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Establishment of a rapid assay for sequencing of carried DNA and edited sites in gene-editing tomato plants
Gene editing can modify gene function by inserting or deleting nucleotides in a specific region in a gene, which can alter the desired trait in the...
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DNA Sequencing Technologies and DNA Barcoding
DNA barcodes are short, standardized DNA segments that geneticists can use to identify all living taxa. On the other hand, DNA barcoding identifies...
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A Rapid, Equipment-Free DNA Isolation Method for DNA Barcoding
This rapid, equipment-free DNA isolation procedure using chromatography paper is a simple method that can be performed in less than 30 min and...
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Chromatin Modifications in DNA Repair
A requirement of nuclear processes that use DNA as a substrate is the manipulation of chromatin in which the DNA is packaged. Chromatin...
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Community Science Initiatives Utilizing Environmental DNA
The technology that utilizes environmental DNA (eDNA), which refers to DNA released by organisms into the environment, has rapidly advanced as a...
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ddRAD Sequencing and DNA Barcoding
Double-digest restriction site-associated DNA sequencing is a library preparation protocol that enables capturing variable sites across the genome...
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Simple, Robust Invertebrate DNA Barcoding: Chelex-Based DNA Extraction and Optimized COI Amplification
Chelex-based DNA extractions are well suited for student DNA barcoding research because they are simple, safe, and inexpensive and can be performed...
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Classical Recombinant DNA Cloning
Traditional molecular cloning involves a series of linked experimental steps performed with the overall goal of isolating (“cloning”) a specific DNA...
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iTaxoTools 1.0: Improved DNA Barcode Exploration with TaxI2
The overall availability of user-friendly software tools tailored to the analysis of DNA barcodes is limited. Several obvious functions such as...
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Single Molecule Imaging of DNA–Protein Interactions Using DNA Curtains
Direct observation of enzymes that work to promote nucleic acid metabolism is a powerful approach to understanding their biochemical and biological...
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DNA as Hereditary Material
The relative simplicity of the structure of deoxyribonucleic acid (DNA) and the complexity of inheritance led to the assumption that DNA was not the...
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Small RNA and DNA Methylation of Tea Plants
Small RNAs are a category of short noncoding RNAs (ncRNAs) with 18–30 nt lengths. Based on their function and mechanisms of biogenesis, these small...
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DNA Probes for Analysis of the Activity of Key Enzymes of the Base Excision DNA Repair Pathway in Human Cells
Abstract —The important role of DNA damage in the occurrence of various diseases, including cancer, has led to study of the mechanisms of genetic...
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Influence of Nucleotide Context on Non-Specific Amplification of DNA with Bst exo– DNA Polymerase
AbstractIsothermal nucleic acids amplification that requires DNA polymerases with strand-displacement activity gained more attention in the last two...
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Manual Silica-Based DNA Extractions
There are several silica-based extraction methods that utilize silica-packed columns or silica-coated paramagnetic resin and are suitable for the...
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DNA Barcoding for Assessing Biodiversity
DNA barcoding is a powerful tool to identify species using nucleotide differences found in the cytochrome oxidase I mitochondrial gene between...
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Analysis of Ancient Microbial DNA
The development of next-generation sequencing has led to a breakthrough in the analysis of ancient genomes, and the subsequent genomic analyses of...
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Future Directions of Forensic DNA Analysis
Since its inception in mid 1980s, forensic DNA analysis techniques have progressed much in last few decades. The first decade (1985–1995) is...
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The Efficiency of Various DNA Polymerases for Amplification of Long Sequences from Genomic DNA and cDNA of Cultivated Potatoes
AbstractAmplification of long fragments from complex templates, such as eukaryotic genomic DNA, is considered a difficult task for most DNA...
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DNA double-strand break–capturing nuclear envelope tubules drive DNA repair
Current models suggest that DNA double-strand breaks (DSBs) can move to the nuclear periphery for repair. It is unclear to what extent human DSBs...
