Abstract
Microdialysis is an in vivo neurochemical monitoring technique that measures changes in the extracellular compartment of selected brain regions. These changes are monitored through a microdialysis probe implanted into the brain tissue using standard stereotaxic techniques. The microdialysis probe consists of a semipermeable dialysis membrane that surrounds two silica capillary tubes that allow fluids flow into and out of the part of the probe containing the membrane. Dialysate samples contain detectable amounts of different substances including endogenous monoamine neurotransmitters. Microdialysis perfusates are applied to a chromatography column with electrochemical detection that separates the different monoamines according to their size, charge, and lipophilicity. In this chapter, we describe in detail the microdialysis methodology currently used in our laboratory to measure in vivo extracellular levels of monoamines in different brain regions.
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Acknowledgments
This work was supported by the Basque Government (IT616-13 and S-PE12UN033), the University of the Basque Country (UFI 11/35), Ministry of Economy and Competitiveness (SAF2009-08460; SAF2013-48586-R), and the Instituto de Salud Carlos III, Centro de Investigación Biomédica en Red de Salud Mental, CIBERSAM, Spain.
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Ortega, J.E., Meana, J.J., Callado, L.F. (2016). In Vivo Brain Microdialysis of Monoamines. In: Luján, R., Ciruela, F. (eds) Receptor and Ion Channel Detection in the Brain. Neuromethods, vol 110. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3064-7_25
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DOI: https://doi.org/10.1007/978-1-4939-3064-7_25
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-3063-0
Online ISBN: 978-1-4939-3064-7
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