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Culture of Continuous Cell Lines
This chapter describes the culture of various continuous cell lines. Continuous cell lines are generated either due to specific genetic or epigenetic...
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Cell Lines for Cultivated Meat Production
Cellular agriculture is an emerging field that aims to provide a more sustainable alternative for the production of animal-derived products, without...
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The Epiblast and Pluripotent Stem Cell Lines
All somatic cells develop from the epiblast, which occupies the upper layer of two-layered embryos and in most mammals is formed after the...
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Single cell multi-omics reveal intra-cell-line heterogeneity across human cancer cell lines
Human cancer cell lines have long served as tools for cancer research and drug discovery, but the presence and the source of intra-cell-line...
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Working with Immortalized Hepatic Stellate Cell Lines
Hepatic stellate cells (HSCs) are the major cellular source of extracellular matrix production in the liver. Therefore, this cell population has...
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Generation of Monoclonal Chinese Hamster Ovary Cell Lines Using DISPENCELL-S3
Single-cell isolation is a key step in the manufacturing of therapeutic proteins, which relies on the development of monoclonal cell lines. It...
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Culturing Suspension Cancer Cell Lines
Suspension cell lines grow free-floating in the cell culture media without any attachment to the culture plate/vessel. Suspension cells typically...
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Resuscitation of Frozen Cell Lines
Cell freezing and thawing have long been a standard procedure in cell biology. The procedure, also known as cryonics, has effectively stopped the...
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Cryopreservation of Cell Lines
Cryopreservation employs amalgamated applications of low temperatures in preserving structural composition along with functional integrity of animal...
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Multiplexed single-cell transcriptome analysis reveals molecular characteristics of monkey pluripotent stem cell lines
Efforts have been made to establish various human pluripotent stem cell lines. However, such methods have not yet been duplicated in non-human...
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Develo** Wolbachia-Infected Cell Lines from Insects
Wolbachia, an intracellular bacterium of arthropods, is an ideal candidate for use in the biological control of insect pests. The inability of...
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Cell morphology best predicts tumorigenicity and metastasis in vivo across multiple TNBC cell lines of different metastatic potential
BackgroundMetastasis is the leading cause of death in breast cancer patients. For metastasis to occur, tumor cells must invade locally, intravasate,...
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Knockout Transporter Cell Lines to Assess Substrate Potential Towards Efflux Transporters
P-glycoprotein (P-gp), breast cancer resistance protein (BCRP) and multidrug resistance transporter 2 (MRP2) are efflux transporters involved in the...
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CRISPR-Cas9 screens reveal common essential miRNAs in human cancer cell lines
BackgroundGenome-wide functional screening using the CRISPR-Cas9 system is a powerful tool to uncover tumor-specific and common genetic dependencies...
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Multi-omics data of gastric cancer cell lines
ObjectivesGastric cancer (GC) is the fourth most common cancer worldwide, with the highest incidence and mortality regardless of sex. Despite...
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CRISPR-Mediated Genome Engineering in Cell Lines
A specific targeting nuclease is a powerful tool for mediating genome alternative expression with high precision. The RNA sequence-guided Cas9...
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Genetic Modification of Tumor-Infiltrating Lymphocytes, Peripheral T Cells, and T-Cell Model Cell Lines
Genetic modification of tumor-infiltrating lymphocytes (TILs) or circulating T cells has become an important avenue in cancer therapy. Here we...
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Cell Cycle–Specific Protein Phosphatase 1 (PP1) Substrates Identification Using Genetically Modified Cell Lines
The identification of protein phosphatase 1 (PP1) holoenzyme substrates has proven to be a challenging task. PP1 can form different holoenzyme...
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Establishment and characterization of two novel patient-derived cell lines from giant cell tumor of bone
Giant cell tumor of bone (GCTB) is a rare bone tumor with osteolytic features, composed of stromal cells with a monotonous appearance, macrophages,...
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Characterisation of cell lines derived from prostate cancer patients with localised disease
BackgroundProstate cancer is a broad-spectrum disease, spanning from indolent to a highly aggressive lethal malignancy. Prostate cancer cell lines...
