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Expression analysis of proteins and genes related to phagosome-lysosome and immunohistochemical features of Cynoglossus semilaevis upon Vibrio vulnificus infection

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Abstract

The phagosome-lysosome (PL) signaling pathway plays an important role in the immune system of fish. However, the molecular mechanism underlying PL signaling in fish against Vibrio vulnificus infection remains still unclear. In this study, the half-smooth tongue sole (Cynoglossus semilaevis) infected with V. vulnificus strain ST-6 was used as an experiment model, the immunohistochemical features were evaluated, and spatiotemporal expression analysis of genes and proteins related to PL pathway was verified, respectively. The results showed that the positive staining was observed in the various tissues such as liver, intestine, kidney, spleen, and gills of the infected fish. The differential expressions of PL-related proteins were screened and identified by iTRAQ analysis, and their gene expressions were determined by qRT-PCR detection. The spatiotemporal expression patters were confirmed and most of PL-related genes tended to be downregulated during the early infection at 12 and 36 h post-infection (hpi). Moreover, the protein-protein interaction (PPI) analysis was described for PL pathway. This is the first report on the PL response of C. semilaevis to V. vulnificus infection, which will provide a scientific reference for further elucidating the molecular mechanism of the phagosome-lysosome in marine fish.

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Funding

This study was supported by the National Natural Science Foundation of China (no. 32273182), the Teaching Program of Tian** Agricultural University (2021-A-01), and the Natural Science Foundation of Tian** City of China (no. 23JCZDJC00240).

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  1. Hao Fu and Minghao Ye contributed equally to this work.

Authors and Affiliations

  1. College of Fisheries, Tian** Agricultural University, Tian**, 300392, China

    Hao Fu, Minghao Ye, **ucai Hu & Aijun Lv

  2. Tian** Key Lab of Aqua-Ecology and Aquaculture, Tian** Agricultural University, Tian**, 300392, China

    **ucai Hu & Aijun Lv

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  1. Hao Fu
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  2. Minghao Ye
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  3. **ucai Hu
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Contributions

Hao Fu: writing—original draft; software; formal analysis; methodology. Minghao Ye: data curation, software, validation, formal analysis, methodology. **ucai Hu: writing—original draft; methodology; formal analysis; supervision. Aijun Lv: supervision, review and editing, funding acquisition.

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Correspondence to **ucai Hu or Aijun Lv.

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All experimental animal protocols were carried out according to the guidelines of the Animal Ethics Committee and were approved by the Tian** Agriculture University Institutional Animal Care and Use Committee (TAJU-IACUC).

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Fu, H., Ye, M., Hu, X. et al. Expression analysis of proteins and genes related to phagosome-lysosome and immunohistochemical features of Cynoglossus semilaevis upon Vibrio vulnificus infection. Aquacult Int (2024). https://doi.org/10.1007/s10499-024-01577-2

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