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Affinity-based isolation of extracellular vesicles and the effects on downstream molecular analysis
Extracellular vesicles (EVs) are transport vesicles with diameters ranging from 30 to 1000 nm, secreted by cells in both physiological and...
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Large-Scale Purification of Natural Ice-Binding Proteins by the Falling Water Ice Purification Method
We describe here a generic technique for purification of ice-binding proteins (IBPs) using a commercial ice machine. The method, which is called...
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Purification of Therapeutic Antibodies by Protein A Affinity Chromatography
Protein A affinity chromatography is widely used for capturing therapeutic antibodies. It offers high binding capacity, selectivity, and resin...
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Regulation of Gene Expression by Synthetic DNA-Binding Ligands
During the past 20 years, polyamides have evolved from the natural product distamycin to a new class of programmable heterocyclic oligomers that bind...
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Antiparasitic Activity and Potential Active Compounds from Azadirachta Indica Revealed by Affinity Ultrafiltration Chromatography–Mass Spectrometry with Acetylcholinesterase and Lactate Dehydrogenases
Azadirachta indica ( A. indica ) has traditionally been used to treat parasitic diseases, while its antiparasitic compounds and their mechanism remain...
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Purification and Biochemical Characterization of the DNA Binding Domain of the Nitrogenase Transcriptional Activator NifA from Gluconacetobacter diazotrophicus
NifA is a σ 54 activator that turns on bacterial nitrogen fixation under reducing conditions and when fixed cellular nitrogen levels are low. The...
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Antibody Purification Using Affinity Chromatography
Antibodies are an integral part of many biological assays and biotherapeutics. However, the sources from which antibodies are derived frequently...
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Positive and Negative Affinity Chromatography to Purify Norovirus P-Domain Protein
Affinity chromatography enables the separation and isolation of proteins of interest from complex milieu of biochemicals. Nickel-charged affinity...
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Cyanine Dye–DNA Interactions: Intercalation, Groove Binding, and Aggregation
The cyanine dyes are among the oldest classes of synthetic compounds but continue to find applications in a variety of fields. In many cases, the...
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In silico approach to probe the binding affinity between OMVs harboring the ZEGFR affibody and the EGF receptor
There is a growing interest in designing a nanocarrier containing an EGFR targeting affibody to direct toward cancer cells. Here, cytolysin A was...
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Study on the S239D/I332E mutation effect on the kinetics of Fc–Fc receptor binding through transition complex theory
The S239D/I332E mutation in both chain A and chain B of Fc is widely employed to enhance the binding affinity of Fc to its receptor (FcγRIIIa)....
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Strategies for the enhancement of anti-cancer effect of phosphodiesterase type 5 inhibitors using drug binding fusion proteins
Drug repurposing is a rapidly growing for the development of new therapeutic agents. Among them, phosphodiesterase-5 (PDE5) inhibitors, which show...
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Radiolabeling fingolimod with technetium-99 m and evaluating its biological affinity by in vitro method
Fingolimod (FTY-720) is the first oral medication approved by the food and drug administration (FDA) for the treatment of multiple sclerosis. It acts...
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Affinity Peptide-based Electrochemical Biosensor for the Highly Sensitive Detection of Bovine Rotavirus
Bovine diarrhea is a major concern in the global bovine industry because it can cause significant financial damage. Of the many potential infectious...
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Affinity-Guided Isolation and Identification of Procyanidin B2 from Mangosteen (Garcinia mangostana L.) Rinds and its In Vitro LPS Binding and Neutralization Activities
Garcinia mangostana L. (mangosteen) is a tropical fruit that has been used for medicinal purposes in Southeast Asia for centuries. With an interest...
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Biodegradation of anionic polyacrylamide by manganese peroxidase: docking, virtual mutation based on affinity, QM/MM calculation and molecular dynamics simulation
Manganese peroxidase (Mn P) is capable of effectively degrading anionic polyacrylamide (HPAM). However, the interaction of Mn P with HPAM at...
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Multiscale Molecular Dynamics Simulations of Ice-Binding Proteins
Ice-binding proteins (IBPs) are a diverse class of proteins that are essential for the survival of organisms in cold conditions. IBPs are diverse in...
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Evaluating the chaos game representation of proteins for applications in machine learning models: prediction of antibody affinity and specificity as a case study
ContextMachine learning techniques are becoming increasingly important in the selection and optimization of therapeutic molecules, as well as for the...
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How the concept of chemical affinity evolved and how it contributed to chemical science
This historiographic paper traces the evolution of the concept of chemical affinity from its antique origins until the end of the twentieth century....
