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Functional characteristics of Th1, Th17, and ex-Th17 cells in EAE revealed by intravital two-photon microscopy
BackgroundT helper (Th) 17 cells are a highly plastic subset of T cells, which in the context of neuroinflammation, are able to acquire pathogenic...
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Vascular Hyperpermeability Methods and Protocols
This volume focuses on the latest research methods and techniques used by researchers to study vascular permeability/hyperpermeability in a science...
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Label-Free Multimodal Multiphoton Intravital Imaging
Label-free intravital optical imaging is an emergent visualization tool that is not only useful for basic biological research, but also for...
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Label-Free Monitoring of Endometrial Cancer Progression Using Multiphoton Microscopy
Endometrial cancer is the most common gynecological cancer in the developed world. However, the accuracy of current diagnostic methods is still...
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Imaging of Extravasation of Splenocytes in the Dorsal Skinfold Window Chamber
Infiltration of immune cells into the tumor is one of the major drivers of antitumor immune response, which can direct the outcome of anticancer...
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Complete Visualization of T Follicular Helper Cells in Germinal Centers by Light Sheet Fluorescence Microscopy
Long-lasting immunity depends on generation of antibody forming cells in germinal centers (GCs). Conventional methods such as immunohistology and...
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Microvascular Endothelial Glycocalyx Surface Layer Visualization and Quantification
As a primary interface between the blood and underlying vascular wall, the endothelial glycocalyx layer is common to all blood vessels and covers the...
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Intravital three-dimensional bioprinting
Fabrication of three-dimensional (3D) structures and functional tissues directly in live animals would enable minimally invasive surgical techniques...
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Intravital imaging of orthotopic and ectopic bone
Bone homeostasis is dynamically regulated by a balance between bone resorption by osteoclasts and bone formation by osteoblasts. Visualizing and...
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Long-term self-renewing stem cells in the adult mouse hippocampus identified by intravital imaging
Neural stem cells (NSCs) generate neurons throughout life in the mammalian hippocampus. However, the potential for long-term self-renewal of...
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Analysis of the Effect of IL-1β on Blood—Brain Barrier Permeability in M6 Glioma Mouse Model Using Intravital Microscopy
We studied the effect of IL-1β on the permeability of brain capillaries in healthy mice. Intravital microscopy demonstrated that parenteral...
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Measurement of Endothelial Barrier Function in Mouse Models of Cerebral Cavernous Malformations Using Intravital Microscopy
Vascular permeability is a major function of the microvasculature that is regulated by multiple factors including blood pressure, blood viscosity,...
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Subcellular spatio-temporal intravital kinetics of aflatoxin B1 and ochratoxin A in liver and kidney
Local accumulation of xenobiotics in human and animal tissues may cause adverse effects. Large differences in their concentrations may exist between...
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Imaging of Neutrophils and Neutrophil Extracellular Traps (NETs) with Intravital (In Vivo) Microscopy
As we have learned during recent years, neutrophils are not just simple foot soldiers of the innate immune system with a restricted set of...
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JAK inhibition ameliorates bone destruction by simultaneously targeting mature osteoclasts and their precursors
BackgroundRheumatoid arthritis (RA) is characterized by chronic inflammation and resultant cartilage/bone destruction because of aberrantly activated...
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Dynamic encounters with red blood cells trigger splenic marginal zone B cell retention and function
Spleen marginal zone (MZ) B cells are important for antibody responses against blood-borne antigens. The signals they use to detect exposure to blood...
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Single nephron glomerular filtration rate measured by linescan multiphoton microscopy compared to conventional micropuncture
Renal micropuncture, which requires the direct access to the renal tubules, has for long time been the technique of choice to measure the single...
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Arthritis-associated osteoclastogenic macrophage, AtoM, as a key player in pathological bone erosion
Osteoclasts are myeloid lineage cells with a unique bone-destroying ability that maintains bone homeostasis together with bone formation by...
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Multiscale optical and optoacoustic imaging of amyloid-β deposits in mice
Deposits of amyloid-β (Aβ) in the brains of rodents can be analysed by invasive intravital microscopy on a submillimetre scale, or via whole-brain...
