Abstract
Blotting techniques are among the most common molecular biology approaches to immobilize proteins or nucleic acid (DNA/RNA) separation using a membrane. Samples can be detected by specific ligands, antibodies, or nucleic acid probes, which bind with individual nucleic acid sequences or proteins. These techniques depend upon the resolving ability of the individual macromolecules with respect to their size. The most well-recognized application of blotting is the Southern blot and Western blot, in which nucleic acid/proteins are immobilized on PVDF or nitrocellulose membranes. Blotting technology has been utilized over the last 30 years to elucidate many fundamental biological processes and promises more excellent discoveries in molecular biology. This chapter discussed the principle and methodology of the different types of blotting techniques.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Botchan, M., Topp, W., & Sambrook, J. (1976). The arrangement of simian virus 40 sequences in the DNA of transformed cells. Cell, 9, 269–287.
Botstein, D., White, R. L., Skolnick, M., & Davis, R. W. (1980). Construction of a genetic linkage map in man using restriction fragment length polymorphisms. American Journal of Human Genetics, 32, 314–331.
Brewer, B. J., & Fangman, W. L. (1987). The localization of replication origins on ARS plasmids in S. cerevisiae. Cell, 51, 463–471.
Denhardt, D. T. (1966). A membrane-filter technique for the detection of complementary DNA. Biochemical and Biophysical Research Communications, 23, 641–646.
Jeffreys, A. J., & Flavell, R. A. (1977). The rabbit β-globin gene contains a large large insert in the coding sequence. Cell, 12, 1097–1108.
Jeffreys, A. J., Wilson, V., & Thein, S. L. (1985). Individual-specific ‘fingerprints’ of human DNA. Nature, 316, 76–79.
Kan, Y. W., & Dozy, A. M. (1978). Polymorphism of DNA sequence adjacent to human beta-globin structural gene: Relationship to sickle mutation. Proceedings of the National Academy of Sciences of the USA, 75, 5631–5635.
Sambrook, J., Fritsch, E. F., & Maniatis, T. (1989). Cold Spring Harbor. Cold Spring Harbor Laboratory Press.
Southern, E. (2006). Southern blotting. Nature Protocols, 1(2), 518–525.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
Copyright information
© 2022 The Author(s), under exclusive license to Springer Nature Switzerland AG
About this chapter
Cite this chapter
Gautam, A. (2022). Southern and Northern Blotting. In: DNA and RNA Isolation Techniques for Non-Experts. Techniques in Life Science and Biomedicine for the Non-Expert. Springer, Cham. https://doi.org/10.1007/978-3-030-94230-4_21
Download citation
DOI: https://doi.org/10.1007/978-3-030-94230-4_21
Published:
Publisher Name: Springer, Cham
Print ISBN: 978-3-030-94229-8
Online ISBN: 978-3-030-94230-4
eBook Packages: Biomedical and Life SciencesBiomedical and Life Sciences (R0)