cancer drugs. J Am Chem Soc 130(33):10876–10877
Shen H, Zhang L, Liu M, Zhang Z (2012) Biomedical applications of graphene. Theranostics 2(3):283–294
Xuan Y, Wu YQ, Shen T et al (2008) Atomic-layer-deposited nanostructures for graphene-based nanoelectronics. Appl Phys Lett 92(1):013101–013103
Hu S, Zeng Y, Yang S, Qin H, Cai H, Wang J (2015) Application of graphene based nanotechnology in stem cells research. J Nanosci Nanotechnol 15(9):6327–6341 (Review)
Zavan B, Vindigni V, Gardin C, D’Avella D, Della Puppa A, Abatangelo G, Cortivo R (2010) Neural potential of adipose stem cells. Discov Med 10(50):37–43 (Review)
Bressan E, Carraro A, Ferroni L, Gardin C, Sbricoli L, Guazzo R, Stellini E, Roman M, Pinton P, Sivolella S, Zavan B (2013) Nanotechnology to drive stem cell commitment. Nanomedicine 8(3):469–486
Hao J, Zhang Y, **g D, Shen Y, Tang G, Huang S, Zhao Z (2015) Mechanobiology of mesenchymal stem cells: perspective into mechanical induction of MSC fate. Acta Biomater 20:1–9
Kfoury Y, Scadden DT (2015) Mesenchymal cell contributions to the stem cell niche. Cell Stem Cell 16(3):239–253
D’souza N, Rossignoli F, Golinelli G, Grisendi G, Spano C, Candini O, Osturu S, Catani F, Paolucci P, Horwitz EM, Dominici M (2015) Mesenchymal stem/stromal cells as a delivery platform in cell and gene therapies. BMC Med 13:186
Schipani E, Kronenberg HM (2008) Adult mesenchymal stem cells. StemBook [Internet]. Harvard Stem Cell Institute, Cambridge
Owen M (1988) Marrow stromal stem cells. J Cell Sci Suppl 10:63–76 (Review)
Caplan AI (2016) MSCs: the sentinel and safe-guards of injury. J Cell Physiol 231(7):1413–1416
González F, Huangfu D (2016) Mechanisms underlying the formation of induced pluripotent stem cells. Wiley Interdiscip Rev Dev Biol 5(1):39–65
Takahashi K, Yamanaka S (2015) A developmental framework for induced pluripotency. Development 142(19):3274–3285
Raab S, Klingenstein M, Liebau S, Linta L (2014) A Comparative view on human somatic cell sources for iPSC generation. Stem Cells Int 2014:768391
Inoue H, Nagata N, Kurokawa H, Yamanaka S (2014) PS cells: a game changer for future medicine. EMBO J 33(5):409–417
Fulka J Jr, Fulka H (2007) Somatic cell nuclear transfer (SCNT) in mammals: the cytoplast and its reprogramming activities. Adv Exp Med Biol 591:93–102 (Review)
Tweedell KS (2008) New paths to pluripotent stem cells. Curr Stem Cell Res Ther 3(3):151–162
Wu M, Chen G, Hu B (2013) Induced pluripotency for translational research. Genom Proteom Bioinform 11(5):288–293
Okano H, Nakamura M, Yoshida K, Okada Y, Tsuji O, Nori S, Ikeda E, Yamanaka S, Miura K (2013) Steps toward safe cell therapy using induced pluripotent stem cells. Circ Res 112(3):523–533
Chari S, Mao S (2016) Timeline: iPSCs—the first decade. Cell 164(3):580
Wan W, Cao L, Kalionis B, **a S, Tai X (2015) Applications of induced pluripotent stem cells in studying the neurodegenerative diseases. Stem Cells Int 2015:382530
Olariu V, Lövkvist C, Sneppen K (2016) Nanog, Oct4 and Tet1 interplay in establishing pluripotency. Sci Rep 6:25438
Takahashi K, Yamanaka S (2016) A decade of transcription factor-mediated reprogramming to pluripotency. Nat Rev Mol Cell Biol 17(3):183–193
Liu Z, Skamagki M, Kim K, Zhao R (2015) Canonical MICRORNA activity facilitates but may be dispensable for transcription factor-mediated reprogramming. Stem Cell Rep 5(6):1119–1127
