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Assemblage characterization of Giardia duodenalis in South Khorasan province, eastern Iran, using HRM real-time PCR method

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Abstract

Background

Giardia duodenalis is a common parasitic protozoan causing gastrointestinal illness in humans worldwide. The genetic diversity of G. duodenalis is reflected through the identification of different assemblages. In this study, we aimed to determine the assemblages of G. duodenalis in eastern Iran using nested-PCR and high-resolution melting (HRM) real-time PCR methods.

Methods

A total of 58 positive G. duodenalis, which were isolated from 1800 subjects, referred to medical center laboratories in South Khorasan province, eastern Iran, from April 2020 to March 2022, were included in this study. DNA was extracted and HRM real-time PCR was performed for assemblage characterization.

Results

HRM real-time PCR successfully characterized all samples. Accordingly, out of 58 positive samples, 53 (91.36%) and 5 (8.62%) were identified as assemblage A and B, respectively.

Conclusions

Our findings showed that HRM real-time PCR was able to characterize the assemblages of G. duodenalis. In addition, our results suggest high prevalence of assemblage A in eastern region of Iran.

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Data availability

All generated data from the current study are included in the article.

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Acknowledgements

The present manuscript was a component of Mahmoodreza Behravan’s PhD thesis and Moloud Tabrizi’s MSc thesis. This research work received financial support from the Deputy Research Office of the School of Medicine at Shahid Beheshti University of Medical Sciences. We would like to take this opportunity to express our sincere gratitude for the cooperation and support provided by all personnel of the health centers in South Khorasan Province, Iran and Foodborne and Waterborne Diseases Research Center, Research Institute for Gastroenterology and Liver, Shahid Beheshti University of Medical Sciences, Iran.

Funding

This study received support from the Research Deputy of the School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran (Grant Numbers: 25680 and 33013).

Author information

Authors and Affiliations

  1. Department of Medical Parasitology and Mycology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran

    Moloud Tabrizi, Seyyed Javad Seyyed Tabaei, Zohreh Lasjerdi & Ali Haghighi

  2. Infectious Diseases Research Center, Birjand University of Medical Sciences, Birjand, Iran

    Mahmoodreza Behravan

  3. Foodborne and Waterborne Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran

    Hanieh Mohammad Rahimi & Hamed Mirjalali

  4. Basic and Molecular Epidemiology of Gastrointestinal Disorders Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran

    Mohammad Amin Pourhoseingholi

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  1. Moloud Tabrizi
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Contributions

Conceived and designed: AH, HM. Sample collection and questionnaire: MB MT. Methodology and implementation: MT, ZL, HMR, SJST. Statistical analysis: MAP. Reviewing and editing the manuscript: AH, HM. All authors read and approved the final version of the manuscript.

Corresponding authors

Correspondence to Hamed Mirjalali or Ali Haghighi.

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Conflict of interest

The author declares that they have no conflict of interests.

Ethical approval

All study participants completed a written informed consent before specimen collection. The study was approved by the Ethics Committee of Shahid Beheshti University of Medical Sciences, Tehran, Iran (IR.SBMU.MSP.REC.1399.754) and (IR.SBMU.MSP.REC.1401.272).

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Informed consent was obtained from all subjects and/or their legal guardian(s). All authors declare that they have seen and approved the submitted version of this manuscript.

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Tabrizi, M., Behravan, M., Seyyed Tabaei, S.J. et al. Assemblage characterization of Giardia duodenalis in South Khorasan province, eastern Iran, using HRM real-time PCR method. Mol Biol Rep 51, 127 (2024). https://doi.org/10.1007/s11033-023-09001-3

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