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Protocol
Cloning Differentially Expressed Genes Using Rapid Subtraction Hybridization (RaSH)
Differential gene expression represents the entry point for comprehending complex biological processes. In this context, identification and cloning of differentially expressed genes represent critical elements...
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Article
Potential molecular mechanism for rodent tumorigenesis: mutational generation of Progression Elevated Gene-3 (PEG-3)
Progression Elevated Gene-3 (PEG-3) was cloned using subtraction hybridization as an upregulated transcript associated with transformation and tumor progression of rat embryo fibroblast cells. PEG-3 is a unique g...
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Article
β-Lactam antibiotics offer neuroprotection by increasing glutamate transporter expression
The β-lactam antibiotics — penicillin and its semisynthetic derivatives — act by inhibiting bacterial synthetic pathways, and their success has in part been because they have little effect on their hosts. So i...
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Article
Induction of reactive oxygen species renders mutant and wild-type K-ras pancreatic carcinoma cells susceptible to Ad.mda-7-induced apoptosis
Pancreatic cancer is exceptionally aggressive with no long-term effective therapy. Current interventional approaches, including surgery, radiation and/or chemotherapy, have done little to quell the mortality a...
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Article
Effects of human immunodeficiency virus type 1 on astrocyte gene expression and function: Potential role in neuropathogenesis
Neurodegeneration and dementia caused by human immunodeficiency virus type 1 (HIV-1) infection of the brain are common complications of acquired immunodeficiency syndrome (AIDS). Introduction of highly active ...
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Article
Bcl-2 and Bcl-xL differentially protect human prostate cancer cells from induction of apoptosis by melanoma differentiation associated gene-7, mda-7/IL-24
Subtraction hybridization identified melanoma differentiation associated gene-7, mda-7, in the context of terminally differentiated human melanoma cells. Based on its structure, cytokine-like properties and propo...
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Article
Identification of gene products suppressed by human immunodeficiency virus type 1 infection or gp120 exposure of primary human astrocytes by rapid subtraction hybridization
Neurodegeneration and human immunodeficiency virus type 1 (HIV-1)-associated dementia (HAD) are the major disease manifestations of HIV-1 colonization of the central nervous system (CNS). In the brain, HIV-1 r...
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Article
Melanoma differentiation associated gene-7, mda-7/IL-24, selectively induces growth suppression, apoptosis and radiosensitization in malignant gliomas in a p53-independent manner
Malignant gliomas are extremely aggressive cancers currently lacking effective treatment modalities. Gene therapy represents a promising approach for this disease. A requisite component for improving gene-base...
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Article
Identification and cloning of human astrocyte genes displaying elevated expression after infection with HIV-1 or exposure to HIV-1 envelope glycoprotein by rapid subtraction hybridization, RaSH
Neurodegeneration and dementia are common complications of AIDS caused by human immunodeficiency virus type 1 (HIV-1) infection of the central nervous system. HIV-1 target cells in the brain include microglia,...
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Chapter
Mda-7, A Novel Melanoma Differentiation Associated Gene with Promise for Cancer Gene Therapy
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Article
Genomic structure, chromosomal localization and expression profile of a novel melanoma differentiation associated (mda-7) gene with cancer specific growth suppressing and apoptosis inducing properties
Abnormalities in cellular differentiation are frequent occurrences in human cancers. Treatment of human melanoma cells with recombinant fibroblast interferon (IFN-β) and the protein kinase C activator mezerein...
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Article
Cooperation between AP1 and PEA3 sites within the progression elevated gene-3 (PEG-3) promoter regulate basal and differential expression of PEG-3 during progression of the oncogenic phenotype in transformed rat embryo cells
Cancer is a progressive disease in which a tumor cell temporally develops qualitatively new transformation related phenotypes or a further elaboration of existing transformation associated properties. Subtract...
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Chapter and Conference Paper
Enhancement of Oncogene-Mediated Transformation in Cloned Rat Embryo Fibroblast (CREF) Cells by 3-Aminobenzamide
The carcinogenic process involves a complex series of genetic alterations in the evolving tumor cell resulting in changes in genes which induce both positive (oncogene) and negative (tumor suppressor) control ...
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Chapter
The Inhibitory Effect of Parvovirus H-1 on Cultured Human Cancer Cells or Transformed Cells
Hamster osteolytic virus H-l is an autonomous parvovirus. Its genome, consisting of a linear single-stranded DNA molecule of 5176 nucleotides, carries 3 structural genes of capsid and non-capsid protein; the v...
