Abstract
Respiratory measurement in plants is one of the commonly used techniques to assess metabolic activity and in vivo redox state of plant mitochondria. However, respiration rate monitoring of Arabidopsis (Arabidopsis thaliana) remains a challenge for researchers due to the small size of its organs. In this chapter we introduce adaptations to micro-respiratory technologies to study three tissues of special interest to plant biologists: leaf sections, root tips, and seeds in this model plant species. This assay opens up new possibilities to screen and study mutants and to identify differences in ecotypes or populations of plants.
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Acknowledgments
Development of these protocols was funded by the Australian Research Council through the ARC Centre of Excellence in Plant Energy Biology (CE140100008). Y.S.S. is supported by a PhD scholarship from Malaysian Agricultural Research and Development Institute (MARDI). A.H.M. is supported by an ARC Future Fellowship (FT110100242) and E.S. by an ARC Postdoctoral Fellowship (DP110104865).
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Sew, Y.S., Millar, A.H., Stroeher, E. (2015). Micro-Respiratory Measurements in Plants. In: Whelan, J., Murcha, M. (eds) Plant Mitochondria. Methods in Molecular Biology, vol 1305. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2639-8_13
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DOI: https://doi.org/10.1007/978-1-4939-2639-8_13
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