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Assessment of reference genes for qRT-PCR normalization to elucidate host response to African swine fever infection
Viral infection disrupts the normal regulation of the host gene’s expression. In order to normalise the expression of dysregulated host genes upon...
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Selection and validation of reference genes for the normalization of quantitative real-time PCR in different muscle tissues of rabbits
BackgroundIn molecular biology studies, the selection of optimal reference genes is of vital importance for accurately quantifying gene expression....
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Purine salvage–associated metabolites as biomarkers for early diagnosis of esophageal squamous cell carcinoma: a diagnostic model–based study
Esophageal squamous cell carcinoma (ESCC) remains an important health concern in develo** countries. Patients with advanced ESCC have a poor...
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Selection of reference genes in liproxstatin-1-treated K562 Leukemia cells via RT-qPCR and RNA sequencing
BackgroundReverse transcription quantitative polymerase chain reaction (RT-qPCR) can accurately detect relative gene expression levels in biological...
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Identification and validation of stable reference genes for quantitative real time PCR in different minipig tissues at developmental stages
BackgroundQuantitative real time PCR (qPCR) is a powerful tool to evaluate mRNA expression level. However, reliable qPCR results require...
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Comprehensive evaluation and validation of optimal reference genes for normalization of qPCR data in different caprine tissues
BackgroundQuantitative real-time PCR (qPCR) is a highly reliable method for validating gene expression data in molecular studies due to its...
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DNA polymerases in precise and predictable CRISPR/Cas9-mediated chromosomal rearrangements
BackgroundRecent studies have shown that, owning to its cohesive cleavage, Cas9-mediated CRISPR gene editing outcomes at junctions of chromosomal...
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Epigenetic-related transcriptional reprogramming elucidated by identification and validation of a novel reference gene combination for RT-qPCR studies in porcine oocytes of contrasting quality
BackgroundReliable RT-qPCR results are dependent on appropriate normalisation. Oocyte maturation studies can be challenging in this respect, as the...
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Screening and stability analysis of reference genes in fasting caecotrophy model in rabbits
BackgroundThe selection and validation of stably expressed reference genes is key for accurately quantifying the mRNA abundance of genes under...
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The Reference Gene Validation in the Brain of Rats during Antioxidant and Anti-Inflammatory Treatment in the Lithium-Pilocarpine Model of Temporal Epilepsy
AbstractReverse transcription combined with quantitative (real-time) polymerase chain reaction (RT-qPCR) is the gold standard for assessing gene...
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The impact of pre-freezing storage time and temperature on gene expression of blood collected in EDTA tubes
BackgroundBlood is a common source of RNA for gene expression studies. However, it is known to be vulnerable to pre-analytical variables. Although...
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Screening of reference genes in tiger puffer (Takifugu rubripes) across tissues and under different nutritional conditions
The present study was aimed at screening suitable reference genes for quantitative real-time polymerase chain reaction (qRT-PCR) in tiger puffer ( Taki...
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Selection of reference genes for quantitative real-time PCR normalization in European quail tissues
Coturniculture has been standing out as an industrial poultry activity in several countries around the world because of the several adaptive...
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Hybridoma Technology
Monoclonal antibodies are produced by hybridoma technology. The term hybridoma is applied to fused cells resulting due to fusion of lymphocytes and...
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Precise and Scarless Insertion of Transposable Elements by Cas9-Mediated Genome Engineering
Transposable element insertions can have broad effects on gene expression, ranging from new regulatory functions to pathogenic consequences by...
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Efficient prioritization of CRISPR screen hits by accounting for targeting efficiency of guide RNA
BackgroundCRISPR-based screens are revolutionizing drug discovery as tools to identify genes whose ablation induces a phenotype of interest. For...
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Expression analysis of proteins and genes related to phagosome-lysosome and immunohistochemical features of Cynoglossus semilaevis upon Vibrio vulnificus infection
The phagosome-lysosome (PL) signaling pathway plays an important role in the immune system of fish. However, the molecular mechanism underlying PL...
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The Elk-3 target Abhd10 ameliorates hepatotoxic injury and fibrosis in alcoholic liver disease
Alcoholic liver disease (ALD) and other forms of chronic hepatotoxic injury can lead to transforming growth factor β1 (TGFβ1)-induced hepatic...
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ExonSurfer: a web-tool to design primers at exon–exon junctions
BackgroundReverse transcription quantitative PCR (RT-qPCR) with intercalating dyes is one of the main techniques to assess gene expression levels...
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Osmolarity modulates the de-differentiation of horse articular chondrocytes during cell expansion in vitro: implications for tissue engineering in cartilage repair
Due to the importance of joint disease and ostearthritis (OA) in equine athletes, new regenerative treatments to improve articular cartilage repair...
