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Development of KoRV-pseudotyped lentiviral vectors for efficient gene transfer into freshly isolated immune cells
Allogeneic cell therapies, such as those involving macrophages or Natural Killer (NK) cells, are of increasing interest for cancer immunotherapy....
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New biflavonoids isolated from Xylia kerrii leaves extract with selective cytotoxicity against MH7A human rheumatoid arthritis synovial fibroblast cells
Three new biflavonoids ( 1 – 3 ) and two known flavonoids ( 4 , 5 ) were isolated from Xylia kerrii collected in Thailand. Compounds 1 – 5 showed selective...
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Cell fusing agent virus isolated from Aag2 cells does not vertically transmit in Aedes aegypti via artificial infection
BackgroundCell fusing agent virus (CFAV) was the first insect-specific virus to be characterized, and has been reported to negatively influence the...
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Production of Stem Cell Antigen 1 Sca-1/Ly-6A/E by Freshly Isolated NK Cells Cultured with Relevant Cytokines
Activated NK cells in appropriate conditions are known to express stem cell antigen 1 (Sca-1/Ly-6A/E). To investigate its production, NK cells...
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Lobetyolin, a Q-marker isolated from Radix Platycodi, exerts protective effects on cisplatin-induced cytotoxicity in HEK293 cells
This study investigated the protective effect of lobetyolin (LBT), a Q-marker isolated from the roots of Platycodon grandiflorum (Radix Platycodi),...
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Amine-modified nanoplastics promote the procoagulant activation of isolated human red blood cells and thrombus formation in rats
BackgroundMicroplastics (MPs) and nanoplastics (NPs) formed from decomposed plastic are increasing environmental threats. Although MPs and NPs...
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Basket Cells
Basket cells are GABAergic interneurons located in the basal one-third of the molecular layer of the cerebellum. Basket cells receive excitatory...
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Isolation of Live Immune Cells from the Tumor Microenvironment by FACS
Isolation of live cells from the tumor microenvironment (TME) has represented a challenge, particularly from metastatic nodules that need to be...
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Methoxyflavones isolated from the whole plant of Scutellaria rubropunctata Hayata var. rubropunctata promote osteoblast differentiation in MC3T3-E1 cells
In this study, we isolated two new methoxyflavones ( 1 and 2 ) and eight known methoxyflavones ( 3 – 10 ) from the whole plant of Scutellaria rubropunctata ...
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Cancer Stem Cells
Cancer stem cells (CSCs) share traits with healthy stem or precursor cells, which include having the capacity to self-renew and differentiate into...
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Overview: Cancer Stem Cells
Cancer has become a global issue and retains a central place in fatality rates among the wide variety of diseases known the world over, and the...
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Isolation and Culture of Human Umbilical Vein Endothelial Cells (HUVECs)
This protocol describes a simple and an economical method for isolation of endothelial cells from human umbilical vein. Umbilical cord is easily...
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Development of Immune Cell Therapy Using T Cells Generated from Pluripotent Stem Cells
In the field of cancer immunotherapy, the effectiveness of a method in which patient-derived T cells are genetically modified ex vivo and...
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In Vitro Tumorigenic Assay: A Tumor Sphere Assay for Cancer Stem Cells
Cancer stem cells (CSCs) represent a subpopulation of tumor cells that are thought to be responsible for therapy resistance, recurrence, and...
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Effect of isolated grandivittin from Ferulago trifida Boiss. (Apiaceae) on the proliferation and apoptosis of human lung cancer A549 cells
Lung cancer is one of the deadliest cancers in the world. Introducing new promising agents can help the chemotherapeutic management of cancer. In the...
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Gene Editing in Hematopoietic Stem Cells
Hematopoietic stem cells (HSCs) can be isolated and collected from the body, genetically modified, and expanded ex vivo. The invention of innovative...
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Autophagy in Embryonic Stem Cells and Neural Stem Cells
AutophagyAutophagy is a conserved cytoprotective catabolic pathway that plays a crucial role in cellular turnover and homeostasis in eukaryotic...
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Cancer Stem Cells: Current Challenges and Future Perspectives
Despite major advances in health care including improved diagnostic tools, robust chemotherapeutic regimens, advent of precision, adjuvant and...
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Evaluation of the antiproliferative effect of β-sitosterol isolated from Combretum platypetalum Welw. ex M.A. Lawson (Combretaceae) on Jurkat-T cells and protection by glutathione
Combretum species are distributed widely in Southern Africa and are known for their medicinal properties. The species have potential as sources of...
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Detection of Nanoparticle-Mediated Total Oxidative Stress in T Cells Using CM-H2DCFDA Dye
Oxidative stress is commonly observed in cells following exposure to nanoparticles. Both negative (e.g., cytotoxicity and inflammation) and...