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Engineered domain-inlaid Nme2Cas9 adenine base editors with increased on-target DNA editing and targeting scope
BackgroundNme2ABE8e has been constructed and characterized as a compact, accurate adenine base editor with a less restrictive dinucleotide...
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High-throughput continuous evolution of compact Cas9 variants targeting single-nucleotide-pyrimidine PAMs
Despite the availability of Cas9 variants with varied protospacer-adjacent motif (PAM) compatibilities, some genomic loci—especially those with...
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Versatile and efficient genome editing with Neisseria cinerea Cas9
The CRISPR/Cas9 system is a versatile genome editing platform in biotechnology and therapeutics. However, the requirement of protospacer adjacent...
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Evolutionary mining and functional characterization of TnpB nucleases identify efficient miniature genome editors
As the evolutionary ancestor of Cas12 nuclease, the transposon (IS200/IS605)-encoded TnpB proteins act as compact RNA-guided DNA endonucleases. To...
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Approaches to Therapeutic Gene Editing in Alpha-1 Antitrypsin Deficiency
Five distinct gene therapy approaches have been developed for treating AATD. These approaches include knockout of the mutant (PiZ) allele by...
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Introduction and Perspectives of DNA Base Editors
DNA base editors, one of the CRISPR-based genome editing tools, can induce targeted point mutations at desired sites. Their superiority is based on...
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A multifunctional Wnt regulator underlies the evolution of rodent stripe patterns
Animal pigment patterns are excellent models to elucidate mechanisms of biological organization. Although theoretical simulations, such as Turing...
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Efficient and high-fidelity base editor with expanded PAM compatibility for cytidine dinucleotide
Cytidine base editor (CBE), which is composed of a cytidine deaminase fused to Cas9 nickase, has been widely used to induce C-to-T conversions in a...
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Anti-CRISPR proteins: a weapon of phage-bacterial arm race for genome editing
The ongoing evolutionary competition between bacteria and their phage parasites has led to the development of intricate systems of attacks, defenses,...
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Evolution of an adenine base editor into a small, efficient cytosine base editor with low off-target activity
Cytosine base editors (CBEs) are larger and can suffer from higher off-target activity or lower on-target editing efficiency than current adenine...
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Anti-CRISPR protein applications: natural brakes for CRISPR-Cas technologies
Clustered, regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated (Cas) genes, a diverse family of prokaryotic adaptive...
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Base Editors-Mediated Gene Therapy in Hematopoietic Stem Cells for Hematologic Diseases
Base editors, developed from the CRISPR/Cas system, consist of components such as deaminase and Cas variants. Since their emergence in 2016, the...
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Wide Horizons of CRISPR-Cas-Derived Technologies for Basic Biology, Agriculture, and Medicine
The discovery and subsequent repurposing of the CRISPR (clustered regularly interspaced short palindromic repeats) and Cas proteins...
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The applications of CRISPR/Cas-mediated genome editing in genetic hearing loss
Hearing loss (HL) can be caused by a number of different genetic factors. Non-syndromic HL refers that HL occurs as an isolated symptom in an...
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Precise base editing with CC context-specificity using engineered human APOBEC3G-nCas9 fusions
BackgroundCytidine base editors (CBEs), composed of a cytidine deaminase fused to Cas9 nickase (nCas9), enable efficient C-to-T conversion in various...
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Efficient base editing with high precision in rabbits using YFE-BE4max
Cytidine base editors, composed of a cytidine deaminase fused to Cas9 nickase, enable efficient C-to-T conversion in various organisms. However,...
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Recent developments and applications of genetic transformation and genome editing technologies in wheat
Wheat ( Triticum aestivum ) is a staple crop across the world and plays a remarkable role in food supplying security. Over the past few decades, basic...
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Broad-spectrum anti-CRISPR proteins facilitate horizontal gene transfer
CRISPR–Cas adaptive immune systems protect bacteria and archaea against their invading genetic parasites, including bacteriophages/viruses and...
