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First report of whole genome sequence of septicemic Pasteurella multocida serovar B:2 ‘Soron’ strain isolated from swine
Pig pasteurellosis, caused by Pasteurella multocida , is an acute infection that also has economic implications for pig farmers. We report the...
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Characterization and phylogenetic analysis of the complete mitochondrial genome sequence of Auricularia villosula, an edible wild mushroom in China
Auricularia is a genus of edible jelly fungi whose highly plastic fruiting bodies lack differentiation between stalks and caps, making accurate...
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Sanger Sequencing to Determine the Full-Length Sequence of Circular RNAs
The pre-existing theory of pre-mRNA splicing into linear mature RNA was questioned with the introduction of circular RNAs (circRNAs). Hundreds of... -
Identification of single nucleotide polymorphism in StCWIN1 and development of Kompetitive Allele-Specific PCR (KASP) marker associated with tuber traits in potato
Crop yield and quality are significantly affected by carbon partitioning between the source and sink tissues and organs, a biological process that...
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Reference Genome Sequence of Flax
Flax (Linum usitatissimum L.) is an economically important fibre and oilseed crop with a relatively small genome size, estimated at 370–455 Mb,... -
The 29-nucleotide deletion in SARS-CoV: truncated versions of ORF8 are under purifying selection
BackgroundAccessory proteins have diverse roles in coronavirus pathobiology. One of them in SARS-CoV (the causative agent of the severe acute...
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Sequence Retrieval, Analysis, and Manipulation
In the early 1960s, when computers capable of analyzing were unavailable known protein sequences accumulated slowly. In this pre-computer era,... -
Nucleotide Biosynthesis and Regulation
Bacteria are fast-growing organisms that need to rapidly synthesize nucleotides to sustain growth. This is true for cancer cells as well. Thus,... -
Factors Affecting the Stability of the Trimer of 2'-Deoxyuridine 5'-Triphosphate Nucleotide Hydrolase from Escherichia coli
Abstract2′-Deoxyuridine 5′-triphosphate nucleotide hhydrolase (Dut) hydrolyzes dUTP to dUMP and pyrophosphate to prevent erroneous incorporation of...
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Semi-supervised learning improves regulatory sequence prediction with unlabeled sequences
MotivationGenome-wide association studies have systematically identified thousands of single nucleotide polymorphisms (SNPs) associated with complex...
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Entourage: all-in-one sequence analysis software for genome assembly, virus detection, virus discovery, and intrasample variation profiling
BackgroundPan-virus detection, and virome investigation in general, can be challenging, mainly due to the lack of universally conserved genetic...
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Isolation and characterization of 17 polymorphic microsatellite loci with tri- and tetra-nucleotide repeat motifs for Ayu (Plecoglossus altivelis) using next-generation sequencing
BackgroundAyu or sweetfish, Plecoglossus altivelis , an amphidromous fish ranging in the northwestern Pacific, is economically important inland...
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The effects of electromagnetic field radiation of extremely low frequency on growth parameters and nucleotide substitutions in L. minor clones
Current technologies have become a source of electromagnetic pollution resulting from artificially generated electromagnetic radiation (EMR). To...
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Novel and deleterious nucleotide variations in the HAND1 gene probably affect miRNA target sites and protein function in pediatric patients with congenital heart disease
BackgroundCongenital heart disease (CHD) is the most prevalent developmental defect and principal cause of infant mortality and affects cardiac and...
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The complete sequence of Lens tomentosus chloroplast genome
A self-pollinated, annual, and nutrient-rich legume, Lens tomentosus has excellent economic value since seeds of L. tomentosus are used all over the...
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Rapid and accurate taxonomic classification of cpn60 amplicon sequence variants
The “universal target” region of the gene encoding the 60 kDa chaperonin protein (cpn60, also known as groEL or hsp60) is a proven sequence barcode...
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CRISPR–Cas9 bends and twists DNA to read its sequence
In bacterial defense and genome editing applications, the CRISPR-associated protein Cas9 searches millions of DNA base pairs to locate a...
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Single-Nucleotide Polymorphism
In forensic analysis, the conventional short tandem repeat (STR) markers are routinely used for examination of the biological samples. But, in the... -
Essential genes identification model based on sequence feature map and graph convolutional neural network
BackgroundEssential genes encode functions that play a vital role in the life activities of organisms, encompassing growth, development, immune...
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SALON ontology for the formal description of sequence alignments
BackgroundInformation provided by high-throughput sequencing platforms allows the collection of content-rich data about biological sequences and...