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  1. Modifying Bacterial Artificial Chromosomes for Extended Genome Modification

    Bacterial artificial chromosomes have been used extensively for the exploration of mammalian genomes. Although novel approaches made their initial...
    Hannah Auch, Nikolai Klymiuk, Petra Runa-Vochozkova in Applications of Genome Modulation and Editing
    Protocol 2022
  2. Transcription-induced domains form the elementary constraining building blocks of bacterial chromosomes

    Transcription generates local topological and mechanical constraints on the DNA fiber, leading to the generation of supercoiled chromosome domains in...

    Amaury Bignaud, Charlotte Cockram, ... Romain Koszul in Nature Structural & Molecular Biology
    Article Open access 04 January 2024
  3. Dynamics of Bacterial Chromosomes by Locus Tracking in Fluorescence Microscopy

    In the last two decades, it has been shown that bacterial chromosomes have remarkable spatial organization at various scales, and they display...
    Leonardo Mancini, Estelle Crozat, ... Pietro Cicuta in Chromosome Architecture
    Protocol 2022
  4. Plant Cytogenetics: From Chromosomes to Cytogenomics

    Chromosomes have been studied since the late nineteenth century in the disciplines of cytology and cytogenetics. Analyzing their numbers, features,...
    Trude Schwarzacher, Qing Liu, J. S. (Pat) Heslop-Harrison in Plant Cytogenetics and Cytogenomics
    Protocol 2023
  5. Capability of a large bacterial artificial chromosome clone harboring multiple biosynthetic gene clusters for the production of diverse compounds

    The biosynthetic gene clusters (BGCs) for the macrocyclic lactone-based polyketide compounds are extremely large-sized because the polyketide...

    Kei Kudo, Takehiro Nishimura, ... Kazuo Shin-ya in The Journal of Antibiotics
    Article 04 March 2024
  6. Flow Cytometric Analysis and Sorting of Plant Chromosomes

    Flow cytometry offers a unique way of analyzing and manipulating plant chromosomes. During a rapid movement in a liquid stream, large populations can...
    Petr Cápal, Mahmoud Said, ... Jaroslav Doležel in Plant Cytogenetics and Cytogenomics
    Protocol 2023
  7. Machine learning-aided scoring of synthesis difficulties for designer chromosomes

    Designer chromosomes are artificially synthesized chromosomes. Nowadays, these chromosomes have numerous applications ranging from medical research...

    Yan Zheng, Kai Song, ... Ying-** Yuan in Science China Life Sciences
    Article 03 March 2023
  8. Flow cytometric analysis and sorting of plant chromosomes

    Flow cytogenetics is a high-throughput technique that classifies large populations of mitotic chromosomes according to their fluorescence and light...

    Jaroslav Doležel, Petr Urbiš, ... István Molnár in The Nucleus
    Article Open access 16 November 2023
  9. Fungal and Bacterial Diseases of Wheat

    Wheat (Triticum aestivum L.) is the main staple crop and primary source of starch and energy in human diet. It is grown in over 120 countries in the...
    Hakan Hekimhan, Mehmet AydoÄźdu in Advances in Wheat Breeding
    Chapter 2024
  10. Molecular characterization of eliminated chromosomes in Hessian fly (Mayetiola destructor (Say))

    Like other cecidomyiid Diptera, Hessian fly has stable S chromosomes and dispensable E chromosomes that are retained only in the germ line. Amplified...

    Yan M. Crane, Charles F. Crane, ... Brandon J. Schemerhorn in Chromosome Research
    Article Open access 24 January 2023
  11. Fluorescence In Situ Hybridization of DNA Probes on Mitotic Chromosomes of the Mexican Axolotl

    Fluorescence in situ hybridization (FISH) is used extensively for visual localization of specific DNA fragments (and RNA fragments) in broad...
    Melissa Keinath, Vladimir Timoshevskiy in Salamanders
    Protocol 2023
  12. MDM2 amplification in rod-shaped chromosomes provides clues to early stages of circularized gene amplification in liposarcoma

    Well-differentiated liposarcoma (WDLS) displays amplification of genes on chromosome 12 (Chr12) in supernumerary ring or giant marker chromosomes....

    Saskia Sydow, Paul Piccinelli, ... Fredrik Mertens in Communications Biology
    Article Open access 20 May 2024
  13. Telomere-to-telomere assembly of diploid chromosomes with Verkko

    The Telomere-to-Telomere consortium recently assembled the first truly complete sequence of a human genome. To resolve the most complex repeats, this...

    Mikko Rautiainen, Sergey Nurk, ... Sergey Koren in Nature Biotechnology
    Article 16 February 2023
  14. Identification of new genetic sources of resistance to bacterial blight race 18 in diploid Asiatic cotton and resistance transfer to tetraploid Upland cotton (Gossypium hirsutum)

    Bacterial blight (BB) caused by Xanthomonas citri pv. malvacearum (Xcm), poses a significant threat to Upland cotton ( Gossypium hirsutum L.)...

    Abdelraheem Abdelraheem, Yi Zhu, ... **fa Zhang in Euphytica
    Article 09 May 2024
  15. Exogenous artificial DNA forms chromatin structure with active transcription in yeast

    Yeast artificial chromosomes (YACs) are important tools for sequencing, gene cloning, and transferring large quantities of genetic information....

    Jianting Zhou, Chao Zhang, ... Ying-** Yuan in Science China Life Sciences
    Article Open access 27 December 2021
  16. ProcaryaSV: structural variation detection pipeline for bacterial genomes using short-read sequencing

    Background

    Structural variations play an important role in bacterial genomes. They can mediate genome adaptation quickly in response to the external...

    Robin Jugas, Helena Vitkova in BMC Bioinformatics
    Article Open access 09 July 2024
  17. Meiotic pairing and double-strand break formation along the heteromorphic threespine stickleback sex chromosomes

    Double-strand break repair during meiosis is normally achieved using the homologous chromosome as a repair template. Heteromorphic sex chromosomes...

    Shivangi Nath, Lucille A. Welch, ... Michael A. White in Chromosome Research
    Article 30 May 2022
  18. Chromosome Painting Using Chromosome-Specific BAC Clones

    Chromosome painting (CP) refers to visualization of large chromosome regions, chromosome arms or entire chromosomes via fluorescence in situ...
    Terezie M. Mandáková, Martin A. Lysak in Plant Cytogenetics and Cytogenomics
    Protocol 2023
  19. Bacterial Endosymbionts of Mucoromycota Fungi: Diversity and Function of their Interactions

    Endosymbiotic bacteria are commonly inhabitants of environmental isolates of Mucoromycota fungi. Described bacterial endosymbionts of Mucoromycota...
    Jessie K. Uehling, Alessandra Salvioli, ... Paola Bonfante in Evolution of Fungi and Fungal-Like Organisms
    Chapter 2023
  20. Artificial genetic polymers against human pathologies

    Originally discovered by Nielsen in 1991, peptide nucleic acids and other artificial genetic polymers have gained a lot of interest from the...

    Gleb S. Ivanov, Vyacheslav G. Tribulovich, ... Nikolai A. Barlev in Biology Direct
    Article Open access 06 December 2022
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