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Comparative assessment between liquid culture in static and temporary immersion systems on multiplication, morpho-physiological and biochemical characteristics of Bambusa vulgaris Schrad. ex Wendl. shoots
Key messageShoots of B. vulgaris cultured in TIS showed better morpho-physiological and biochemical characteristics, without hyperhydricity during...
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Static 3D Osteoblast Cell Culture on 3D Printed Titanium Scaffolds
In vitro cell cultures are a very useful tool for the validation of biomaterial cytocompatibility, especially for bone tissue engineering scaffolds...
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Antibiofilm activity from endophyte bacteria, Vibrio cholerae strains, and actinomycetes isolates in liquid and solid culture
BackgroundBiofilm-associated infections are a global threat to our economy and human health; as such, development of antibiofilm compounds is an...
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Establishment of Physalis alkekengi cell suspension culture: time-dependent behavior of genes related to the steroidal compounds, key enzymes, and physalins under static magnetic field
Cell suspension culture has the potential to be a valuable source for the bioactive compound productions. In this study, an optimized procedure was...
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Anatomical and biochemical changes of shoots of Bambusa vulgaris Schrad. ex Wendl under different in vitro shoot culture systems
In vitro propagation by organogenesis of Bambusa vulgaris Schrad. ex Wendl ( B. vulgaris ) is hampered by the low survival rate of the plants under ex...
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Three-Dimensional Rotary Culture to Model Mycobacterial Biofilms in Low-Shear Detergent-Free Liquid Suspension
In vitro biofilm models have allowed researchers to investigate the role biofilms play in the pathogenesis, virulence, and antimicrobial drug...
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Assaying Proliferation Characteristics of Cells Cultured Under Static Versus Periodic Conditions
Two-dimensional in vitro culture models are widely being employed for assessing a vast variety of biological questions in different scientific...
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Co-culture engineering: a promising strategy for production of engineered extracellular vesicle for osteoarthritis treatment
The therapeutic effects of extracellular vesicles (EVs) have been identified as a significant factor in intercellular communication in different...
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Enhancement of cordycepin production from Cordyceps militaris culture by epigenetic modification
Cordycepin (3′-deoxyadenosine) is a nucleoside analogue and biosynthesised by Cordyceps militaris , an entomopathogenic fungus. In this study, an...
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Cell Culture Laboratory
The first objective of cell culture laboratory is to maintain hygiene and keep it contamination-free. The design of a cell culture laboratory majorly...
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Adipose-derived stem cells (ASCs) culture in spinner flask: improving the parameters of culture in a microcarrier-based system
Prior to clinical use, extensive in vitro proliferation of human adipose-derived stem cells (ASCs) is required. Among the current options,...
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Co-culture Techniques
Co-culturing cells in in vitro tissue models is a common technique for investigating how they interact. These models have been used to simulate a...
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Bacterial cellulose production by Komagataeibacter hansenii can be improved by successive batch culture
Bacterial cellulose (BC) is a biopolymer principally synthetized by strains of the genus Komagataeibacter . However, high costs and low production...
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A forced aeration system for microbial culture of multiple shaken vessels suppresses volatilization
Shake-flask culture, an aerobic submerged culture, has been used in various applications involving cell cultivation. However, it is not designed for...
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3D Cell Culture: Techniques For and Beyond Organoid Applications
In the rapidly evolving landscape of cell biology and biomedical research, three-dimensional (3D) cell culture has contributed not only to the...
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Effect of Cell-Free Culture Fluid of Staphylococcus aureus on the Structure and Biochemical Composition of Klebsiella pneumoniae and Pseudomonas aeruginosa Biofilms
AbstractRecently, there has been increasing evidence that many infections are associated with the formation of multispecies biofilms, in which there...
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Direct Shoot From Root and True-to-Type Micropropagation of Limonium “Misty Blue” in Partially Immersed Culture on an Aluminum Mesh Raft
Commercial plant tissue culture now primarily serves the ornamental horticulture industry. The main pillars of the commercial tissue culture business...
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Detection of “Non-culturable” Mycobacterium tuberculosis Cells by Culture Methods
AbstractThe specific features of the reactivation of “non-culturable” (NC) Mycobacterium tuberculosis (MTB) cells for the following multiplication...
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Tissue Culture and Somatic Fusion in Plants
Molecular biology is a great avenue to combat climatic risks and challenges in agriculture. Interventions have improved the seasonal fluctuation in...
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Bioreactor Culture to Create Adipose Tissue from Human Mesenchymal Stromal Cells
Adipose tissue is a complex and multifaceted endocrine organ located throughout the body. The dysfunction of adipose tissue is known to induce a wide...