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Enzymatic Release of Glycoprotein N-Glycans and Fluorescent Labeling
Glycosylation, one of the most frequent protein posttranslational modifications, is involved in the mechanisms of cell-cell interactions and immune...
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Detection of Posttranslational Modifications by Fluorescent Staining of Two-Dimensional Gels
Posttranslational modifications (PTMs) are key to the regulation of functional activities of proteins. Quantitative and qualitative information about...
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S-Acylation of Proteins
Palmitoylation or S-acylation is the posttranslational attachment of fatty acids to cysteine residues and is common among integral and peripheral...
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Characterization of Site-Specific N-Glycosylation
Even if a consensus sequence has been identified for a posttranslational modification, the presence of such a sequence motif only indicates the...
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Functional Analysis of HDACs in Tumorigenesis
HDACs, originally described as histone modifiers, have recently been demonstrated to modify a variety of other proteins that are involved in diverse...
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Experimental Approaches to Investigate the Role of Helicase Acetylation in Regulating R-Loop Stability
R-loops are three-stranded nucleic acid structures composed of a DNA-RNA heteroduplex and a displaced single-stranded DNA. Although R-loops serve...
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High-Resolution Mass Spectrometry to Identify and Quantify Acetylation Protein Targets
The dynamic nature of protein posttranslational modification (PTM) allows cells to rapidly respond to changes in their environment, such as...
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Isolation of Proteins on Nascent Chromatin and Characterization by Quantitative Mass Spectrometry
Replication-coupled chromatin assembly is a very dynamic process that involves not only the replication fork machinery but also chromatin-related...
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Using Yeast as a Model Organism to Study the Functional Roles of Histone Acetylation in DNA Excision Repair
Histone acetylation plays important roles in regulating DNA metabolic processes, including many DNA repair pathways. The nucleotide excision repair...
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Lysine Acetylation of Proteins and Its Characterization in Human Systems
Posttranslational acetylation modifications of proteins have important consequences for cell biology, including effects on protein trafficking and...
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α-Amidated Peptides: Approaches for Analysis
α-Amidation is a terminal modification in peptide biosynthesis that can itself be rate limiting in the overall production of bioactive α-amidated...
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An Approach for the Identification of Proteins Modified with ISG15
Interferon-stimulated gene 15 (ISG15) encodes a protein that is most upregulated by type I interferon stimulation and, upon activation, is conjugated...
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Site-Specific Lysine Acetylation Stoichiometry Across Subcellular Compartments
Posttranslational modifications of proteins control many complex biological processes, including genome expression, chromatin dynamics, metabolism,...
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Chromatographic Profiling of N-Glycans
Most glycoproteins carry a very heterogeneous mixture of oligosaccharides and even a single glycosylation site of a pure glycoprotein is often...
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Analysis of Methylation, Acetylation, and Other Modifications in Bacterial Ribosomal Proteins
A wide variety of posttranslational modifications of expressed proteins are known to occur in living organisms (Krishna R, Wold F. Post-translational...
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Biochemical and Cellular Assays to Assess the Effects of Acetylation on Base Excision Repair Enzymes
Protein posttranslational modifications (PTMs), including acetylation, have emerged as important regulators for controlling many cellular processes....
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Assessment of SIRT2 Inhibitors in Mouse Models of Cancer
New therapeutics directed against established and novel molecular targets are urgently needed to intervene against cancer. Recently, it was reported...
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Glycosylation of Type I Collagen
Fibrillar type I collagen is the most abundant structural protein in most tissues and organs. One of the unique and functionally important...
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Molecular and Cellular Characterization of SIRT1 Allosteric Activators
SIRT1 is an NAD+-dependent lysine deacetylase that promotes healthy aging and longevity in diverse organisms. Small molecule allosteric activators of...
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Quantification of In Vitro Protein Lysine Acetylation by Reversed Phase HPLC
Protein lysine acetylation is a reversible posttranslational modification that is catalyzed by a group of enzymes that are collectively referred to...