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Herbal species authentication by melting fingerprint coupled with high resolution melting analysis (MF-HRM)
BackgroundHerbal medicines have recently attracted increasing attention for use as food supplements with health benefits; however, species...
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High-Resolution Melting (HRM) analysis of DNA methylation using semiconductor chip-based digital PCR
BackgroundDigital PCR (dPCR) technology allows absolute quantification and detection of disease-associated rare variants, and thus the use of dPCR...
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Modified High-Resolution Melting (HRM) Marker Systems Increasing Discriminability Between Homozygous Alleles
Targeted single-nucleotide polymorphism (SNP) genoty**, especially for functional nucleotide polymorphism, is widely used for current breeding...
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Screening of Buffers and Additives for Protein Stabilization by Thermal Shift Assay: A Practical Approach
Thermal shift assay (TSA), also commonly designed by differential scanning fluorimetry (DSF) or ThermoFluor, is a technique relatively easy to...
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Tapioca: a platform for predicting de novo protein–protein interactions in dynamic contexts
Protein–protein interactions (PPIs) drive cellular processes and responses to environmental cues, reflecting the cellular state. Here we develop...
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Detection of SARS-CoV-2 spike protein D614G mutation using μTGGE
BackgroundThe accurate and expeditious detection of SARS-CoV-2 mutations is critical for monitoring viral evolution, assessing its impact on...
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In Vivo Optogenetic Phase Transition of an Intrinsically Disordered Protein
Proteins containing intrinsically disordered regions (IDRs) control a wide variety of cellular processes by assembly of membrane-less organelles via...
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Protein Interaction Analysis by Surface Plasmon Resonance
Surface plasmon resonance (SPR) is an optical technique that is utilized for detecting molecular interactions that occur in direct protein-protein...
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Protein Design Strategies for the Structural–Functional Studies of G Protein-Coupled Receptors
AbstractG protein-coupled receptors (GPCRs) are an important family of membrane proteins responsible for many physiological functions in human body....
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Aryliden-Imidazolones as Fluorogens of NanoLuc Protein
AbstractObjective: Fluorogens are synthetic molecules that become fluorescent in specific environments, i.e. in pockets of fluorogen-activating...
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Applications of Circular Dichroism Spectroscopy in Studying Protein Folding, Stability, and Interaction
Circular dichroism (CD) spectroscopy has been extensively used to determine the structure and folding of proteins. It provides valuable information...
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Early intermediates in bacterial RNA polymerase promoter melting visualized by time-resolved cryo-electron microscopy
During formation of the transcription-competent open complex (RPo) by bacterial RNA polymerases (RNAPs), transient intermediates pile up before...
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Using Intrinsic Fluorescence to Measure Protein Stability Upon Thermal and Chemical Denaturation
Understanding how point mutations affect the performance of protein stability has been the focus of several studies all over the years. Intrinsic...
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Physical Basis of Functioning of Antifreeze Protein
AbstractAntifreeze proteins, expressed in cold-blooded organisms, prevent ice formation in their bodies, and thus help them to survive in extremely...
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Rewiring of the protein–protein–metabolite interactome during the diauxic shift in yeast
In budding yeast Saccharomyces cerevisiae , the switch from aerobic fermentation to respiratory growth is separated by a period of growth arrest,...
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Directed-evolution mutations enhance DNA-binding affinity and protein stability of the adenine base editor ABE8e
Adenine base editors (ABEs), consisting of CRISPR Cas nickase and deaminase, can chemically convert the A:T base pair to G:C. ABE8e, an evolved...
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Design of complicated all-α protein structures
A wide range of de novo protein structure designs have been achieved, but the complexity of naturally occurring protein structures is still far...
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Protein Biosynthesis and Maturation in the ER
The endoplasmic reticulum takes care of the folding, assembly, and quality control of thousands of proteins destined to the different compartments of...
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Biophysical Approaches for the Characterization of Protein-Metabolite Interactions
With an estimate of hundred thousands of protein molecules per cell and the number of metabolites several orders of magnitude higher,...
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Principles, Methods, and Applications of Protein Folding Inside Cells
The complex mechanism of protein folding in cells has intrigued the scientific community for decades. The physical and chemical forces that drive...