Conventional Sample Preparation

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Handbook of Techniques in Plant Histopathology

Abstract

Collecting the samples for the preparation of histopathological slides is the great importance. In this step, care should be taken to avoid those tissues with disease symptoms are altered by handling of the material (e.g. tweezers). Sample harveting for histopathological analysis is the most important stage. In this step, the researcher should be aware avoid those tissues with disease symptoms are altered by handling of the material (e.g. tweezers). In addition to diseased samples, always collect non-symptomatic samples: disease-free tissues to compare changes. It is considered an essential have samples closer to healthy tissue, without lesions or stress symptoms. The number of repetitions is essential. It is suggested that a minimum of three to four samples be collected from symptomatic and healthy tissues.

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Rodrigues Marques, J.P., Kasue Misaki Soares, M. (2022). Conventional Sample Preparation. In: Handbook of Techniques in Plant Histopathology. Springer, Cham. https://doi.org/10.1007/978-3-031-14659-6_2

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