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Detection of dopamine levels using a polydiacetylene liposomal aequorin bioluminescent device with octadecylboronic acid
The development of an easy-to-use and rapid method for the determination of dopamine levels is desirable for the diagnosis of neurological...
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Soft Crystal Chemiluminescence Systems Using Organic Peroxides
Chemiluminescence (CL) is a phenomenon in which a chemical reaction produces an excited-state product that emits light. Taking advantage of this...
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Longer characteristic wavelength in a novel engineered photoprotein Mnemiopsin 2
We designed two mutants of photoprotein Mnemiopsin 2 (Mn2) including M52I and V144I, where the mutations were applied in the EF-hand loops I and III....
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Cell-Based Biosensor for Rapid Screening of Pathogens and Toxins
Exposure to pathogens, toxins, and pollutants is a major public health concern. Often air, water, and food are the major sources. Efforts to develop...
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Unusual shift in the visible absorption spectrum of an active ctenophore photoprotein elucidated by time-dependent density functional theory
Active hydromedusan and ctenophore Ca 2+ -regulated photoproteins form complexes consisting of apoprotein and strongly non-covalently bound...
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Marine Bioluminescence with Dehydrocoelenterazine, an Imidazopyrazinone Compound
Heterocycles play important roles in bioluminescence. Among them, the imidazopyrazinone compounds are utilized for light emission in some marine...
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Coelenterazine-Dependent Luciferases: Properties and Application in Molecular Analysis
AbstractThis review presents the data on the structure of bioluminescent systems of marine animals that use coelenterazine-dependent luciferases as...
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Applications of CPPs in Genome Editing of Plants
Cell penetrating peptides (CPPs) are short peptides that are able to translocate themselves and their cargo into cells. The progressive and...
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The interaction of C-terminal Tyr208 and Tyr13 of the first α-helix ensures a closed conformation of ctenophore photoprotein berovin
Light-sensitive Ca 2+ -regulated photoprotein berovin is responsible for the bioluminescence of the cteno-phore Beroe abyssicola. It shares many...
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Microbial Biosensors for the Detection of Organic Pollutants
Over the last 30 years, numerous scientific publications have described the design, construction, testing, and characterization of diverse whole-cell...
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Immobilization of Nucleic Acids Using Biotin-Strept(avidin) Systems
There are several advantages for using biotin-streptavidin/avidin (strept(avi-din)) systems to immobilize nucleic acids and other molecules. These...
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CdTe quantum dots with green fluorescence generated by bioluminescence resonance energy transfer from aequorin
The need for external excitation sources limits the utility of quantum dots (QDs) in multiplexed detection schemes and in in vivo imaging, because it...
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Cell-Based Biosensor for Rapid Screening of Pathogens and Toxins
Exposure to pathogens, toxins, and pollutants is a major public health concern. Often air, water, and food are the major sources. Efforts to develop...
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Bacterial bioluminescence assay for bioanalysis and bioimaging
Bioluminescence occurs through a chemical reaction in organisms that spontaneously produce light. Luminescent bacteria are unique among...
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Glowing wonders: exploring the diversity and ecological significance of bioluminescent organisms in Brazil
AbstractBioluminescence, the emission of light by living organisms, is a captivating and widespread phenomenon with diverse ecological functions....
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Endpoint and Kinetic Approaches for Assessing Transfection Efficacy in Mammalian Cell Culture
The efficacy of transfection reagents and nanoparticles is often assessed by measuring levels of expressed reporter protein. Fluorescence and...
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Detection of Biological Warfare Agents Using Biosensors
This chapter surveys the current detection technologies used in commercially available biosensors for identifying biological warfare agents (BAs)....
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Tuning the fluorescence of calcium-discharged photoprotein obelin via mutating at the His22-Phe88-Trp92 triad – a QM/MM study†
The fluorescence (FL) of calcium-discharged photoprotein (CaDP) can be altered by easily mutating CaDP without modifying coelenteramide (CLM), which...
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Aquaculture of “Non-Food Organisms” for Natural Substance Production
Marine invertebrates are already sources of commercially important secondary metabolites and may become even more so as knowledge on marine...