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DiCleave: a deep learning model for predicting human Dicer cleavage sites
BackgroundMicroRNAs (miRNAs) are a class of non-coding RNAs that play a pivotal role as gene expression regulators. These miRNAs are typically...
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Secondary structure RNA elements control the cleavage activity of DICER
The accurate and efficient cleavage of shRNAs and pre-miRNAs by DICER is crucial for their gene-silencing activity. Here, we conduct high-throughput...
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Sequence determinant of small RNA production by DICER
RNA silencing relies on specific and efficient processing of double-stranded RNA by Dicer, which yields microRNAs (miRNAs) and small interfering RNAs...
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Substrate promiscuity of Dicer toward precursors of the let-7 family and their 3′-end modifications
The human let-7 miRNA family consists of thirteen members that play critical roles in many biological processes, including development timing and...
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RNA structure probing reveals the structural basis of Dicer binding and cleavage
It is known that an RNA’s structure determines its biological function, yet current RNA structure probing methods only capture partial structure...
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Structure of the human DICER–pre-miRNA complex in a dicing state
Dicer has a key role in small RNA biogenesis, processing double-stranded RNAs (dsRNAs)
1 ,2 . Human DICER (hDICER, also known as DICER1) is specialized...
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Caspase-mediated cleavage of miRNA processing proteins Drosha, DGCR8, Dicer, and TRBP2 in heat-shocked cells and its inhibition by HSP70 overexpression
Cells respond to stress through adaptive mechanisms that limit cellular damage and prevent cell death. MicroRNAs act as regulators of stress...
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ReCGBM: a gradient boosting-based method for predicting human dicer cleavage sites
BackgroundHuman dicer is an enzyme that cleaves pre-miRNAs into miRNAs. Several models have been developed to predict human dicer cleavage sites,...
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Single-molecule analysis of processive double-stranded RNA cleavage by Drosophila Dicer-2
Drosophila Dicer-2 (Dcr-2) produces small interfering RNAs from long double-stranded RNAs (dsRNAs), playing an essential role in antiviral RNA...
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Structure of the Dicer-2–R2D2 heterodimer bound to a small RNA duplex
In flies, Argonaute2 (Ago2) and small interfering RNA (siRNA) form an RNA-induced silencing complex to repress viral transcripts
1 . The RNase III...
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DICER: structure, function, and regulation
Regulation of gene expression by small non-coding RNAs, such as miRNAs and siRNAs, is an inherent part of complex biological processes that allow...
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PTBP1 protects Y RNA from cleavage leading to its apoptosis-specific degradation
Some RNAs such as 28S rRNA, U1 small nuclear RNA (snRNA), and Y RNAs are known to be cleaved during apoptosis. The underlying mechanism, functions,...
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Structural insights into dsRNA processing by Drosophila Dicer-2–Loqs-PD
Small interfering RNAs (siRNAs) are the key components for RNA interference (RNAi), a conserved RNA-silencing mechanism in many eukaryotes
1 ,2 . In Drosophila...
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Pterostilbene–isothiocyanate reduces miR-21 level by impeding Dicer-mediated processing of pre-miR-21 in 5-fluorouracil and tamoxifen-resistant human breast cancer cell lines
Converging evidences identifies that microRNA-21 (miR-21) is responsible for drug resistance in breast cancer. This study aims to evaluate the...
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RNA and DNA G-quadruplexes bind to human dicer and inhibit its activity
Guanine (G)-rich single-stranded nucleic acids can adopt G-quadruplex structures. Accumulating evidence indicates that G-quadruplexes serve important...
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Comparative phylogeny and evolutionary analysis of Dicer-like protein family in two plant monophyletic lineages
BackgroundSmall RNAs (sRNAs) that do not get untranslated into proteins exhibit a pivotal role in the expression regulation of their cognate gene(s)...
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The conserved single-cleavage mechanism of animal DROSHA enzymes
RNase III enzymes typically cleave both strands of double-stranded RNAs (dsRNAs). We recently discovered that a human RNase III, DROSHA, exhibits a...
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Template and target-site recognition by human LINE-1 in retrotransposition
The long interspersed element-1 (LINE-1, hereafter L1) retrotransposon has generated nearly one-third of the human genome and serves as an active...
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Selective cleavage of ncRNA and antiviral activity by RNase2/EDN in THP1-induced macrophages
RNase2 is the member of the RNaseA family most abundant in macrophages. Here, we knocked out RNase2 in THP-1 cells and analysed the response to...
