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1. Rapid Ribonuclease P Kinetics Measured by Stopped-Flow Fluorescence and Fluorescence Anisotropy

   Stopped-flow fluorescence spectroscopy is a highly sensitive method for measuring rapid enzyme kinetics. A wide range of fluorophores can be...

   Alexandra R. Chamberlain, Michael E. Harris in RNA Amplification and Analysis

   Protocol 2024
   

2. Enzyme Kinetics

   In this chapter, you will learn the fundamental concepts of chemical and enzyme catalysis as well as kinetics such as the lock and key and the...

   Ana Malvis Romero, Lorenzo Pesci, ... Andreas Liese in Introduction to Enzyme Technology

   Chapter 2024
   

3. Phosphorus absorption kinetics and exudation strategies of roots developed by three lupin species to tackle P deficiency

   Main conclusion

   Different lupin species exhibited varied biomass, P allocation, and physiological responses to P-deprivation. White and yellow lupins...

   Ruixin Wang, Sachiko Funayama-Noguchi, ... Jun Wasaki in Planta

   Article 22 December 2023
   

4. Protein-Protein Binding Kinetics by Biolayer Interferometry

   The specific kinetics and thermodynamics of protein-protein interactions underlie the molecular mechanisms of cellular functions; hence the...

   Jorge Santos-López, Sara Gómez, ... M. Cristina Vega in Advanced Technologies for Protein Complex Production and Characterization

   Chapter 2024
   

5. Microbial Kinetics

   It’s all about fluxes and rates! Environmental resources are available to microbes in form of fluxes of energy and matter. Some important intrinsic...

   Alfred M. Spormann in Principles of Microbial Metabolism and Metabolic Ecology

   Chapter 2023
   

6. Enzyme Kinetics: A Plethora of Information

   The study of enzyme kinetics has been the fundamental basis of science to understand the reaction mechanism. The most commonly studied model is the...

   Komal Agrawal, Pradeep Verma in Industrial Microbiology and Biotechnology

   Chapter 2022
   

7. Leukemia circulation kinetics revealed through blood exchange method

   Leukemias and their bone marrow microenvironments undergo dynamic changes over the course of disease. However, little is known about the circulation...

   Alex B. Miller, Felicia H. Rodriguez, ... Scott R. Manalis in Communications Biology

   Article Open access 20 April 2024
   

8. Long-term memory in Staphylococcus aureus α-hemolysin ion channel kinetics

   The kinetics of an ion channel are classically understood as a random process. However, studies have shown that in complex ion channels, formed by...

   M. P. Silva, C. G. Rodrigues, ... R. A. Nogueira in European Biophysics Journal

   Article 05 August 2023
   

9. Quantifying the Kinetics of Pilus-Specific Sortase-Catalyzed Crosslinking Using High-Performance Liquid Chromatography

   Gram-positive bacteria display pili whose protein components (pilins) are covalently crosslinked by pilus-specific sortase enzymes. These cysteine...

   Nicole A. Cheung, Mabel Song, ... Robert T. Clubb in The Bacterial Cell Wall

   Protocol 2024
   

10. Flash-kinetics as a complementary analytical tool in PAM fluorimetry

    A new measuring system based on the already existing Multi-Color-PAM Fluorimeter (Schreiber et al. in Photosynth Res 113:127–144, 2012) was developed...

    Christof Klughammer, Friedemann Schlosser, Ulrich Schreiber in Photosynthesis Research

    Article Open access 22 May 2024
    

11. Glutamate indicators with improved activation kinetics and localization for imaging synaptic transmission

    The fluorescent glutamate indicator iGluSnFR enables imaging of neurotransmission with genetic and molecular specificity. However, existing iGluSnFR...

    Abhi Aggarwal, Rui Liu, ... Kaspar Podgorski in Nature Methods

    Article Open access 04 May 2023
    

12. The Mechanism of Inhibition of Mycobacterial (p)ppGpp Synthetases by a Synthetic Analog of Erogorgiaene

    Abstract

    The synthesis of (p)ppGpp alarmones plays a vital role in the regulation of metabolism suppression, growth rate control, virulence, bacterial...

    Roman Y. Sidorov, Alexander G. Tkachenko in Biochemistry (Moscow)

    Article 01 March 2024
    

13. Fertilisation kinetics among common Indo-Pacific broadcast spawning corals with distinct and shared functional traits

    Indo-Pacific corals predominantly reproduce using synchronous mass spawning events to maximise fertilisation. However, as disturbances continue to...

    Elizabeth Buccheri, Gerard F. Ricardo, ... Christopher Doropoulos in Coral Reefs

    Article Open access 28 October 2023
    

14. SBMLKinetics: a tool for annotation-independent classification of reaction kinetics for SBML models

    Background

    Reaction networks are widely used as mechanistic models in systems biology to reveal principles of biological systems. Reactions are...

    ** Xu in BMC Bioinformatics

    Article Open access 13 June 2023
    

15. Mesoscopic kinetics and its applications in protein synthesis

    Molecular biology emerged through unification of genetics and nucleic acid chemistry that took place with the discovery of the double helix (Watson...

    Johan Elf, Johan Paulsson, ... Måns Ehrenberg in Systems Biology

    Chapter
    

16. Temporal microbial succession drives phase-dependent kinetics of di(2-ethylhexyl) phthalate biodegradation in soil

    Di(2-ethylhexyl) phthalate (DEHP), one of the most important plasticizers, is considered a typical endocrine disruptor. Soil contains high levels of...

    Ruiwen Hu, Songfeng Liu, ... Cheng Wang in Biology and Fertility of Soils

    Article 09 May 2023
    

17. The Effect of Chemical Chaperones on Proteins with Different Aggregation Kinetics

    Abstract

    Formation and accumulation of protein aggregates adversely affect intracellular processes in living cells and are negative factors in the...

    Valeriya V. Mikhaylova, Tatiana B. Eronina, ... Boris I. Kurganov in Biochemistry (Moscow)

    Article 01 January 2023
    

18. Dissecting key regulators of transcriptome kinetics through scalable single-cell RNA profiling of pooled CRISPR screens

    We present a combinatorial indexing method, PerturbSci-Kinetics , for capturing whole transcriptomes, nascent transcriptomes and single guide RNA...

    Zihan Xu, Andras Sziraki, ... Junyue Cao in Nature Biotechnology

    Article Open access 25 September 2023
    

19. Kinetics of dengue viremia and its association with disease severity: an ambispective study

    Background

    Dengue virus (DENV) infection is an important public health problem and causes significant morbidity and mortality. DENV typically causes a...

    Puneet Bhatt, Anup Jayaram, ... Chiranjay Mukhopadhyay in VirusDisease

    Article Open access 29 June 2024
    

20. Modelling Release Kinetics and Path Analysis of Phosphorus in Acid Soil as Influenced by Phosphorus Solubilizers

    Application of citric acid (CA), Bacillus polymyxa (PB), and lime (CaCO ₃ , LI) may enhance P availability to plants by reducing the fixation of...

    Dibyendu Chatterjee, Konsam Vikramjeet, ... Saikat Ranjan Das in Journal of Soil Science and Plant Nutrition

    Article 01 September 2022