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SEM: Sample Preparation, Fixation, and Staining of Protists
The ultrastructure and morphology of the protist can be studied using high-resolution electron microscope, i.e., scanning electron microscope (SEM)....
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TEM: Sample Preparation, Fixation, and Staining of Protists
Transmission electron microscopy (TEM) is an ideal device to study the internal structure of cells and different types of biological materials, as...
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Conventional Sample Preparation
Collecting the samples for the preparation of histopathological slides is the great importance. In this step, care should be taken to avoid those...
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Sample Preparation for Electron Cryo-Microscopy of Macromolecular Machines
High-resolution structure determination by electron cryo-microscopy underwent a step change in recent years. This now allows study of challenging...
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Sample Preparation for Time-Resolved Serial Crystallography: Practical Considerations
Time-resolved serial crystallography is an emerging method to elucidate the structure-function relationship of biomolecular systems at up to atomic...
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Sample and Library Preparation for PacBio Long-Read Sequencing in Grapevine
PacBio long-read sequencing is a third-generation technology that generates long reads up to 20 kilobases (kb), unlike short-read sequencing...
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Electrospray-assisted cryo-EM sample preparation to mitigate interfacial effects
Addressing interfacial effects during specimen preparation in cryogenic electron microscopy remains challenging. Here we introduce ESI-cryoPrep, a...
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Sample Preparation for Fungal Community Analysis by High-Throughput Sequencing of Barcode Amplicons
Fungal species participate in vast numbers of processes in the landscape around us. However, their cryptic mycelial growth, inside various substrates...
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Selection of Suitable Methods for Sample Preparation and Desorbing Agents for Determination of Nutrient Contents in Scots Pine Roots
AbstractDetermining the nutrient composition of woody plant roots is an important tool for studying the physiological response of trees to various...
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High-throughput proteomic sample preparation using pressure cycling technology
High-throughput lysis and proteolytic digestion of biopsy-level tissue specimens is a major bottleneck for clinical proteomics. Here we describe a...
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Sample Preparation of Isolated Mitochondria for Cryoelectron Tomography and In Situ Studies of Translation
Cryoelectron tomography is a method to image biological samples three-dimensionally at molecular resolution. This modality provides insights into...
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Parasitic Helminth Sample Preparation for Taxonomic Study
Classical identificationClassical identification of parasitic helminthsParasitic helminths is traditionally based on differences of key morphological...
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Sample Preparation and Phosphopeptide Enrichment for Plant Phosphoproteomics via Label-Free Mass Spectrometry
Phosphopeptide enrichment is the main bottleneck of every phosphorylation study. Therefore, in this chapter, a general workflow tries to overbridge...
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Sample Preparation of Adherent Cell Lines for Flow Cytometry: Protocol Optimization—Our Experience with SW-480 colorectal cancer cell line
The most common method for detection of apoptosis is flow cytometry. In previously published studies there are some uncertainties and problems about...
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Multi-factorial examination of amplicon sequencing workflows from sample preparation to bioinformatic analysis
BackgroundThe development of sequencing technologies to evaluate bacterial microbiota composition has allowed new insights into the importance of...
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Sample Preparation for Mass Spectrometry-Based Absolute Quantification of Bacterial Proteins in Antibiotic Stress Research
Absolute protein quantificationAbsolute protein quantification is an essential tool for system biology approaches and elucidation of stoichiometry of...
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Combination of automated sample preparation and micro-flow LC–MS for high-throughput plasma proteomics
BackgroundNon-invasive detection of blood-based markers is a critical clinical need. Plasma has become the main sample type for clinical proteomics...
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Unconfined Compression Experimental Protocol for Cartilage Explants and Hydrogel Constructs: From Sample Preparation to Mechanical Characterization
Mechanical characterization of articular cartilage and cell-seeded hydrogel constructs is a challenging task due to the complex biphasic behavior of...
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Sample Preparation from Plant Tissue for Gas Chromatography–Mass Spectrometry (GC-MS)we
Metabolites are intermediate products formed during metabolism. Metabolites play different roles, including providing energy, supporting structure,...
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Measuring the Content of Endocannabinoid-Like Compounds in Biological Fluids: A Critical Overview of Sample Preparation Methodologies
Different mass spectrometric techniques have been used over the past decade to quantify endocannabinoids (eCBs) and related lipids. Even with the...