Abstract
Respiratory rate measurements are crucial assays to understand mitochondrial biochemistry as well as metabolic regulation within tissues. Several technologies currently exist that can measure plant respiratory oxygen consumption or carbon dioxide evolution rates over short durations by either isolated mitochondria or plant tissues. Here we describe recently developed alternative methods for measuring tissue oxygen consumption rates (OCRs) using systems reliant on oxygen sensitive fluorophores. The methods described have distinct experimental advantages: they can allow high-throughput and long-duration measurements; and they are particularly suited to investigating the metabolic regulation of respiration by comparing OCRs among treatments or genotypes.
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References
Millar AH, Whelan J, Soole KL, Day DA (2011) Organization and regulation of mitochondrial respiration in plants. Annu Rev Plant Biol 62:79–104
Tcherkez G, Gauthier P, Buckley TN, Busch FA, Barbour MM, Bruhn D, Heskel MA, Gong XY, Crous KY, Griffin K, Way D, Turnbull M, Adams MA, Atkin OK, Farquhar GD, Cornic G (2017) Leaf day respiration: low CO2 flux but high significance for metabolism and carbon balance. New Phytol 216(4):986–1001
O'Leary BM, Asao S, Millar AH, Atkin OK (2019) Core principles which explain variation in respiration across biological scales. New Phytol 222(2):670–686
Atkin OK, Tjoelker MG (2003) Thermal acclimation and the dynamic response of plant respiration to temperature. Trends Plant Sci 8(7):343–351
Atkin OK, Macherel D (2009) The crucial role of plant mitochondria in orchestrating drought tolerance. Ann Bot 103(4):581–597
O'Leary BM, Oh GGK, Lee CP, Millar AH (2020) Metabolite regulatory interactions control plant respiratory metabolism via target of rapamycin (TOR) kinase activation. Plant Cell 32(3):666–682
Scafaro AP, Negrini ACA, O'Leary B, Rashid FAA, Hayes L, Fan Y, Zhang Y, Chochois V, Badger MR, Millar AH, Atkin OK (2017) The combination of gas-phase fluorophore technology and automation to enable high-throughput analysis of plant respiration. Plant Methods 13:16
O'Leary BM, Lee CP, Atkin OK, Cheng R, Brown TB, Millar AH (2017) Variation in leaf respiration rates at night correlates with carbohydrate and amino acid supply. Plant Physiol 174(4):2261–2273
Van Belle G (2011) Statistical rules of thumb, vol 699. Wiley, New York
Florez-Sarasa I, Araujo WL, Wallström SV, Rasmusson AG, Fernie AR, Ribas-Carbo M (2012) Light-responsive metabolite and transcript levels are maintained following a dark-adaptation period in leaves of Arabidopsis thaliana. New Phytol 195(1):136–148
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O’Leary, B.M., Oh, G.G.K., Millar, A.H. (2022). High-Throughput Oxygen Consumption Measurements in Leaf Tissue Using Oxygen Sensitive Fluorophores . In: Van Aken, O., Rasmusson, A.G. (eds) Plant Mitochondria. Methods in Molecular Biology, vol 2363. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1653-6_6
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DOI: https://doi.org/10.1007/978-1-0716-1653-6_6
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Publisher Name: Humana, New York, NY
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Online ISBN: 978-1-0716-1653-6
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