Porciuncula A, Kumar A, Rodriguez S, Atari M, Araña M, Martin F, Soria B, Prosper F, Verfaillie C, Barajas M (2016) Pancreatic differentiation of Pdx1-GFP reporter mouse induced pluripotent stem cells. Differentiation S0301–4681(16):30015–30019. doi:10.1016/j.diff.2016.04.005
Chanana AM, Rhee JW, Wu JC (2016) Human-induced pluripotent stem cell approaches to model inborn and acquired metabolic heart diseases. Curr Opin Cardiol 31(3):266–274
El Khatib MM, Ohmine S, Jacobus EJ, Tonne JM, Morsy SG, Holditch SJ, Schreiber CA, Uetsuka K, Fusaki N, Wigle DA, Terzic A, Kudva YC, Ikeda Y (2016) Tumor-free transplantation of patient-derived induced pluripotent stem cell progeny for customized islet regeneration. Stem Cells Transl Med 5(5):694–702
Di Foggia V, Makwana P, Ali RR, Sowden JC (2016) Induced pluripotent stem cell therapies for degenerative disease of the outer retina: disease modeling and cell replacement. J Ocul Pharmacol Ther 32(5):240–252. doi:10.1089/jop.2015.0143. Epub 2016 Mar 30
Riera M, Fontrodona L, Albert S, Ramirez DM, Seriola A, Salas A, Muñoz Y, Ramos D, Villegas-Perez MP, Zapata MA, Raya A, Ruberte J, Veiga A, Garcia-Arumi J (2016) Comparative study of human embryonic stem cells (hESC) and human induced pluripotent stem cells (hiPSC) as a treatment for retinal dystrophies. Mol Ther Methods Clin Dev 3:16010
Gabr MM, Zakaria MM, Refaie AF, Khater SM, Ashamallah SA, Ismail AM, El-Badri N, Ghoneim MA (2014) Generation of insulin-producing cells from human bone marrow-derived mesenchymal stem cells: comparison of three differentiation protocols. Biomed Res Int 2014:832736
Reinhard J, Brösicke N, Theocharidis U, Faissner A (2016) The extracellular matrix niche microenvironment of neural and cancer stem cells in the brain. Int J Biochem Cell Biol S1357–2725(16):30107–30108. doi: 10.1016/j.biocel.2016.05.002. [Epub ahead of print]
Belenguer G, Domingo-Muelas A, Ferrón SR, Morante-Redolat JM, Fariñas I (2016) Isolation, culture and analysis of adult subependymal neural stem cells. Differentiation 91(4-5):28–41. doi:10.1016/j.diff.2016.01.005. Epub 2016 Mar 23
Zhao H, Chai Y (2015) Stem cells in teeth and craniofacial bones. J Dent Res 94(11):1495–1501
Aly LA (2015) Stem cells: sources, and regenerative therapies in dental research and practice. World J Stem Cells 7(7):1047–1053
Padial-Molina M, O’Valle F, Lanis A, Mesa F, Dohan Ehrenfest DM, Wang HL, Galindo-Moreno P (2015) Clinical application of mesenchymal stem cells and novel supportive therapies for oral bone regeneration. Biomed Res Int 2015:341327
Tatullo M, Marrelli M, Paduano F (2015) The regenerative medicine in oral and maxillofacial surgery: the most important innovations in the clinical application of mesenchymal stem cells. Int J Med Sci 12(1):72–77
Liu Y, Hu J, Wang S (2014) Mesenchymal stem cell-mediated treatment of oral diseases. Histol Histopathol 29(8):1007–1015
Egusa H, Sonoyama W, Nishimura M, Atsuta I, Akiyama K (2012) Stem cells in dentistry–part II: clinical applications. J Prosthodont Res 56(4):229–248
Dawsonand I, Oreffo ROC (2008) Bridging the regenerationgap:stem cells, biomaterials and clinical translation in bone tissue engineering. Arch Biochem Biophys 473(2):124–131
Morikawa S, Ouchi T, Shibata S, Fujimura T, Kawana H, Okano H, Nakagawa T (2016) Applications of mesenchymal stem cells and neural crest cells in craniofacial skeletal research. Stem Cells Int 2016:2849879
