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Assemblage characterization of Giardia duodenalis in South Khorasan province, eastern Iran, using HRM real-time PCR method
BackgroundGiardia duodenalis is a common parasitic protozoan causing gastrointestinal illness in humans worldwide. The genetic diversity of G....
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High-Resolution Melting (HRM) analysis of DNA methylation using semiconductor chip-based digital PCR
BackgroundDigital PCR (dPCR) technology allows absolute quantification and detection of disease-associated rare variants, and thus the use of dPCR...
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High-Resolution Melting (HRM) Genoty**
High-resolution melting (HRM) analysis is a simple, fast, and inexpensive real-time polymerase chain reaction (PCR)-based method used to identify...
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Herbal species authentication by melting fingerprint coupled with high resolution melting analysis (MF-HRM)
BackgroundHerbal medicines have recently attracted increasing attention for use as food supplements with health benefits; however, species...
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Rapid detection of tomato spotted wilt virus by real-time RT-LAMP and in-field application
Tomato spotted wilt virus (TSWV) is considered one of the most threatening viruses worldwide for different economically important agricultural crops....
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Development of the genoty** marker for Reeves’ turtle (Mauremys reevesii) using high-resolution melting (HRM) analysis
The Reeves’ turtle, Mauremys reevesii , resides in freshwater bodies of Korea, China, Japan, and Taiwan, and is designated as an endangered species in...
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Evaluation of filter paper to transport inactivated bacteria to detect carbapenem resistance genes by multiplex real-time PCR using high-resolution melting
Infections caused by resistant microorganisms are a complex global public health challenge, and the way to combat the increase of resistance is the...
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Distinguishing Palm White Grub Complex, Leucopholis spp. (Coleoptera: Scarabaeidae: Melolonthinae) From India Using High-Resolution Melting (HRM) Analyses
This study aimed to identify the differences among three species of palm white grubs at morphological, ethological, ecological and molecular levels...
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High Resolution Melt (HRM) Genoty** for Detection of Induced Mutations in Coffee (Coffea arabica L. var. Catuaí)
Arabica coffee (C. arabica L.) is a highly valued agricultural commodity on the world market. Tons of products are traded internationally, and it has...
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Specific Detection of Pseudomonas savastanoi Pathovars: From End-Point PCR to Real-Time PCR and HRMA
Pseudomonas savastanoi is a phytopathogenic bacterium causing severe disease on olive, oleander, ash, and other Oleaceae. Three main pathovars belong...
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Development of multiplex HRM-based loop-mediated isothermal amplification method for specific and sensitive detection of Treponema pallidum
Syphilis is a sexually transmitted disease caused by the spirochaete bacterium Treponema pallidum . This study has developed a multiplex...
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BRCA1 and BRCA2 screening of nine Chilean founder mutations through allelic-discrimination and real-time PCR in breast/ovarian cancer patients
BackgroundIn a previous work, we identified nine founder mutations present in close to 80% of BRCA1 and BRCA2 mutation carriers, and distributed...
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Development and performance evaluation of TaqMan real-time fluorescence quantitative methylation specific PCR for detecting methylation level of PER2
BackgroundPER2 gene methylation is closely related to the occurrence and progress of some cancers, but there is no method to quantitatively detect...
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TaqMan real time PCR for the Detection of the Gilbert’s Syndrome Markers UGT1A1*28; UGT1A1*36 and UGT1A1*37
Gilbert’s syndrome is characterized by mild unconjugated hyperbilirubinemia. The key of this disease is a diminished activity of...
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QTL analysis and development of HRM markers associated with fruit shape in interspecific pears (Pyrus pyrifolia × P. bretschneideri)
Fruit shape is one of the important quantitative traits in pear ( Pyrus spp.) breeding program, thus genetic study related to fruit shape could be...
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Development of high-resolution melting PCR (HRM-PCR) assay to identify native fungal species associated with the wheat endosphere
Understanding the complexity and biodiversity of fungal communities associated with the wheat endosphere can facilitate the identification of novel...
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Application of a new designed high resolution melting analysis for mycobacterial species identification
The Non-tuberculous mycobacterial (NTM) isolates should be distinguished from tuberculosis and identified at the species level for choosing an...
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A method for identifying nine tuna and tuna-like species (tribe Thunnini) by using high-resolution melting analysis based on genoty** single nucleotide polymorphisms in mitochondrial DNA
Tuna and tuna-like fishes (tribe Thunnini) are pelagic species that are important worldwide for their ecological and economic value. Understanding...
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Machine learning based DNA melt curve profiling enables automated novel genotype detection
Surveillance for genetic variation of microbial pathogens, both within and among species, plays an important role in informing research, diagnostic,...
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Use of Advanced Molecular Techniques for Human Body Fluids Detection
Forensic body fluid analysis is being modernized by the application of advanced molecular techniques for human body fluids identification. Detection...