-
Article
Open AccessEnhancing the biosynthesis of taxadien-5α-yl-acetate in Escherichia coli by combinatorial metabolic engineering approaches
Biosynthesis of paclitaxel (Taxol™) is a hot topic with extensive and durable interests for decades. However, it is severely hindered due to the very low titers of intermediates. In this study, Escherichia coli w...
-
Article
Open AccessHydroxylases involved in terpenoid biosynthesis: a review
Terpenoids are pervasive in nature and display an immense structural diversity. As the largest category of plant secondary metabolites, terpenoids have important socioeconomic value in the fields of pharmaceut...
-
Article
Open AccessEngineering a norcoclaurine synthase for one-step synthesis of (S)-1-aryl-tetrahydroisoquinolines
Tetrahydroisoquinoline alkaloids (THIQAs) are ubiquitous compounds with important pharmaceutical and biological activity. Their key N-heterocyclic structural motifs are synthesised via Pictet–Spengler (P–S) react...
-
Article
Open AccessRegiospecific C–H amination of (−)-limonene into (−)-perillamine by multi-enzymatic cascade reactions
(−)-Limonene, one of cyclic monoterpenes, is an important renewable compound used widely as a key building block for the synthesis of new biologically active molecules and fine chemicals. (−)-Perillamine, as d...
-
Article
Open AccessConstruction of an Escherichia coli cell factory to synthesize taxadien-5α-ol, the key precursor of anti-cancer drug paclitaxel
Paclitaxel (Taxol™), an alkaloid of diterpenoid family, is one of the most widely used anti-cancer drugs due to its effectiveness against a variety of tumors. Rather than directly extraction and chemical synth...
-
Article
Open AccessMining methods and typical structural mechanisms of terpene cyclases
Terpenoids, formed by cyclization and/or permutation of isoprenes, are the most diverse and abundant class of natural products with a broad range of significant functions. One family of the critical enzymes in...
-
Article
Influence of PTGS1, PTGFR, and MRP4 genetic variants on intraocular pressure response to latanoprost in Chinese primary open-angle glaucoma patients
The purpose of this study is to evaluate the association between variants in prostaglandin-endoperoxide synthase 1 (PTGS1), prostaglandin F (2α) receptor (PTGFR), and multidrug resistance protein 4 (MRP4) genes a...
-
Article
Synthesis and characterization of HALS/UV-absorbers bifunctionalized core-shell elastomer and Its application in polyoxymethylene
Poly(BA-MMA-PMPA-BPMA), a core-shell polyacrylate elastomer containing hindered amine light stabilizer (HALS) and benzophenone UV-absorber (UVA) in the shell phase, was prepared by two-stage seeded emulsion po...
