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Absolute Quantitative Targeted Proteomics Assays for Plasma Proteins
Preclinical and clinical trials require rapid, precise, and multiplexed analytical methods to characterize the complex samples and to allow...
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Depletion of abundant plasma proteins for extracellular vesicle proteome characterization: benefits and pitfalls
Blood extracellular vesicles (EVs) play essential roles in cell–cell communication and their molecular cargo is a promising source of disease...
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Atmospheric Cold Plasma-Induced Changes in Milk Proteins
AbstractThe growing interest in minimal processing of foods due to its mild effects on product quality and taste has led to an increase in the...
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Offline Peptide Fractionation and Parallel Reaction Monitoring MS for the Quantitation of Low-Abundance Plasma Proteins
Mass spectrometry (MS)-based protein quantitation is an attractive means for research and diagnostics due to its high specificity, precision,...
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High-Throughput Proteome Profiling of Plasma and Native Plasma Complexes Using Native Chromatography
We describe a high-throughput method for co-fractionation mass spectrometry (CF-MS) profiling for native plasma protein profiling. CF-MS allows the...
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DIGE Analysis of Immunodepleted Plasma
This chapter focuses on upstream immunodepletion of high-abundance proteins from plasma samples and subsequent analysis by fluorescence...
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Investigation of native and aggregated therapeutic proteins in human plasma with asymmetrical flow field-flow fractionation and mass spectrometry
Physiochemical degradation of therapeutic proteins in vivo during plasma circulation after administration can have a detrimental effect on their...
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Engineering Fluorescent Proteins
Green fluorescent protein from the jellyfish Aequorea victora (GFP) and GFP-like proteins from Anthozoa species encode light-absorbing chromophores...
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Preparation of Cryoprecipitate and Cryo-depleted Plasma for Proteomic Research Analysis
When frozen plasma is slowly thawed in cold conditions (1–6 °C), high-molecular-weight plasma proteins precipitate forming a concentrate known as...
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An Optimized Data-Independent Acquisition Strategy for Comprehensive Analysis of Human Plasma Proteome
Cartography of the plasma proteome remains technically challenging, primarily due to the abundance and dynamic range of plasma proteins and their...
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Bioinformatics Tools and Knowledgebases to Assist Generating Targeted Assays for Plasma Proteomics
In targeted proteomics experiments, selecting the appropriate proteotypic peptides as surrogate for the target protein is a crucial pre-acquisition...
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Rapid and Quantitative Enrichment of Peptides from Plasma for Mass Spectrometric Analysis
Mass spectrometric analysis of peptides enables the assignment of their exact mass and confirmation of all or a significant portion of the peptide’s...
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High-Throughput Plasma Proteomic Profiling
Plasma and serum are rich sources of proteins that are commonly used for clinical proteome profiling and biomarkers discovery. However,...
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The development of a novel zeolite-based assay for efficient and deep plasma proteomic profiling
Plasma proteins are considered the most informative source of biomarkers for disease diagnosis and monitoring. Mass spectrometry (MS)-based...
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Protocol for Plasma Extracellular Vesicle and Particle Isolation and Mass Spectrometry-Based Proteomic Identification
Plasma extracellular vesicles and particles (EVPs) are enriched in biomolecules that reflect individuals’ physiological and pathological states....
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Dietary Proteins and Health
Proteins are a significant component of the food we eat and are involved in all the diverse processes of the body. They are a type of biomolecule...
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Collection of Plasma Samples in Areas with Limited Healthcare Access
Regular monitoring of various biomarkers and molecular panels in plasma can significantly help to prevent disease onset and improve its management...
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Proteins from Biomass Residue
The increasing demand for alternative proteins, driven by population growth, urbanization, and the growing popularity of vegetarian and vegan...
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Fluorescence Correlation Spectroscopy with Autofluorescent Proteins
Fluorescence correlation spectroscopy (FCS) is a versatile technique operating at the single-molecule level, that successfully meets many challenges...
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Strategies to Enrich, Identify, and Characterize Glycoproteome in Blood Plasma Using Liquid Chromatography High-Resolution Mass Spectrometry
Blood and blood-derived components such as plasma and serum are considered as excellent resources that can be utilized to understand the biology of...