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Immunocytochemistry of Acutely Isolated Adrenal Medullary Chromaffin Cells
Immunocytochemistry enables the detection and localization of proteins in cells that are acutely dissociated or in culture. There are advantages and...
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Pineal Cells Dissociation and Culture: Isolated Pinealocytes, Isolated Astrocytes, and Co-culture
Mammalian pineal glands are composed mostly of pinealocytes, which are the melatonin secretory cells, and also importantly of glial cells in special...
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Development of KoRV-pseudotyped lentiviral vectors for efficient gene transfer into freshly isolated immune cells
Allogeneic cell therapies, such as those involving macrophages or Natural Killer (NK) cells, are of increasing interest for cancer immunotherapy....
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Metabolic Labeling of Mitochondrial Translation Products in Whole Cells and Isolated Organelles
Mitochondria retain their own genome and translational apparatus that is highly specialized in the synthesis of a handful of proteins, essential...
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Cryoresistance of Cells Isolated from Human Subcutaneous Adipose Tissue with Phenotype Similar to That of Mesenchymal Stem Cells
AbstractThe idea of treating various diseases in human beings and animals with low temperatures has a great deal of support in medicine, including...
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Adenosine metabolism by mesenchymal stromal cells isolated from different human tissues
Mesenchymal stromal cells (MSCs) have unique biological properties and play important functions, which make them attractive tools for cell-based...
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The therapeutic effects of exosomes the first time isolated from pancreatic islet-derived progenitor cells in the treatment of pancreatic cancer
Insulinoma is an excessive insulin-released beta cell tumor. Pancreas cancer is one of the deadliest malignant neoplasms. Exosomes are secreted cell...
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Assay for Transposase-Accessible Chromatin Using Sequencing of Freshly Isolated Muscle Stem Cells
Actively transcribed genes harbor cis-regulatory modules with comparatively low nucleosome occupancy and few high-order structures (=“open...
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Greatly isolated heterogeneous circulating tumor cells using hybrid engineered cell membrane-camouflaged magnetic nanoparticles
BackgroundCirculating tumor cells (CTCs) are considered as a useful biomarker for early cancer diagnosis, which play a crucial role in metastatic...
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Follicular reconstruction and neo-oogenesis in xenotransplantation of human ovarian isolated cells derived from chemotherapy-induced POF patients
BackgroundDevelo** new strategies to restore fertility in patients with chemotherapy-induced Premature Ovarian Failure (Chemo-POF) is important. We...
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Morphology, chemical composition and thermal stability of bamboo parenchyma cells and fibers isolated by different methods
The effects of different isolation methods on the separated bamboo parenchyma cells and fibers were investigated in terms of the microstructure,...
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The Derivation and Characterization of Different Populations of Mesenchymal Stem Cells Isolated from Human Embryonic Stem Cell Line—SC7
AbstractA comparative analysis of three independent cell populations isolated from SC7 human embryonic stem cells was carried out. Cell properties...
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Human post-implantation blastocyst-like characteristics of Muse cells isolated from human umbilical cord
Muse cells, identified as cells positive for the pluripotent surface marker SSEA-3, are pluripotent-like endogenous stem cells located in the bone...
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The pro-apoptotic activity of sinueracasbanone D isolated from Sinularia Leptoclados in hepatocellular carcinoma cells
The soft-bodied corals of the genera Sarcophyton and Sinularia (Alcyoniidae) are known as a warehouse of casbane and cembranoid diterpenoids with...
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Cordialin A isolated from Varronia curassavica Jacq. loaded in nanoemulsion as potential cytotoxic agent on human cervical tumor cells
Cervical cancer is the fourth most common cancer in women worldwide. Most cases of cervical cancer are caused by the human papillomavirus....
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Intrathecal CAR-NK cells infusion for isolated CNS relapse after allogeneic stem cell transplantation: case report
A 24-year-old man with central nervous system (CNS) involvement of T-cell lineage acute lymphoblastic leukemia received sibling allogeneic stem cell...
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New biflavonoids isolated from Xylia kerrii leaves extract with selective cytotoxicity against MH7A human rheumatoid arthritis synovial fibroblast cells
Three new biflavonoids ( 1 – 3 ) and two known flavonoids ( 4 , 5 ) were isolated from Xylia kerrii collected in Thailand. Compounds 1 – 5 showed selective...
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Acetylmelodorinol isolated from Sphaerocoryne affinis seeds inhibits cell proliferation and activates apoptosis on HeLa cells
BackgroundCervical cancer is a major global health concern with a high prevalence in low- and middle-income countries. Natural products, particularly...
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High-glutathione mesenchymal stem cells isolated using the FreSHtracer probe enhance cartilage regeneration in a rabbit chondral defect model
BackgroundMesenchymal stem cells (MSCs) are a promising cell source for cartilage regeneration. However, the function of MSC can vary according to...
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Understanding the Angiogenic Characteristics of Clinical-Grade Mesenchymal Stromal Cells Isolated from Human Umbilical Cord
Addressing the challenges in managing ischemic tissue repair and remodelling remains a prominent clinical concern. Current research is heavily...
