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Intravitreal Delivery of PEGylated-ECO Plasmid DNA Nanoparticles for Gene Therapy of Stargardt Disease
ObjectiveCurrent gene therapy of inherited retinal diseases is achieved mainly by subretinal injection, which is invasive with severe adverse...
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Plasmid Vaccines and Therapeutics: From Design to Applications
In the late 1980s, Vical and collaborators discovered that the injection into tissues of unformulated plasmid encoding various proteins resulted in...
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Investigating Efficacy of Three DNA-Aptamers in Targeted Plasmid Delivery to Human Prostate Cancer Cell Lines
Selection of targeted and efficient carriers to deliver drugs and genes to cells and tissues is still a major challenge and to overcome this...
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Realizing time-staggered expression of nucleic acid-encoded proteins by co-delivery of messenger RNA and plasmid DNA on a single nanocarrier
Co-delivery of different protein-encoding polynucleotide species with varying expression kinetics of their therapeutic product will become a...
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Transfection with Plasmid-Encoding lncRNA-SLERCC nanoparticle-mediated delivery suppressed tumor progression in renal cell carcinoma
BackgroundThe accumulating evidence confirms that long non-coding RNAs (lncRNAs) play a critical regulatory role in the progression of renal cell...
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Nonviral Delivery of Cancer Genetic Vaccines
The potential use of genetic vaccines to address numerous diseases including cancer is promising, but currently unrealized. Here, we review...
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CRISPR/Cas9 Plasmid Delivery Through the CPP: PepFect14
Gene editing is increasing its popularity day by day especially as an essential tool for the research. It is based on two recombination mechanisms in...
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Attenuation of osteoarthritis progression via locoregional delivery of Klotho-expressing plasmid DNA and Tanshinon IIA through a stem cell-homing hydrogel
BackgroundOsteoarthritis (OA) is an aging-related degenerative joint disorder marked by joint discomfort and rigidity. Senescent chondrocytes release...
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Modification of the tumor microenvironment enhances immunity with plasmid gene therapy
Local intratumor delivery with electroporation of low levels of plasmids encoding molecules, induces an antitumor effect without causing systemic...
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Effect of Different Karyophilic Peptides on Physical Characteristics and In Vitro Transfection Efficiency of Chitosan-Plasmid Nanoparticles as Nonviral Gene Delivery Systems
A strategy to increase the transfection efficiency of chitosan-based nanoparticles for gene therapy is by adding nuclear localization signals through...
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Apoptosis-enhanced ferroptosis therapy of pancreatic carcinoma through PAMAM dendrimer-iron(III) complex-based plasmid delivery
The development of promising strategies to improve the treatment efficacy of pancreatic carcinoma still remains to be a challenging task. We report...
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Influence of Liposome Composition on Plasmid DNA Delivery to Eukaryotic Cells
AbstractGene therapy is a promising method for the treatment of various diseases by introducing therapeutic nucleic acids, for the delivery of which...
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Citric acid/β-alanine carbon dots as a novel tool for delivery of plasmid DNA into E. coli cells
Successful delivery of plasmid DNA into the microbial cells is fundamental in recombinant DNA technology. Natural bacterial transformation is limited...
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Microfluidic Approaches for Gene Delivery and Therapy
Gene editing techniques such as Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR/Cas9) have been developed to target a gene into...
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Producing Plasmid DNA Template for Clinical Grade RNA Vaccine Manufacture
A plasmid production process has been established to manufacture plasmid DNA at a large scale in High-Quality grade. This is used as a starting...
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Simple Magnetic Nanoparticle-Based System for Gene Delivery
Due to their unique properties such as superparamagnetism, biocompatibility and bio-friendly, magnetic nanoparticles (MNPs) have been successfully...
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Tissue Analysis of Lung-Targeted Delivery of siRNA and Plasmid DNA
Development of nucleic acid delivery systems requires the use of adequate tissue analysis methods, especially when aiming tissue-targeted drug...
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Kluyveromyces lactis zymocin and other plasmid-encoded yeast killer toxins
Supported by an endogenous killer plasmid DNA system that codes for an anti-proliferative zymocin toxin, the dairy yeast Kluyveromyces lactis has...
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Molecular dynamics simulation of complexation between plasmid DNA and cationic peptides
The elucidation of the process by which cationic peptides condense plasmid DNA (pDNA) is important for unraveling the mechanism of peptide/pDNA...
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Establishment of a hydrodynamic delivery system in ducks
Chronic hepatitis B virus (HBV) poses a significant global health challenge as it can lead to acute or chronic liver disease and hepatocellular...
