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Enzyme Identification and Screening: Activity-Based Methods
Methods for the identification and characterization of “ideal” enzymes for industrial use in the field of biotechnology have developed rapidly in...
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Assaying Lysosomal Enzyme Activity in Dictyostelium discoideum
Lysosomes are membrane-enclosed organelles that digest intracellular material. They contain more than 50 different enzymes that can degrade a variety...
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The Protection of Enzyme Activity for the Preparation of Humanized Polymerized Hemoglobin-Superoxide Dismutase-Catalase-Carbonic Anhydrase
AbstractThis work finds suitable enzyme activity protectants to improve the recovery rate of enzyme activity in the preparation of human polymerized...
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Fluorometric Characterization of DUB Activity: From Single-Enzyme Reactions to Live Intact Cells
Fluorescently tagged molecular probes capable of time- and concentration-dependent quantification of deubiquitinating enzyme (DUB) activity allow for...
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Enzyme Production
The choice of a host organism is crucial for enzyme production. We provide an overview of the limiting factors in protein biosynthesis that influence...
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Enzyme Kinetics
In this chapter, you will learn the fundamental concepts of chemical and enzyme catalysis as well as kinetics such as the lock and key and the...
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Enzyme Activity Control and Protein Conformational Change
Controlling the enzyme activity shows potential for significant contributions to the pre−/postharvest food industry. In this chapter, the...
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Environmental activity-based protein profiling for function-driven enzyme discovery from natural communities
BackgroundMicrobial communities are important drivers of global biogeochemical cycles, xenobiotic detoxification, as well as organic matter...
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Extracellular Enzyme Activity and Stoichiometry Across Vegetated and Non-Vegetated Coastal Ecosystems
The conversion of organic matter by extracellular enzymes can reveal important insights into carbon and nutrient cycling. The activity and...
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Enzyme Immobilization
Immobilization is a comparatively old approach to make enzymes technically usable. The aims are both to increase productivity and enzyme lifetimes...
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Conservation tillage increases nutrient accumulation by promoting soil enzyme activity: a meta-analysis
AimsThe conservation tillage i.e. reduced tillage (incl. no tillage and minimum tillage) and straw return, plays an important role in the promotion...
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Enzyme Purification
The growing importance of enzymes for the food, biotech, pharmaceutical, and cosmetics industries, among others, has increased the need for efficient...
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Phosphorus fertilization affects litter quality and enzyme activity in a semiarid grassland
AimsLitter decomposition affects soil organic carbon storage, and nutrient addition alters exogenous nutrient availability in soil, thereby affecting...
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Map** enzyme activity in living systems by real-time mid-infrared photothermal imaging of nitrile chameleons
Simultaneous spatial map** of the activity of multiple enzymes in a living system can elucidate their functions in health and disease. However,...
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DNA methylases for site-selective inhibition of type IIS restriction enzyme activity
AbstractDNA methylases of the restriction-modifications (R-M) systems are promising enzymes for the development of novel molecular and synthetic...
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Non-Catalytic Domains of DNA Polymerase λ: Influence on Enzyme Activity and Its Regulation
AbstractDNA polymerase λ (Polλ) belongs to the same structural X-family as DNA polymerase β, the main polymerase of base excision repair. The role of...
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Effect of Light Intensity on Blueberry Fruit Coloration, Anthocyanin Synthesis Pathway Enzyme Activity, and Gene Expression
AbstractThe fruit skin color is an important economic trait of blueberries ( Vaccinium spp.) and is determined by the concentration of anthocyanins....
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Physiological Response and Antioxidant Enzyme Activity of New Durum Wheat Varieties under Heat Stress
AbstractHeat stress is one of the most important environmental factors causing serious problems to durum wheat production in arid and semi-arid...
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Soil Enzyme Activity in Natural and Plowed Catenas as Parameters of Physiological State of Microbial Communities
AbstractThe study of soil microbial biomass and enzymatic activity of natural and anthropogenically transformed ecosystems was carried out. The...
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Enzyme Structure and Function
This chapter provides an overview on the general architecture of enzymes, describes protein structure and the types of enzyme cofactors, and...