Abstract
Development of nucleic acid delivery systems requires the use of adequate tissue analysis methods, especially when aiming tissue-targeted drug delivery. In this chapter, a protocol is presented for analyzing a reporter signal from the lung tissue. Because lung is an important target tissue from the clinical point of view, yet represents a challenge from the histological point of view, this protocol can be used in any lung-targeting drug delivery project.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Similar content being viewed by others
Reference
Kurrikoff K, Freimann K, Veiman KL et al (2019) Effective lung-targeted RNAi in mice with peptide-based delivery of nucleic acid. Sci Rep 9:19926
Acknowledgments
The authors are supported by the EU project 2014-2020.4.01.15-0013, but this did not bias the current work in any way.
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2022 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature
About this protocol
Cite this protocol
Kurrikoff, K., Vunk, B., Langel, Ü. (2022). Tissue Analysis of Lung-Targeted Delivery of siRNA and Plasmid DNA. In: Langel, Ü. (eds) Cell Penetrating Peptides. Methods in Molecular Biology, vol 2383. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1752-6_34
Download citation
DOI: https://doi.org/10.1007/978-1-0716-1752-6_34
Published:
Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-1751-9
Online ISBN: 978-1-0716-1752-6
eBook Packages: Springer Protocols