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In-vitro generation of follicle-like structures from human germ cell-like cells derived from theca stem cell combined with ovarian somatic cells
BackgroundThe objective of this study was to induce the differentiation of human theca stem cells (hTSCs) into germ cell-like cells (hGCLCs) and...
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Humidified atmosphere in a time-lapse embryo culture system does not improve ongoing pregnancy rate: a retrospective propensity score model study derived from 496 first ICSI cycles
PurposeTo investigate whether high relative humidity conditions (HC), when using a time-lapse system (TLS) with sequential culture media, are...
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The effect of epididymosomes on the development of frozen-thawed mouse spermatogonial stem cells after culture in a decellularized testicular scaffold and transplantation into azoospermic mice
PurposeThis study examined SSC proliferation on an epididymosome-enriched decellularized testicular matrix (DTM) hydrogel and spermatogenesis...
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Xenograft and cell culture models of Sézary syndrome reveal cell of origin diversity and subclonal heterogeneity
Sézary Syndrome (SS) is a rare aggressive epidermotropic cutaneous T-cell lymphoma (CTCL) defined by erythroderma, pruritis, and a circulating...
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Three-dimensional culture models of human endometrium for studying trophoblast-endometrium interaction during implantation
During implantation, a symphony of interaction between the trophoblast originated from the trophectoderm of the implanting blastocyst and the...
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Phyto-factories of anti-cancer compounds: a tissue culture perspective
BackgroundCancer is one of the most critical but ubiquitous causes of death grappled from past decades. Widely used chemotherapy with cytotoxic...
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MRI-Based Cell Tracking of OATP-Expressing Cell Transplants by Pre-Labeling with Gd-EOB-DTPA
PurposeA critical step in cell-based therapies is determining the exact position of transplanted cells immediately post-transplant. Here, we devised...
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Contamination of CT scanner surfaces with SARS-CoV-2 and infective potential after examination of invasively ventilated, non-invasively ventilated and non-ventilated patients with positive throat swabs: prospective investigation using real-time reverse-transcription PCR and viral cell culture
BackgroundDuring the current severe acute respiratory syndrome coronavirus type 2 (SARS-CoV-2) pandemic, computed tomography (CT) has become widely...
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Diagnostic performance of metagenomic next-generation sequencing and conventional microbial culture for spinal infection: a retrospective comparative study
PurposeThe study evaluated the diagnostic performance of metagenomic next-generation sequencing (mNGS) as a diagnostic test for biopsy samples from...
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Knockdown of MRPL35 promotes cell apoptosis and inhibits cell proliferation in non-small-cell lung cancer
BackgroundNon-small cell lung cancer (NSCLC) is a major pathological type of lung cancer. However, its pathogenesis remains largely unclear. MRPL35...
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Prediction model for day 3 embryo implantation potential based on metabolites in spent embryo culture medium
BackgroundMetabolites in spent embryo culture medium correlate with the embryo’s viability. However, there is no widely accepted method using...
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Comparison of metagenomic next-generation sequencing and conventional culture for the diagnostic performance in febrile patients with suspected infections
BackgroundTimely and accurate identification of pathogens is crucial for appropriate treatment and prognosis of infectious diseases. As an...
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The effect of amorphous calcium carbonate as a culture media supplement on embryonic development of murine sibling embryos
PurposeThe aim of this study was to compare the addition in culture media of stabilized amorphous calcium carbonate (ACC) versus calcium chloride...
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Co-culture of human cryopreserved fragmented ovarian tissue with theca progenitor cells derived from theca stem cells
PurposeDespite the significant advances in the in vitro development of human primordial follicles, it is still a challenging approach with great...
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Development of in-vitro maturation protocol for rat oocytes; under simple culture vs co-culture with cumulus cell monolayer and its developmental potential via Parthenogenetic/artificial activation
BackgroundMurine is the most abundantly used as laboratory animal models. There has been a tremendous amount of research including; their evolution,...
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Genomic comparison between an in vitro three-dimensional culture model of melanoma and the original primary tumor
Three-dimensional (3D) melanoma culture is a personalized in vitro model that can be used for high-fidelity pre-clinical testing and validation of...
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Immunomodulatory effects of Diospyros peregrina fruit preparation (DFP) in non-small cell lung cancer (NSCLC) by utilizing dendritic cell-mediated antigen presentation and T helper (TH) cell differentiation
Diospyros peregrina is a dioecious plant which is native to India. It belongs to the family of Ebenaceae and is extensively used to treat various...
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Successful pregnancy with intracytoplasmic sperm injection after bacterial contamination of embryo culture in in vitro fertilization: a case report
BackgroundBacterial infection of embryo culture medium is rare but may be detrimental. The main source of embryo culture contamination is semen....
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ISSLS PRIZE in Basic Science 2024: superiority of nucleus pulposus cell- versus mesenchymal stromal cell-derived extracellular vesicles in attenuating disc degeneration and alleviating pain
PurposeTo investigate the therapeutic potential of extracellular vesicles (EVs) derived from human nucleus pulposus cells (NPCs), with a specific...
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Mini review: human clinical studies of stem cell therapy in keratoconus
Treatment of keratoconus is one of the most interesting research fields for researchers in the world. Regenerative medicine based on human stem cells...