Fisher JN, Peretti GM, Scotti C (2016) Stem cells for bone regeneration: from cell-based therapies to decellularised engineered extracellular matrices. Stem Cells Int 2016:9352598
Yousefi AM, James PF, Akbarzadeh R, Subramanian A, Flavin C, Oudadesse H (2016) Prospect of stem cells in bone tissue engineering: a review. Stem Cells Int 2016:6180487
Liu X, Ma PX (2004) Polymeric scaffolds for bone tissue engineering. Ann Biomed Eng 32(3):477–486
Rezwan K, Chen QZ, Blaker JJ, Boccaccini AR (2006) Biodegradable and bioactive porous polymer/inorganic composite scaffolds for bone tissue engineering. Biomaterials 27(18):3413–3431
Dubey N, Bentini R, Islam I, Cao T, Castro Neto AH, Rosa V (2015) Graphene: a versatile carbon-based material for bone tissue engineering. Stem Cells Int 2015:1–12
Crowder SW, Prasai D, Rath R et al (2013) Three-dimensional graphene foams promote osteogenic differentiation of human mesenchymal stem cells. Nanoscale 5(10):4171–4176
Rosa V, Della Bona A, Cavalcanti BN, Nör JE (2012) Tissue engineering: from research to dental clinics. Dent Mater 28(4):341–348
Qi WY, Yuan W, Yan J, Wang H (2014) Growth and accelerated differentiation of mesenchymal stem cells on graphene oxide/poly-L-lysine composite films. J Mater Chem B 2:5461–5467
Nayak TR, Andersen H, Makam VS et al (2011) Graphene for controlled and accelerated osteogenic differentiation of human mesenchymal stem cells. ACS Nano 5(6):4670–4678
Tang LAL, Lee WC, Shi H et al (2012) Highly wrinkled crosslinked graphene oxide membranes for biological and chargestorage applications. Small 8(3):423–431
** G, Li K (2014) The electrically conductive scaffold as the skeleton of stem cell niche in regenerative medicine. Mater Sci Eng C Mater Biol Appl 45:671–681
Feng L, Wu L, Qu X (2013) New horizons for diagnostics and therapeutic applications of graphene and graphene oxide. Adv Mater 25(2):168–186
Li M, Liu Q, Jia Z et al (2014) Graphene oxide/hydroxyapatite composite coatings fabricated by electrophoretic nanotechnology for biological applications. Carbon 67:185–197
Cheng C, Li D (2013) Solvated graphenes: an emerging class of functional softmaterials. Adv Mater 25(1):13–30
Bernhard JC, Vunjak-Novakovic G (2016) Should we use cells, biomaterials, or tissue engineering for cartilage regeneration? Stem Cell Res Ther 7:56
Hoppe A, Güldal NS, Boccaccini AR (2011) A review of the biological response to ionic dissolution products from bioactive glasses and glass-ceramics. Biomaterials 32(11):2757–2774
Baino F, Vitale-Brovarone C (2011) Three-dimensional glass-derived scaffolds for bone tissue engineering: current trends and forecasts for the future. J Biomed Mater Res A 97(4):514–535
Baino F, Novajra G, Vitale-Brovarone C (2015) Bioceramics and scaffolds: a winning combination for tissue engineering. Front Bioeng Biotechnol 3:202
Jung HS, Lee T, Kwon IK, Kim HS, Hahn SK, Lee CS (2015) Surface modification of multipass caliber-rolled Ti alloy with dexamethasone-loaded graphene for dental applications. ACS Appl Mater Interfaces 7(18):9598–9607
Shi YY, Li M, Liu Q, Jia ZJ, Xu XC, Cheng Y, Zheng YF (2016) Electrophoretic deposition of graphene oxide reinforced chitosan-hydroxyapatite nanocomposite coatings on Ti substrate. J Mater Sci Mater Med 27(3):48
