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Computational Evaluation of Peroxisomal Targeting Signals in Metazoa
Most soluble proteins enclosed in peroxisomes encode either type-1 or type-2 peroxisomal targeting signals (PTS1 or PTS2), which act as postal codes...
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A Cell-Free In Vitro Import System for Peroxisomal Proteins Containing a Type 2 Targeting Signal (PTS2)
Cell-free in vitro systems are invaluable tools to study the molecular mechanisms of protein translocation across biological membranes. We have been...
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Chaperone proteins and peroxisomal protein import
Peroxisomes are ubiquitous organelles present in most eukaryotic cells. Their role in cellular metabolism is diverse among species. An array of genes...
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Assay of Reactive Oxygen/Nitrogen Species (ROS/RNS) in Arabidopsis Peroxisomes Through Fluorescent Protein Containing a Type 1 Peroxisomal Targeting Signal (PTS1)
Plant peroxisomes have an active nitro-oxidative metabolism. However, the assay of reactive oxygen and nitrogen species (ROS/RNS) could be a...
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Computational Approaches for Peroxisomal Protein Localization
Computational approaches are practical when investigating putative peroxisomal proteins and for sub-peroxisomal protein localization in unknown...
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Fusarium verticillioides Pex7/20 mediates peroxisomal PTS2 pathway import, pathogenicity, and fumonisin B1 biosynthesis
AbstractFusarium verticillioides , a well-known fungal pathogen that causes severe disease in maize and contaminates the grains with fumonisin B1...
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Identification of Auxiliary Organellar Targeting Signals for Plant Peroxisomes Using Bioinformatic Analysis of Large Protein Sequence Datasets Followed by Experimental Validation
Eukaryotic cells are compartmentalized by membrane-bounded organelles to ensure that specific biochemical reactions and cellular functions occur in a...
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Using the Superfolder GFP (sfGFP) System to Study Plant Peroxisomal Protein Import
The fusing of a protein of interest to a fluorescent protein followed by fluorescence microscopy is a very common method of determining protein...
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Mouse Models to Study Peroxisomal Functions and Disorders: Overview, Caveats, and Recommendations
During the last three decades many mouse lines were created or identified that are deficient in one or more peroxisomal functions. Different...
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Functional insights of three RING-finger peroxins in the life cycle of the insect pathogenic fungus Beauveria bassiana
Peroxisomes play important roles in fungal physiological processes. The RING-finger complex consists of peroxins Pex2, Pex10, and Pex12 and is...
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Analysis of Yeast Peroxisomes via Spatial Proteomics
Peroxisomes are ubiquitous organelles with essential functions in numerous cellular processes such as lipid metabolism, detoxification of reactive...
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Estimating the Interaction Strength Between PTS1-Peptides and Their Receptor PEX5 in Living Cells Using Flow-Cytometer-Based FRET (flowFRET) Measurements
The import of many peroxisomal matrix proteins is initiated by the interaction of type-1 peroxisomal targeting signals (PTS1) residing at the extreme...
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Aspergillus niger uses the peroxisomal CoA-dependent β-oxidative genes to degrade the hydroxycinnamic acids caffeic acid, ferulic acid, and p-coumaric acid
AbstractAromatic compounds are important molecules which are widely applied in many industries and are mainly produced from nonrenewable sources....
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Anaerobic derivates of mitochondria and peroxisomes in the free-living amoeba Pelomyxa schiedti revealed by single-cell genomics
BackgroundMitochondria and peroxisomes are the two organelles that are most affected during adaptation to microoxic or anoxic environments....
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Manipulation and Visualization of Peroxisomes in Drosophila
Drosophila melanogaster is a proven metazoan model to investigate the fundamentals of human genetic diseases including peroxisome-related disorders....
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Quantitative subcellular reconstruction reveals a lipid mediated inter-organelle biogenesis network
The structures and functions of organelles in cells depend on each other but have not been systematically explored. We established stable knockout...
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mRNA Targeting, Transport and Local Translation in Eukaryotic Cells: From the Classical View to a Diversity of New Concepts
AbstractSpatial organization of protein biosynthesis in the eukaryotic cell has been studied for more than fifty years, thus many facts have already...
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Peroxisome biogenesis and inter-organelle communication: an indispensable role for Pex11 and Pex30 family proteins in yeast
Peroxisomes are highly dynamic organelles present in most eukaryotic cells. They also play an important role in human health and the optimum...
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Biosynthese, Prozessierung und Abbau von Proteinen in Pflanzen
Während der Protein-Biosynthese wird die Nukleotidsequenz der mRNA in eine Aminosäuresequenz übersetzt (translatiert). Als Übersetzer wirken dabei...
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MrPEX33 is involved in infection-related morphogenesis and pathogenicity of Metarhizium robertsii
AbstractPeroxisomes, being indispensable organelles, play an important role in different biological processes in eukaryotes. PEX33, a filamentous...
