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Collection of Plasma Samples in Areas with Limited Healthcare Access

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Serum/Plasma Proteomics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2628))

Abstract

Regular monitoring of various biomarkers and molecular panels in plasma can significantly help to prevent disease onset and improve its management and final outcomes. Many groups can benefit from monitoring programs focusing on the prevention of cardiovascular diseases, evaluation of environmental exposure impacts, or the prevention/management of cancer. Improvement in therapeutic options in part due to targeted therapeutic agents and monoclonal antibody therapies has led to a significant sized population that can be described as “cancer survivors.” These patients, although in remission from their original disease, are at significant risk for the recurring disease and must be monitored for adverse events. Monitoring is, however, not an easy task; requiring a high level of complexity in lab facilities and blood/plasma sampling, collection, and storage must occur under tightly controlled conditions. These demanding circumstances are especially difficult to attain in rural areas and in historically marginalized populations. The Telimmune Plasma Separation Card (TPS card or TPSC) has been developed to enable diagnostic plasma sampling, collection, and stabilization in locations that may be remote to laboratory or clinic. The TPSC requires a drop of blood applied to a top of a separation system consisting of a separation membrane and collection disk. In 3 min, the TPSC device separates plasma from erythrocytes and deposits a defined volume of plasma into a collection disc which is air-dried for 15 min to deliver a stabilized, volumetric plasma sample, which may be stored or shipped at ambient temperatures with minimal biological risk. Extraction of proteins and metabolites is then achieved in well-equipped laboratories using protocols discussed in this chapter.

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Acknowledgments

This work was supported by grants from the National Institute of Health (P20 RR017675 and P20 GM104320).

Conflicts of Interest

Jiri Adamec is co-founder of Novilytic and Telimmune. Camila Pereira Braga, Alicia Johnson, and Pedro de Magalhães Padilha have nothing to disclose.

Author information

Authors and Affiliations

  1. Department of Biochemistry, University of Nebraska-Lincoln, Lincoln, NE, USA

    Alicia Johnson, Camila Braga & Jiri Adamec

  2. Redox Biology Center, University of Nebraska-Lincoln, Lincoln, NE, USA

    Alicia Johnson, Camila Braga & Jiri Adamec

  3. Institute of Biosciences, São Paulo State University (UNESP), Botucatu, São Paulo, Brazil

    Pedro de Magalhães Padilha

Authors
  1. Alicia Johnson
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  2. Camila Braga
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  3. Pedro de Magalhães Padilha
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  4. Jiri Adamec
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Corresponding author

Correspondence to Jiri Adamec .

Editor information

Editors and Affiliations

  1. Molecular Proteomics, Baker Heart and Diabetes Institute, Melbourne, VIC, Australia

    David W. Greening

  2. Department of Biochemistry and Chemistry, School of Agriculture, Biomedicine and Environment, La Trobe Institute for Molecular Science, Melbourne, VIC, Australia

    Richard J. Simpson

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© 2023 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Johnson, A., Braga, C., de Magalhães Padilha, P., Adamec, J. (2023). Collection of Plasma Samples in Areas with Limited Healthcare Access. In: Greening, D.W., Simpson, R.J. (eds) Serum/Plasma Proteomics. Methods in Molecular Biology, vol 2628. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2978-9_2

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  • DOI: https://doi.org/10.1007/978-1-0716-2978-9_2

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2977-2

  • Online ISBN: 978-1-0716-2978-9

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