Schroeder KL, Goreham RV, Nann T (2016) Graphene quantum dots for theranostics and bioimaging. Pharm Res 33(10):2337–2357. doi:10.1007/s11095-016-1937-x. Epub 2016 May 20
Hsiao YS, Kuo CW, Chen P (2013) Multifunctional graphene-PEDOT microelectrodes for on chip manipulation of human mesenchymal stem cells. Adv Funct Mater 23(37):4649–4656
Elkhenany H, Amelse L, Lafont A, Bourdo S, Caldwell M, Neilsen N, Dervishi E, Derek O, Biris AS, Anderson D, Dhar M (2015) Graphene supports in vitro proliferation and osteogenic differentiation of goat adult mesenchymal stem cells: potential for bone tissue engineering. J Appl Toxicol 35(4):367–374
Duan S, Yang X, Mei F, Tang Y, Li X, Shi Y, Mao J, Zhang H, Cai Q (2014) Enhanced osteogenic differentiation of mesenchymal stem cells on poly(l-lactide) nanofibrous scaffolds containing carbon nanomaterials. J Biomed Mater Res, Part A 103(4):1424–1435
Kumar S, Raj S, Kolanthai E, Sood AK, Sampath S, Chatterjee K (2015) Chemical functionalization of graphene to augment stem cell osteogenesis and inhibit biofilm formation on polymer composites for orthopedic applications. ACS Appl Mater Interfaces 7(5):3237–3252
Nair M, Nancy D, Krishnan AG, Anjusree GS, Vadukumpully S, Nair SV (2015) Graphene oxide nanoflakes incorporated gelatin-hydroxyapatite scaffolds enhance osteogenic differentiation of human mesenchymal stem cells. Nanotechnology 26(16):161001
Lyu CQ, Lu JY, Cao CH, Luo D, Fu YX, He YS, Zou DR (2015) Induction of osteogenic differentiation of human adipose-derived stem cells by a novel self-supporting graphene hydrogel film and the possible underlying mechanism. ACS Appl Mater Interfaces 7(36):20245–20254
Luo Y, Shen H, Fang Y, Cao Y, Huang J, Zhang M, Dai J, Shi X, Zhang Z (2015) Enhanced proliferation and osteogenic differentiation of mesenchymal stem cells on graphene oxide-incorporated electrospun poly(lactic-co-glycolic acid) nanofibrous mats. ACS Appl Mater Interfaces 7(11):6331–6339
Lee JH, Shin YC, ** OS, Kang SH, Hwang Y-S, Park J-C, Hong SW, Han DW (2015) Reduced graphene oxide-coated hydroxyapatite composites stimulate spontaneous osteogenic differentiation of human mesenchymal stem cells. Nanoscale 7(27):11642–11651
Lee JH, Shin YC, Lee S-M, ** OS, Kang SH, Hong SW, Huh JB, Han D-W (2015) Enhanced osteogenesis by reduced graphene oxide/hydroxyapatite nanocomposites. Sci Rep 5(November):18833
Raucci MR, Giugliano D, Longo A, Zeppetelli S, Carotenuto G, Ambrosio L (2016) Comparative facile methods for preparing graphene oxide-hydroxyapatite for bone tissue engineering. J Tissue Eng Regen Med. doi:10.1002/term.2119
Olivares-Navarrete R, Rodil SE, Hyzy SL, Dunn GR, Almaguer-Flores A, Schwartz Z, Boyan BD (2015) Role of integrin subunits in mesenchymal stem cell differentiation and osteoblast maturation on graphitic carbon-coated microstructured surfaces. Biomaterials 51:69–79
Brun P, Dickinson SC, Zavan B, Cortivo R, Hollander AP, Abatangelo G (2008) Characteristics of repair tissue in second-look and third-look biopsies from patients treated with engineered cartilage: relationship to symptomatology and time after implantation. Arthritis Res Ther 10(6):R132
Huang BJ, Hu JC, Athanasiou KA (2016) Cell-based tissue engineering strategies used in the clinical repair of articular cartilage. Biomaterials 98:1–22
