Search
Search Results
-
The critical role of a conserved lysine residue in periplasmic nitrate reductase catalyzed reactions
Periplasmic nitrate reductase NapA from Campylobacter jejuni ( C. jejuni ) contains a molybdenum cofactor (Moco) and a 4Fe–4S cluster and catalyzes the...
-
Dynamic interplay between the periplasmic chaperone SurA and the BAM complex in outer membrane protein folding
Correct folding of outer membrane proteins (OMPs) into the outer membrane of Gram-negative bacteria depends on delivery of unfolded OMPs to the...
-
The periplasmic chaperone protein Psg_2795 contributes to the virulence of Pseudomonas savastanoi pv. glycinea: the causal agent of bacterial blight of soybean
Pseudomonas savastanoi pv. glycinea (Psg also named P. syringae pv. glycinea and P. amygdali pv. glycinea) is the causative agent of bacterial blight...
-
In silico and in vitro analysis of a rational mutation in gIII signal peptide and its effects on periplasmic expression of rhGH in E. coli
The secretion efficiency of a heterologous protein in E. coli is mainly dictated by the N-terminal signal peptide fused to the desired protein. In...
-
Expression of a periplasmic β-carbonic anhydrase (CA) gene is positively correlated with HCO3- utilization by the gametophytes of Saccharina japonica (Phaeophyceae, Ochrophyta)
As an ecologically and economically important seaweed, Saccharina japonica has developed strategies to utilize HCO 3 - . In general, seaweeds have at...
-
Identification of enzymatic functions of osmo-regulated periplasmic glucan biosynthesis proteins from Escherichia coli reveals a novel glycoside hydrolase family
Most Gram-negative bacteria synthesize osmo-regulated periplasmic glucans (OPG) in the periplasm or extracellular space. Pathogenicity of many...
-
Defining Membrane Protein Topology Using pho-lac Reporter Fusions
Experimental determination of membrane protein topology can be achieved using various techniques. Here, we present the pho-lac dual reporter system,...
-
Structural insights into the mechanism of protein transport by the Type 9 Secretion System translocon
Secretion systems are protein export machines that enable bacteria to exploit their environment through the release of protein effectors. The Type 9...
-
Directed evolution of tripartite ATP-independent periplasmic transporter for 3-Hydroxypropionate biosynthesis
AbstractOur previous study’s introduction of the malonic acid assimilation pathway into Escherichia coli enabled biosynthesis of 3-Hydroxypropionate...
-
Crystal structure and metal binding properties of the periplasmic iron component EfeM from Pseudomonas syringae EfeUOB/M iron-transport system
EfeUOB/M has been characterised in Pseudomonas syringae pathovar. syringae as a novel type of ferrous-iron transporter, consisting of an...
-
Protein–Protein Interactions: Oxidative Bacterial Two Hybrid
Protein–protein interaction studies are essential to understand how proteins organize themselves into interaction networks and thus influence...
-
De Novo Designing a Novel Signal Peptide for Secretion of Neurturin to the Periplasmic Space of Escherichia coli
AbstractNeurotrophic factors, including neurturin, increase the life span of the neurons and prevent neuronal destruction. As recombinant expression...
-
Protein-Protein Binding Kinetics by Biolayer Interferometry
The specific kinetics and thermodynamics of protein-protein interactions underlie the molecular mechanisms of cellular functions; hence the...
-
When less is more: shortening the Lpp protein leads to increased vancomycin resistance in Escherichia coli
Vancomycin is a naturally occurring cell-wall-targeting glycopeptide antibiotic. Due to the low potency of this antibiotic against Gram-negative...
-
Periplasmic synthesis and purification of the human prolactin antagonist Δ1-11-G129R-hPRL
The human prolactin antagonist Δ 1-11 -G129R-hPRL is a 21.9 kDa recombinant protein with 188 amino acids that downregulates the proliferation of a...
-
Relocation of dehydroquinate dehydratase to the periplasmic space improves dehydroshikimate production with Gluconobacter oxydans strain NBRC3244
Abstract3-Dehydroshikimate (3-DHS) is a key intermediate for the synthesis of various compounds, including the antiviral drug oseltamivir. The Glucono...
-
Identification of Protein Partners by APEX2 Proximity Labeling
Proximity labeling methods enable the identification of proteins in the vicinity of a protein of interest in living cells. Among them, APEX2...
-
Protein Sorting Prediction
Many computational methods are available for predicting protein sorting in bacteria. When comparing them, it is important to know that they can be...
-
Protein Secretion
Bacterial cells (gram positive and negative) utilize generalized secretory pathways such as Sec and Tat pathways that are ubiquitous to all domains...
-
Compartment-related aspects of XoxF protein functionality in Methylorubrum extorquens DM4 analysed using its cytoplasmic targeting
The impact of periplasmic localisation on the functioning of the XoxF protein was evaluated in the well-studied dichloromethane-utilising...
