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High-Resolution Melting (HRM) Genoty**
High-resolution melting (HRM) analysis is a simple, fast, and inexpensive real-time polymerase chain reaction (PCR)-based method used to identify...
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High-Resolution Melting (HRM) analysis of DNA methylation using semiconductor chip-based digital PCR
BackgroundDigital PCR (dPCR) technology allows absolute quantification and detection of disease-associated rare variants, and thus the use of dPCR...
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Herbal species authentication by melting fingerprint coupled with high resolution melting analysis (MF-HRM)
BackgroundHerbal medicines have recently attracted increasing attention for use as food supplements with health benefits; however, species...
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Development of the genoty** marker for Reeves’ turtle (Mauremys reevesii) using high-resolution melting (HRM) analysis
The Reeves’ turtle, Mauremys reevesii , resides in freshwater bodies of Korea, China, Japan, and Taiwan, and is designated as an endangered species in...
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Modified High-Resolution Melting (HRM) Marker Systems Increasing Discriminability Between Homozygous Alleles
Targeted single-nucleotide polymorphism (SNP) genoty**, especially for functional nucleotide polymorphism, is widely used for current breeding...
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Distinguishing Palm White Grub Complex, Leucopholis spp. (Coleoptera: Scarabaeidae: Melolonthinae) From India Using High-Resolution Melting (HRM) Analyses
This study aimed to identify the differences among three species of palm white grubs at morphological, ethological, ecological and molecular levels...
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Application of a new designed high resolution melting analysis for mycobacterial species identification
The Non-tuberculous mycobacterial (NTM) isolates should be distinguished from tuberculosis and identified at the species level for choosing an...
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A method for identifying nine tuna and tuna-like species (tribe Thunnini) by using high-resolution melting analysis based on genoty** single nucleotide polymorphisms in mitochondrial DNA
Tuna and tuna-like fishes (tribe Thunnini) are pelagic species that are important worldwide for their ecological and economic value. Understanding...
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High Resolution Melt (HRM) Genoty** for Detection of Induced Mutations in Coffee (Coffea arabica L. var. Catuaí)
Arabica coffee (C. arabica L.) is a highly valued agricultural commodity on the world market. Tons of products are traded internationally, and it has...
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Assemblage characterization of Giardia duodenalis in South Khorasan province, eastern Iran, using HRM real-time PCR method
BackgroundGiardia duodenalis is a common parasitic protozoan causing gastrointestinal illness in humans worldwide. The genetic diversity of G....
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Rapid Identification of Four Fusarium spp. Complex by High-Resolution Melting Curve Analysis and their Antifungal Susceptibility Profiles
Fusarium species are globally distributed filamentous ascomycete fungi that are frequently reported as plant pathogens and opportunistic human...
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Association between the ELAVL1 gene single nucleotide polymorphisms and the Genetic Susceptibility to cervical cancer by high resolution melting in a Tunisian population
BackgroundHuman papillomavirus is the major cause of cervical cancer, but only few cases develop into cancer. Nevertheless, HuR (ELAVL1) gene has...
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Detection of Isoniazid and Rifampin Resistance in Mycobacterium tuberculosis Clinical Isolates from Sputum Samples by High-Resolution Melting Analysis
The effective management of multidrug-resistant tuberculosis (MDR-TB) and the need for rapid and accurate screening of rifampin (RIF) and isoniazid...
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High-resolution (mtDNA) melting analysis for simple and efficient characterization of Africanized honey bee Apis mellifera (Hymenoptera:Apidae)
Analysis of the mtDNA variation in Apis mellifera L. has allowed distinguishing subspecies and evolutionary lineages by means of different molecular...
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Direct Detection of Fluoroquinolone Resistance in Sputum Samples from Tuberculosis Patients by High Resolution Melt Curve Analysis
Multidrug-resistant tuberculosis (MDR-TB) requires treatment with fluoroquinolone (FLQ) drugs, however, the excessive use of FLQ has led to the rise...
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Multiplex detection of five common respiratory pathogens from bronchoalveolar lavages using high resolution melting curve analysis
BackgroundThe study describes the application of the multiplex high-resolution melting curve (MHRM) assay for the simultaneous detection of five...
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MS-HRM protocol: a simple and low-cost approach for technical validation of next-generation methylation sequencing data
DNA methylation is a fundamental epigenetic process and have a critical role in many biological processes. The study of DNA methylation at a large...
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Comparison of PCR-HRM, colorimetric LAMP and culture based diagnostic assays in the detection of endometritis caused by Streptococcus equi subsp. zooepidemicus in mares
Streptococcus equi subsp. zooepidemicus ( S. zooepidemicus ) is one of the causative agents of equine endometritis. In this study, a panel of different...
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Development of high-resolution melting PCR (HRM-PCR) assay to identify native fungal species associated with the wheat endosphere
Understanding the complexity and biodiversity of fungal communities associated with the wheat endosphere can facilitate the identification of novel...
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SNP genoty** and diversity analysis based on genic-SNPs through high resolution melting (HRM) analysis in blackgram [Vigna mungo (L.) Hepper]
Genetic relatedness among 27 blackgram [ Vigna mungo (L.) Hepper] genotypes was analysed using 19 genic-single nucleotide polymorphisms (SNPs) through...