Chen C, Bang S, Cho Y, Lee S, Lee I, Zhang S, Noh I (2012) Research trends in biomimetic medical materials for tissue engineering: 3D bioprinting, surface modification, nano/micro-technology and clinical aspects in tissue engineering of cartilage and bone Curr Opin Neurol 25(5):597–603. doi:10.1097/WCO.0b013e328357f288
Should Bernhard JC, Vunjak-Novakovic G (2016) We use cells, biomaterials, or tissue engineering for cartilage regeneration? Stem Cell Res Ther 7(1):56. doi:10.1186/s13287-016-0314-3
Nazempour A, Van Wie BJ (2016) Chondrocytes, mesenchymal stem cells, and their combination in articular cartilage regenerative medicine. Ann Biomed Eng 44(5):1325–1354
Panadero JA, Lanceros-Mendez S, Ribelles JL (2016) Differentiation of mesenchymal stem cells for cartilage tissue engineering: Individual and synergetic effects of three-dimensional environment and mechanical loading. Acta Biomater 33:1–12
Lee WC, Lim CH, Su KC, Loh KP, Lim CT (2015) Cell-assembled graphene biocomposite for enhanced chondrogenic differentiation. Small 11(8):963–969
Qazi TH, Mooney DJ, Pumberger M, Geissler S, Duda GN (2015) Biomaterials based strategies for skeletal muscle tissue engineering: existing technologies and future trends. Biomaterials 53:502–521
Tedesco FS, Cossu G (2012) Stem cell therapies for muscle disorders. Curr Opin Neurol 25:597–603
Smith BD, Grande DA (2015) The current state of scaffolds for musculoskeletal regenerative applications. Nat Rev Rheumatol 11(4):213–222
Gentile NE, Stearns KM, Brown EH, Rubin JP, Boninger ML, Dearth CL, Ambrosio F, Badylak SF (2014) Targeted rehabilitation after extracellular matrix scaffold transplantation for the treatment of volumetric muscle loss. Am J Phys Med Rehabil 93(11 Suppl 3):S79–S87
Cezara CA, Mooneya DJ (2015) Biomaterial-based delivery for skeletal muscle repair. Adv Drug Deliv Rev 84:188–197
Mertens JP, Sugg KB, Lee JD, Larkin LM (2014) Engineering muscle constructs for the creation of functional engineered musculoskeletal tissue. Regen Med 9(1):89–100
Duffy RM, Feinberg AW (2014) Engineered skeletal muscle tissue for soft robotics: fabrication strategies, current applications, and future challenges. Wiley Interdiscip Rev Nanomed Nanobiotechnol 6(2):178–195
Sicari BM, Dearth CL, Badylak SF (2014) Tissue engineering and regenerative medicine approaches to enhance the functional response to skeletal muscle injury. Anat Rec 297(1):51–64
Davies BM, Morrey ME, Mouthuy PA, Baboldashti NZ, Hakimi O, Snelling S, Price A, Carr A (2013) Repairing damaged tendon and muscle: are mesenchymal stem cells and scaffolds the answer? Regen Med 8(5):613–630
Zhao C, Andersen H, Ozyilmaz B, Ramaprabhu S, Pastorin G, Ho HK (2015) Spontaneous and specific myogenic differentiation of human mesenchymal stem cells on polyethylene glycol-linked multi-walled carbon nanotube films for skeletal muscle engineering. Nanoscale 7(43):18239–18249
Chaudhuri B, Bhadra D, Moroni L, Pramanik K (2015) Myoblast differentiation of human mesenchymal stem cells on graphene oxide and electrospun graphene oxide–polymer composite fibrous meshes: importance of graphene oxide conductivity and dielectric constant on their biocompatibility. Biofabrication 7(1):015009
Sedaghati T, Seifalian AM (2015) Nanotechnology and bio-functionalisation for peripheral nerve regeneration. Neural Regen Res 10(8):1191–1194. doi:10.4103/1673-5374.162678
