Abstract
Collecting the samples for the preparation of histopathological slides is the great importance. In this step, care should be taken to avoid those tissues with disease symptoms are altered by handling of the material (e.g. tweezers). Sample harveting for histopathological analysis is the most important stage. In this step, the researcher should be aware avoid those tissues with disease symptoms are altered by handling of the material (e.g. tweezers). In addition to diseased samples, always collect non-symptomatic samples: disease-free tissues to compare changes. It is considered an essential have samples closer to healthy tissue, without lesions or stress symptoms. The number of repetitions is essential. It is suggested that a minimum of three to four samples be collected from symptomatic and healthy tissues.
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References
Berlyn GP, Miksche JP (1976) Botanical microtechnique and cytochemistry. Iowa State University, Ames, 326 p
De Micco V, Balzano A, Wheeler EA, Baas P (2016) Tyloses and gums: a review of structure, function and occurrence of vessel occlusions. IAWA J 37(2):186–205. https://doi.org/10.1163/22941932-20160130
Feder N, O’Brien TP (1968) Plant microtechnique: some principles and new methods. Am J Bot 55:123–142
Haupt AW (1930) A gelatin fixative for paraffin sections. Stain Technol 5:97–98
Huxley HE, Zubay GJ (1961) Preferential staining of nucleic acid-containing structures for electron microscopy. J Biophys Biochem Cytol 11:273–296. https://doi.org/10.1083/jcb.11.2.273
Johansen DA (1940) Plant microtechnique. McGraw-Hill Company Inc., New York, 523 p
Karnovsky MJ (1965) A formaldehyde-glutaraldehyde fixative of high osmolality for use in electron microscopy. J Cell Biol 27:137–138
Kraus JE, Arduin M (1997) Manual básico de métodos em morfologia vegetal. Editora Universidade Rural, Seropédica, Rio de Janeiro, 198 p
Marques JPR, Nuevo LG (2022) Double-staining method to detect pectin in plant-fungus interaction. J Vis Exp 180:e63432. https://doi.org/10.3791/63432
Marques JPR, Kitajima EW, Freitas-Astua J, Appezzato-da-Gloria B (2010) Comparative morpho-anatomical studies of the lesions caused by citrus leprosis virus on sweet orange. An Acad Bras Cienc 82:501–511. https://doi.org/10.1590/S0001-37652010000200025
Marques JPR, Hoy JW, Appezzato-da-Glória B, Gutierrez AF, Vieira MLC, Baisakh N (2018) Sugarcane cell wall-associated defense responses to infection by Sporisorium scitamineum. Front Plant Sci 9:698. https://doi.org/10.3389/fpls.2018.00698
Nakazono M, Qiu F, Borsuk LA, Schnable PS (2003) Laser-capture microdissection, a tool for the global analysis of gene expression in specific plant cell types: identification of genes expressed differentially in epidermal cells or vascular tissue of maize. Plant Cell 15:583–596. https://doi.org/10.1105/tpc.008102
Paiva EAS, Pinho SZ, Oliveira DMT (2011) Large plant samples: how to process for GMA embedding? In: Chiarini-Garcia H, Melo RCN (eds) Light microscopy: methods and protocols. Humana Press, Totowa, pp 37–49. https://doi.org/10.1007/978-1-60761-950-5_3
Sakai WS (1973) Simple method for differential staining of paraffin embedded plant material using toluidine blue O. Stain Technol 48:247–249. https://doi.org/10.3109/10520297309116632
Santos DC, Gregório EA, Machado SR (2007) Transmission electron microscopy applied to botany. Instituto de Biociências, UNESP, Botucatu, 36p
Sesso A (2011) Fixation of biological systems. In: Souza, W. Técnicas de microscopia eletronica aplicadas às ciências biológicas, 3ª edição. Rio de Janeiro: Sociedade Brasileira de Microscopia Eletrônica, 357p
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Rodrigues Marques, J.P., Kasue Misaki Soares, M. (2022). Conventional Sample Preparation. In: Handbook of Techniques in Plant Histopathology. Springer, Cham. https://doi.org/10.1007/978-3-031-14659-6_2
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DOI: https://doi.org/10.1007/978-3-031-14659-6_2
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