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DNA methylases for site-selective inhibition of type IIS restriction enzyme activity
AbstractDNA methylases of the restriction-modifications (R-M) systems are promising enzymes for the development of novel molecular and synthetic...
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Melanin decolorization by lysosome-related extract in Saccharomyces cerevisiae modified to overproduce glutathione peroxidase
AbstractAll eukaryotes have lysosomes that contain hydrolytic enzymes, such as protease, that degrade waste materials and cellular fragments. As a...
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Insect Cells-Baculovirus System for the Production of Difficult to Express Proteins: From Expression Screening for Soluble Constructs to Protein Quality Control
Rapid preparation of proteins for functional and structural analysis is a major challenge both in academia and industry. The number potential targets...
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Mechanochemical Evolution of Disulfide Bonds in Proteins
Disulfide bonds play a pivotal role in the mechanical stability of proteins. Numerous proteins that are known to be exposed to mechanical forces in...
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Generation of Mouse Model (KI and CKO) via Easi-CRISPR
Recent development of Easi-CRISPR (Efficient additions with ssDNA inserts-CRISPR) that utilizes long single-stranded DNA (lssDNA) of 0.2–2 kbases in...
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FRET Analysis of RNA –Protein Interactions Using Spinach Aptamers
The method developmentRibonucleic acid (RNA) to analyze direct RNA–protein interaction is of high importance as not many homogeneous assay formats...
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Identification and characterization of a novel lytic peptidoglycan transglycosylase (MltC) in Shigella dysenteriae
Shigellosis remains a worldwide health problem due to the lack of vaccines and the emergence of antibiotic-resistant strains. Shigella (S.) dysenteriae ...
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Targeted Gene Disruption Via CRISPR/Cas9 Ribonucleoprotein Complexes in Fusarium oxysporum
Characterization of a gene of interest frequently relies on generation of a mutant as a critical component. Transformation to disrupt a gene has been...
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Single Base Editing Using Cytidine Deaminase to Change Grain Size and Seed Coat Color in Rice
The fast-moving CRISPR technology has allowed plant scientists to manipulate plant genomes in a targeted manner. So far, most of the applications...
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Generation and maintenance of the circularized multimeric IS26-associated translocatable unit encoding multidrug resistance
In gram-negative bacteria, IS 26 often exists in multidrug resistance (MDR) regions, forming a pseudocompound transposon (PCTn) that can be tandemly...
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Generation of Knock-In Mouse by Genome Editing
Knock-in mice are useful for evaluating endogenous gene expressions and functions in vivo. Instead of the conventional gene-targeting method using...
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Expression of the GFP-mammalian pleckstrin homology (PH) domain of the phospholipase C δ1 in Saccharomyces cerevisiae BY4741
BackgroundPleckstrin homology (PH) domains are common modules of ∼120 amino acids found in proteins involved in signalling, cytoskeletal...
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Expression and characterization of heparinase II with MBP tag from a novel strain, Raoultella NX-TZ-3-15
The enzymes are biological macromolecules that biocatalyze certain biochemical reactions without undergoing any modification or degradation at the...
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Single-Molecule RNA Imaging in Live Cells with an Avidity-Based Fluorescent Light-Up Aptamer biRhoBAST
Observing individual RNA molecules provides valuable insights into their regulation, interactions with other cellular components, organization, and...
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Production of Multiprotein Complexes Using the Baculovirus Expression System: Homology-Based and Restriction-Free Cloning Strategies for Construct Design
Most cellular processes are mediated by multi-subunit protein complexes which have attracted major interest in both academia and industry....
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Measurements of Real-Time Replication Kinetics of DNA Polymerases on ssDNA Templates Coated with Single-Stranded DNA-Binding Proteins
Optical tweezers can monitor and control the activity of individual DNA polymerase molecules in real time, providing in this way unprecedented...
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Generating Clonal Seeds from Hybrid Rice with CRISPR-Cas9
The application of heterosis in rice production greatly improves rice yield, but hybrid vigor cannot be preserved in the offspring of hybrids due to...
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Optical Tweezers to Investigate the Structure and Energetics of Single-Stranded DNA-Binding Protein-DNA Complexes
Optical tweezers enable the isolation and mechanical manipulation of individual nucleoprotein complexes. Here, we describe how to use this technique...
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Double Crossover Approach to Inactivate Target Gene in Cyanobacteria
Anabaena sp. PCC7120 (hereafter Anabaena 7120) is a nitrogen-fixing, filamentous cyanobacterium. Given its diverse metabolism, it serves as an...
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Tethered Function Assays to Elucidate the Role of RNA-Binding Proteins
The fate of each RNA molecule is strongly determined by RNA-binding proteins (RBPs) which accompany transcripts from its synthesis to its...