Matsumoto K, Ohnishi K, Sekine T, Ueda H, Yamamoto Y, Kiyotani T, Nakamura T, Endo K, Shimizu Y (2000) Use of a newly developed artificial nerve conduit to assist peripheral nerve regeneration across a long gap in dogs. ASAIO J 46:415–420
Takahashi K, Yamanaka S (2006) Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. Cell 126(4):663–676
Hood B, Levene HB, Levi AD (2009) Transplantation of autologous Schwann cells for the repair of segmental peripheral nerve defects. Neurosurg Focus 26:E4
Tsintou M, Dalamagkas K, Seifalian AM (2015) Advances in regenerative therapies for spinal cord injury: a biomaterials approach. Neural Regen Res 10:726–742
Ferroni L, Gardin C, Tocco I, Epis R, Casadei A, Vindigni V, Mucci G, Zavan B (2013) Potential for neural differentiation of mesenchymal stem cells. Adv Biochem Eng Biotechnol 129:89–115
Zavan B, Michelotto L, Lancerotto L, Della Puppa A, D’Avella D, Abatangelo G, Vindigni V, Cortivo R (2010) Neural potential of a stem cell population in the adipose and cutaneous tissues. Neurol Res 32(1):47–54
Gardin C, Piattelli A, Zavan B (2016) Graphene in regenerative medicine: focus on stem cells and neuronal differentiation. Trends Biotechnol 34(6):435–437
Gardin C, Vindigni V, Bressan E, Ferroni L, Nalesso E, Puppa AD, D’Avella D, Lops D, Pinton P, Zavan B (2011) Hyaluronan and fibrin biomaterial as scaffolds for neuronal differentiation of adult stem cells derived from adipose tissue and skin. Int J Mol Sci 12(10):6749–6764
Shah S, Yin PT, Uehara TM, Chueng STD, Yang L, Lee KB (2014) Guiding stem cell differentiation into oligodendrocytes using graphene-nanofiber hybrid scaffolds. Adv Mater 26(22):3673–3680
Weaver CL, Cui XT (2015) Directed neural stem cell differentiation with a functionalized graphene oxide nanocomposite. Adv Healthc Mater 4:1408–1416
Guo W, Wang S, Yu X, Qiu J, Li J, Tang W, Li Z, Mou X, Liu H, Wang Z (2016) Construction of a 3D rGO–collagen hybrid scaffold for enhancement of the neural differentiation of mesenchymal stem cells. Nanoscale 8(4):1897–1904
Shevchenko RV, James SL, James SE (2010) A review of tissue-engineered skin bioconstructs available for skin reconstruction. J R Soc Interface 7:229–258
Tonello C, Zavan B, Cortivo R, Brun P, Panfilo S, Abatangelo G (2003) In vitro reconstruction of human dermal equivalent enriched with endothelial cells. Biomaterials 24(7):1205–1211
Papini R (2004) Management of burn injuries of various depths. Br Med J 329:158–160
Pham C, Greenwood J, Cleland H, Woodruff P, Maddern G (2007) Bioengineered skin substitutes for the management of burns: a systematic review. Burns 33:946–957
Mandrycky C, Wang Z, Kim K, Kim DH (2016) 3D bioprinting for engineering complex tissues. Biotechnol Adv 34(4):422–434
Santema TB, Poyck PP, Ubbink DT (2016) Skin grafting and tissue replacement for treating foot ulcers in people with diabetes. Cochrane Database Syst Rev 2:CD011255
Mitsukawa N, Higaki K, Ito N, Muramatsu H, Karube D, Akita S, Kubota Y, Satoh K (2016) Combination treatment of artificial dermis and basic fibroblast growth factor for skin defects: a histopathological examination. Wounds 28(5):158–166
Tonello C, Vindigni V, Zavan B, Abatangelo S, Abatangelo G, Brun P, Cortivo R (2005) In vitro reconstruction of an endothelialized skin substitute provided with a microcapillary network using biopolymer scaffolds. FASEB J 19(11):1546–1548 Epub 2005 Jun 21
