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Stem Cells in CNS and Cardiac Regeneration
The central nervous system (CNS) and the heart muscle regenerate poorly after injury, yet evidence is mounting that both harbor cells capable of...
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Stable Expression by Lentiviral Transduction of Cells
Lentiviral gene transfer represents a versatile and powerful method for genetic transduction of many cell lines and primary cells including...
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Recombinant Protein Expression in Mammalian Cells Methods and Protocols
This fully updated volume explores notable developments in the field of mammalian cell-based recombinant protein production. Beginning with methods...
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Analyzing Posttranslational Modifications in Single Cells
With the rapid expansion of capabilities in the analysis of proteins in single cells, we can now identify multiple classes of protein...
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Minimal Cells and Genome Minimization: Top-Down and Bottom-Up Approaches to Construct Synthetic Cells
Bioproduction of high-value molecules has traditionally been carried out using engineered host cells. However, toxic molecules and membrane proteins...
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Genetic Code Expansion in Mammalian Cells
The expansion of the genetic code has enabled the incorporation of noncanonical amino acids (ncAAs) into a defined site of proteins. By introducing...
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Metal Phosphates/Phosphonates for Fuel Cells
The rapid development of the era of social electrification has put forward higher requirements for sustainable and clean energy. The development of...
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Galvanic Cells
Let's have a closer look to batteries, accumulators an the phenomena of electrochemical corrosion.
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Low-Temperature Ammonia Fuel Cells
Low-temperature fuel cells are one of the promising energy conversion devices that use ammonia as a fuel. In this chapter, we focus on fuel cells...
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PEI-Mediated Transient Gene Expression in CHO Cells
We describe a method for polyethyleneimine (PEI)-mediated transient transfection of suspension-adapted Chinese hamster ovary (CHO-DG44) cells for...
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Coiled-Coil Interaction Toolbox for Engineering Mammalian Cells
Protein interactions play a crucial role in a variety of biological processes. Therefore, regulation of these interactions has received considerable...
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Microchip for Immunomagnetic Sorting of Circulating Tumor Cells (CTCs)
Circulating tumor cells (CTCs) isolated directly from whole blood opens new perspectives for cancer monitoring and the development of personalized...
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Imaging Analysis of Neurotrophic Effects by CCN2 Protein in Neuronal Precursor Cells Derived from Human-Induced Pluripotent Stem Cells
Human-induced pluripotent stem cells (hiPSCs) are useful tools to examine human neuronal maturation processes. In this chapter, we describe the...
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Realizing Antithetic Integral Feedback Control in Mammalian Cells
Genetic circuit engineering has emerged as a powerful methodology to program the behavior of mammalian cells to respond to internal and external...
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Flow Cytometry Quantification of Transient Transfections in Mammalian Cells
Flow cytometry is a powerful quantitative assay supporting high-throughput collection of single-cell data with a high dynamic range. For flow...
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In Vitro Generation of Megakaryocytes from Engineered Mouse Embryonic Stem Cells
The in vitro differentiation of pluripotent stem cells into desired lineages enables mechanistic studies of cell transitions into more mature states...
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A versatile engineered extracellular vesicle platform simultaneously targeting and eliminating senescent stromal cells and tumor cells to promote tumor regression
Chemotherapy is an important therapeutic approach for malignant tumors for it triggers apoptosis of cancer cells. However, chemotherapy also induces...
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Fructobacillus fructosus OS-1010 strain stimulates intestinal cells to secrete exosomes that activate muscle cells
Fructobacillus is a lactic-acid bacterium recently identified in fructose-rich environments. Fructobacillus is also known to exhibit unusual growth...
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Carbon Nanotubes in Carbon Electrode Based Perovskite Solar Cells
Perovskite photovoltaics have emerged as a cost-effective, fast develo** technology with interesting light harvesting properties allowing for...
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Selective elimination of tumorigenic cells from mixed culture of normal and tumorigenic cells using hybrid liposomes aimed at realizing of cell therapy
While induced pluripotent stem (iPS) cells are expected to be a cell source for regenerative medicine, they also have tumorigenic properties owing to...
