Abstract
Cell freezing and thawing have long been a standard procedure in cell biology. The procedure, also known as cryonics, has effectively stopped the cellular clock, thereby adds tremendous repeatability to studies performed at different times by allowing extremely similar samples of cells to be utilised weeks, months or even ages apart. It also saves time, resources and money. The primary issue during the entire freezing–storage–thawing process is to obtain higher cell viability and, perhaps more crucially, predictable and consistent cell behaviour following cryopreservation. Many various rules and regulations have been evolved over time to accomplish this. The development of intracellular ice is the primary cause of cell death during cryopreservation.
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Agarwal, V., Singh, M. (2023). Resuscitation of Frozen Cell Lines. In: Animal Cell Culture: Principles and Practice. Techniques in Life Science and Biomedicine for the Non-Expert. Springer, Cham. https://doi.org/10.1007/978-3-031-19485-6_11
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DOI: https://doi.org/10.1007/978-3-031-19485-6_11
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