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Kluyveromyces lactis zymocin and other plasmid-encoded yeast killer toxins
Supported by an endogenous killer plasmid DNA system that codes for an anti-proliferative zymocin toxin, the dairy yeast Kluyveromyces lactis has...
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Molecular Genetic and Functional Analysis of the Rap-Phr Signal System of the Plasmid pBS72 of Bacillus subtilis Isolates
Abstract —The functional characteristics of the Rap‒Phr quorum sensing signal system of the plasmid pBS72 were investigated. Phylogenetic relationship...
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The Effect of the flhB Plasmid Gene of the Flagellar Export Component on Flagellation and Motility in Azospirillum Bacteria
The effect was analyzed of the flhB2 gene, which is located on the AZOBR_p4 (AZOBR_p410073 gene) plasmid in Azospirillum baldaniorum Sp245 and on the...
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Engineering plasmid copy number heterogeneity for dynamic microbial adaptation
Natural microbial populations exploit phenotypic heterogeneity for survival and adaptation. However, in engineering biology, limiting the sources of...
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Conjugative type IV secretion systems enable bacterial antagonism that operates independently of plasmid transfer
Bacterial cooperation and antagonism mediated by secretion systems are among the ways in which bacteria interact with one another. Here we report the...
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Elimination of editing plasmid mediated by theophylline riboswitch in Zymomonas mobilis
AbstractZymomonas mobilis is regarded as a potential chassis for the production of platform chemicals. Genome editing using the CRISPR-Cas system...
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Evaporation-induced hydrodynamics control plasmid transfer during surface-associated microbial growth
Droplet evaporation is a general process in unsaturated environments that results in micro-scale hydrodynamic flows which in turn determine the...
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Digital PCR: a tool in clostridial mutant selection and detection
AbstractThe ClosTron mutagenesis system has enabled researchers to efficiently edit the clostridial genome. Since site-specific insertion of the...
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CRISPR/Cas9-assisted ssDNA recombineering for site-directed mutagenesis and saturation mutagenesis of plasmid-encoded genes
Site-directed and saturation mutagenesis are critical DNA methodologies for studying protein structure and function. For plasmid-based gene mutation,...
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Carbapenem-resistant hypervirulent ST23 Klebsiella pneumoniae with a highly transmissible dual-carbapenemase plasmid in Chile
BackgroundThe convergence of hypervirulence and carbapenem resistance in the bacterial pathogen Klebsiella pneumoniae represents a critical global...
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Complete sequence verification of plasmid DNA using the Oxford Nanopore Technologies’ MinION device
BackgroundSequence verification is essential for plasmids used as critical reagents or therapeutic products. Typically, high-quality plasmid sequence...
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Genomic deletions in Aureobasidium pullulans by an AMA1 plasmid for gRNA and CRISPR/Cas9 expression
BackgroundAureobasidium pullulans is a generalist polyextremotolerant black yeast fungus. It tolerates temperatures below 0 °C or salt...
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Biofilms preserve the transmissibility of a multi-drug resistance plasmid
Self-transmissible multidrug resistance (MDR) plasmids are a major health concern because they can spread antibiotic resistance to pathogens. Even...
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Development of orthogonal aminoacyl-tRNA synthetase mutant for incorporating a non-canonical amino acid
Genetic code expansion involves introducing non-canonical amino acids (ncAAs) with unique functional groups into proteins to broaden their...
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HAP40 modulates mutant Huntingtin aggregation and toxicity in Huntington’s disease mice
Huntington’s disease (HD) is a monogenic neurodegenerative disease, caused by the CAG trinucleotide repeat expansion in exon 1 of the Huntingtin...
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Optimization of linear plasmid expression system for protein production and secretion in Bacillus thuringiensis
Bacillus thuringiensis , a safe bacterium widely used in agriculture for the biocontrol of pests, has great potential for protein production. The...
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A pan-KRAS degrader for the treatment of KRAS-mutant cancers
KRAS mutations are highly prevalent in a wide range of lethal cancers, and these mutant forms of KRAS play a crucial role in driving cancer...
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Biofilms can act as plasmid reserves in the absence of plasmid specific selection
Plasmids facilitate rapid bacterial adaptation by shuttling a wide variety of beneficial traits across microbial communities. However, under...
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Development of Mutant Forms of Neuroglobin with Substitutions in the Interaction Surface with Cytochrome c
AbstractMutant forms of human neuroglobin that carry targeted mutations in the putative interface with cytochrome c : single (E60K, K67E, E87K, K95E)...
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Improved site-specific mutagenesis in Rhodococcus opacus using a novel conditional suicide plasmid
AbstractRhodococcus opacus PD630 is a biotechnologically important bacterium with metabolic capability for bioremediation, metal recovery, and...