Garwood CS, Steinberg JS, Kim PJ (2015) Bioengineered alternative tissues in diabetic wound healing. Clin Podiatr Med Surg 32(1):121–133
Figallo E, Flaibani M, Zavan B, Abatangelo G, Elvassore N (2007) Micropatterned biopolymer 3D scaffold for static and dynamic culture of human fibroblasts. Biotechnol Prog 23(1):210–216
Nyame TT, Chiang HA, Leavitt T, Ozambela M, Orgill DP (2015) Tissue-engineered skin substitutes. Plast Reconstr Surg 136(6):1379–1388
Dąbrowska AK, Rotaru GM, Derler S, Spano F, Camenzind M, Annaheim S, Stämpfli R, Schmid M, Rossi RM (2016) Materials used to simulate physical properties of human skin. Skin Res Technol 22(1):3–14
Rowan MP, Cancio LC, Elster EA, Burmeister DM, Rose LF, Natesan S, Chan RK, Christy RJ, Chung KK (2015) Burn wound healing and treatment: review and advancements. Crit Care 12(19):243
Wang HY, Zhang YQ (2015) Processing silk hydrogel and its applications in biomedical materials. Biotechnol Prog 31(3):630–640
Sun BK, Siprashvili Z, Khavari PA (2014) Advances in skin grafting and treatment of cutaneous wounds. Science 346(6212):941–945
Li Z, Wang H, Yang B, Sun Y, Huo R (2015) Three-dimensional graphene foams loaded with bone marrow derived mesenchymal stem cells promote skin wound healing with reduced scarring. Mater Sci Eng, C 57:181–188
Farouz Y, Chen Y, Terzic A, Menasché P (2015) Concise review: growing hearts in the right place: on the design of biomimetic materials for cardiac stem cell differentiation. Stem Cells 33(4):1021–1035
Sun X, Altalhi W, Nunes SS (2016) Vascularization strategies of engineered tissues and their application in cardiac regeneration. Adv Drug Deliv Rev 96:183–194
Alrefai MT, Murali D, Paul A, Ridwan KM, Connell JM, Shum-Tim D (2015) Cardiac tissue engineering and regeneration using cell-based therapy. Stem Cells Cloning 8:81–101
Huyer LD, Montgomery M, Zhao Y, **ao Y, Conant G, Korolj A, Radisic M (2015) Biomaterial based cardiac tissue engineering and its applications. Biomed Mater 10(3):034004
Lee T-J, Park S, Bhang SH, Yoon J-K, Jo I, Jeong G-J, Hong BH, Kim B-S (2014) Graphene enhances the cardiomyogenic differentiation of human embryonic stem cells. Biochem Biophys Res Commun 452(1):174–180
Ahmed M, Yildirimer L, Khademhosseini A, Seifalian AM (2012) Nanostructured materials for cardiovascular tissue engineering. J Nanosci Nanotechnol 12(6):4775–4785
Martinelli V, Cellot G, Fabbro A, Bosi S, Mestroni L, Ballerini L (2013) Improving cardiac myocytes performance by carbon nanotubes platforms. Front Physiol 4:239
Sreejit P, Verma RS (2013) Natural ECM as biomaterial for scaffold based cardiac regeneration using adult bone marrow derived stem cells. Stem Cell Rev 9(2):158–171
Zwi-Dantsis L, Gepstein L (2012) Induced pluripotent stem cells for cardiac repair. Cell Mol Life Sci 69(19):3285–3299
Kim T, Kahng YH, Lee T, Lee K, do Kim H (2013) Graphene films show stable cell attachment and biocompatibility with electrogenic primary cardiac cells. Mol Cells 36(6):577–582
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Buggio, M. et al. (2016). Stem Cells Commitment on Graphene-Based Scaffolds. In: Gonçalves , G., Marques, P., Vila, M. (eds) Graphene-based Materials in Health and Environment. Carbon Nanostructures. Springer, Cham. https://doi.org/10.1007/978-3-319-45639-3_4
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