Abstract
Microdialysis is an in vivo neurochemical monitoring technique that measures changes in the extracellular compartment of selected brain regions. These changes are monitored through a microdialysis probe implanted into the brain tissue using standard stereotaxic techniques. The microdialysis probe consists of a semipermeable dialysis membrane that surrounds two silica capillary tubes that allow fluids flow into and out of the part of the probe containing the membrane. Dialysate samples contain detectable amounts of different substances including endogenous monoamine neurotransmitters. Microdialysis perfusates are applied to a chromatography column with electrochemical detection that separates the different monoamines according to their size, charge, and lipophilicity. In this chapter, we describe in detail the microdialysis methodology currently used in our laboratory to measure in vivo extracellular concentrations of monoamines in different brain regions.
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References
Gómez FM, Ortega JE, Horrillo I, Meana JJ (2010) Relationship between mon-functional masticatory activity and central dopamine in stressed rats. J Oral Rehabil 37:827–833
Muller M (2013) Microdialysis in drug development. Springer, New York, NY
Ortega JE, Fernández-Pastor B, Callado LF, Meana JJ (2010) In vivo potentiation of reboxetine and citalopram effect on extracellular noradrenaline in rat brain by the α2-adrenoceptor antagonist RS79948. Eur Neuropsychopharmacol 20:813–822
Ortega JE, Gonzalez-Lira V, Horrillo I, Herrera-Marschitz M, Callado LF, Meana JJ (2013) Additive effect of rimonabant and citalopram on extracellular serotonin levels monitored with in vivo microdialysis in rat brain. Eur J Pharmacol 709:13–19
Paxinos G, Watson C (1986) The rat brain in stereotaxic coordinates, 2nd edn. Academic Press, Orlando, USA
Perez-Palomar B, Mollinedo-Gajate I, Berrocoso E, Meana JJ, Ortega JE (2018) Serotonin 5-HT3 receptor antagonism potentiates the antidepressant activity of citalopram. Neuropharmacology 133:491–502
Franklin K, Paxinos G (1997) The mouse brain in stereotaxic coordinates. Academic Press, San Diego
Wahlsten D, Hudspeth WJ, Bernhardt K (1975) Implications of genetic variation in mouse brain structure for electrode placement by stereotaxic surgery. J Comp Neurol 162:519–531
Westerink BHC, Cremers TIFH (2007) Handbook of microdialysis. Methods, applications and clinical aspects. Elsevier, Amsterdam
Zapata A, Chefer VI, Shippenberg TS (2009) Microdialysis in rodents. Curr Protoc Neurosci Chapter 7:Unit 7.2
Acknowledgments
This work was supported by the Basque Government (IT1211-19), Ministry of Science and Innovation, ERDF (SAF-2013-48586-R and SAF-2017-88126-R), and the Instituto de Salud Carlos III, Centro de Investigación Biomédica en Red de Salud Mental, CIBERSAM, Spain.
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Ortega, J.E., Perez-Palomar, B., Meana, J.J., Callado, L.F. (2021). In Vivo Brain Microdialysis of Monoamines. In: Lujan, R., Ciruela, F. (eds) Receptor and Ion Channel Detection in the Brain. Neuromethods, vol 169. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1522-5_29
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DOI: https://doi.org/10.1007/978-1-0716-1522-5_29
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