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Western Blotting
Western blot (also known as protein immunoblot) is an important molecular biology technique routinely used in most research laboratories for the...
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Western Blotting
Western blotting is used to transfer proteins from polyacrylamide gels (PAGs) to the membranes, making its identification and determination more...
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Southern and Northern Blotting
Blotting techniques are among the most common molecular biology approaches to immobilize proteins or nucleic acid (DNA/RNA) separation using a...
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Validating Antibody Specificities for Immunohistochemistry by Protein Blotting
Optimized antibody reagents are important in research, and erratic antibody performance leads to variability in immunoassays. Specificity of...
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Cytotoxicity, apoptosis inducing activity and Western blot analysis of tanshinone derivatives from Stachys parviflora on prostate and breast cancer cells
Cytotoxic activities of methanolic crude extract of Stachys parviflora (Lamiaceae family) and its sub-fractions were primarily evaluated against...
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ZmAGO18b negatively regulates maize resistance against southern leaf blight
Key messageHere, we report that ZmAGO18b encoding an argonaute protein is a negative regulator of maize resistance against southern leaf blight.
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Preclinical Western Blot in the Era of Digital Transformation and Reproducible Research, an Eastern Perspective
AbstractThe current research is an interdisciplinary endeavor to develop a necessary tool in preclinical protein studies of diseases or disorders...
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Engineering of ketocarotenoid biosynthetic pathway in Chlamydomonas reinhardtii through exogenous gene expression
Microalgal biorefineries have emerged as significant reservoirs of therapeutic compounds, including pigments and proteins. Facilitating a robust...
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Simple Western: Bringing the Western Blot into the Twenty-First Century
The Western blot is widely used in the study of protein biochemistry, but it is notoriously labor-intensive, and it is limited in its reproducibility...
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CircHAT1 regulates the proliferation and phenotype switch of vascular smooth muscle cells in lower extremity arteriosclerosis obliterans through targeting SFRS1
This study aimed to decipher the mechanism of circular ribonucleic acids (circRNAs) in lower extremity arteriosclerosis obliterans (LEASO). First,...
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Exosomal PGAM1 promotes prostate cancer angiogenesis and metastasis by interacting with ACTG1
Tumor-derived exosomes and their contents promote cancer metastasis. Phosphoglycerate mutase 1 (PGAM1) is involved in various cancer-related...
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A murine monoclonal antibody against H5N1 avian influenza virus cross-reacts with human kidney cortex cells
To investigate the biological characteristics of monoclonal antibodies (mAbs) against avian influenza virus (AIV) and the possible mechanism of...
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In vitro propagation via indirect shoot organogenesis, assessment of genetic stability using SCoT markers and antioxidant analysis in Barleria prattensis Sant., an endemic medicinal plant to the Western Ghats
An efficient shoot regeneration system has been developed via indirect shoot organogenesis from leaf petiole-derived callus in Barleria prattensis ...
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Long Non-Coding RNA PCAT19 Suppresses Cell Proliferation and Angiogenesis in Coronary Artery Disease through Interaction with GCNT2
LncRNAs involvement in heart disease, however, the effect of lncRNA prostate cancer‐associated transcript 19 (PCAT19) in coronary artery disease...
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Improving the accumulation of recombinant human serum albumin (HSA) in transgenic tobacco plants by fusion with the N-terminal proline-rich domain of γ-zein (Zera)
Plants have long played a major role in human health as a source of herbal remedies. Recently, transgenic plants have become convenient bioreactors...
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Seed-specific expression of porcine verotoxigenic Escherichia coli antigens in tobacco plants as a potential model of edible vaccines
Vaccines can reduce the use of antibiotics by preventing specific infective diseases in pigs. Plant-based edible vaccines are particularly attractive...
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Coat protein–mediated resistance to cucumber mosaic virus subgroup IB in black pepper (Piper nigrum L.)
Cucumber mosaic virus (CMV) infects a large number of plant species including Piper nigrum L. and related species. As natural resistance to CMV is...
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ERS Mediated by GRP-78/PERK/CHOP Signaling Is Involved in Fluoride-Induced Ameloblast Apoptosis
Fluoride can be widely ingested from the environment, and its excessive intake could result in adverse effects. Dental fluorosis is an early sign of...
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Knockdown of trem2 promotes proinflammatory microglia and inhibits glioma progression via the JAK2/STAT3 and NF-κB pathways
BackgroundIn the tumor immune microenvironment (TIME), triggering receptor expressed on myeloid cells 2 (trem2) is widely considered to be a crucial...
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Research on the Effect and Mechanism of Selenium on Colorectal Cancer Through TRIM32
The intake of selenium (Se) in the human body is negatively correlated with the risk of colorectal cancer (CRC), but its mechanism in the occurrence...
