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Article
Open AccessStandardizing HER2 immunohistochemistry assessment: calibration of color and intensity variation in whole slide imaging caused by staining and scanning
In the evaluation of human epidermal growth factor receptor 2 (HER2) immunohistochemistry (IHC) — one of the standard biomarkers for breast cancer— visual assessment is laborious and subjective. Image analysis...
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Article
Open AccessA pilot study of micro-CT-based whole tissue imaging (WTI) on endoscopic submucosal dissection (ESD) specimens
Endoscopic submucosal dissection can remove large superficial gastrointestinal lesions in en bloc. A detailed pathological evaluation of the resected specimen is required to assess the risk of recurrence after...
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Article
Open AccessAutomated 3D scoring of fluorescence in situ hybridization (FISH) using a confocal whole slide imaging scanner
Fluorescence in situ hybridization (FISH) is a technique to visualize specific DNA/RNA sequences within the cell nuclei and provide the presence, location and structural integrity of genes on chromosomes. A co...
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3-D Tissue Image Reconstruction from Digitized Serial Histologic Sections to Visualize Small Tumor Nests in Lung Adenocarcinomas
3-D histology has become an attractive technique providing insights into morphology of histologic specimens. However, existing techniques in generating 3-D views from a stack of whole slide images are sc...
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Article
Open AccessValidation of mitotic cell quantification via microscopy and multiple whole-slide scanners
The establishment of whole-slide imaging (WSI) as a medical diagnostic device allows that pathologists may evaluate mitotic activity with this new technology. Furthermore, the image digitalization provides an ...
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Article
Staining Correction in Digital Pathology by Utilizing a Dye Amount Table
The stained colors of the tissue components are popularly used as features for image analysis. However, variations in the staining condition of the histology slides prompt variations to the color distribution ...
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Article
Open Access3-dimensional digital reconstruction of the murine coronary system for the evaluation of chronic allograft vasculopathy
Chronic allograft vasculopathy (CAV) is a major mechanism of graft failure of transplanted organs in humans. Morphometric analysis of coronary arteries enables the quantitation of CAV in mouse models of heart ...
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Article
Open AccessConsistency and Standardization of Color in Medical Imaging: a Consensus Report
This article summarizes the consensus reached at the Summit on Color in Medical Imaging held at the Food and Drug Administration (FDA) on May 8–9, 2013, co-sponsored by the FDA and ICC (International Color Con...
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Article
Micro-computed tomography (Micro-CT): a novel approach for intraoperative breast cancer specimen imaging
Intraoperative radiographic examination of breast specimens is commonly performed to confirm excision of image-detected breast lesions, but it is not reliable for assessing margin status. A more accurate metho...
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Article
Open AccessOptical endomicroscopy and the road to real-time, in vivo pathology: present and future
Epithelial cancers account for substantial mortality and are an important public health concern. With the need for earlier detection and treatment of these malignancies, the ability to accurately detect precan...
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Article
Imaging the subcellular structure of human coronary atherosclerosis using micro–optical coherence tomography
Progress in understanding coronary artery disease has been hampered by the inability of current approaches to interrogate the human coronary wall at cellular-level resolution. Here, Liu and colleagues introduc...
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Article
Open AccessColor standardization and optimization in Whole Slide Imaging
Standardization and validation of the color displayed by digital slides is an important aspect of digital pathology implementation. While the most common reason for color variation is the variance in the proto...
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Article
Open AccessLocalization of Eosinophilic Esophagitis from H&E stained images using multispectral imaging
This study is an initial investigation on the capability of multispectral imaging to capture subtle spectral information that would enable the automatic delineation between the eosinophilic esophagitis and oth...
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Open AccessNetwork for whole slide imaging (WSI) clinical usage
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Open AccessThe importance of optical optimization in whole slide imaging (WSI) and digital pathology imaging
In the last 10 years, whole slide imaging (WSI) has seen impressive progress not only in image quality and scanning speed but also in the variety of systems available to pathologists. However, we have noticed ...
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Article
Open AccessA relationship between slide quality and image quality in whole slide imaging (WSI)
This study examined the effect of tissue section thickness and consistency – parameters outside the direct control of the imaging devices themselves – on WSI capture speed and image quality. Preliminary data i...
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Article
Open AccessHistology, imaging and new diagnostic work-flows in pathology
Since their introduction in 1999, fully automated, high speed, high-resolution whole slide imaging devices have become increasing more reliable, fast and capable. While by no means perfect, these devices have ...
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Article
Open AccessPrimary histologic diagnosis using automated whole slide imaging: a validation study
Only prototypes 5 years ago, high-speed, automated whole slide imaging (WSI) systems (also called digital slide systems, virtual microscopes or wide field imagers) are becoming increasingly capable and robust....
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Article
Color Correction of Pathological Images Based on Dye Amount Quantification
Pathological images are color images of stained tissue slides, the color of which varies depending on staining conditions. For reliable diagnosis, the color variation must be corrected in these images. This pa...
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Article
Digital Staining of Unstained Pathological Tissue Samples through Spectral Transmittance Classification
Histological structures of a pathological tissue sample convey information relevant to the diagnosis of the disease that might have afficted the person. To reveal the morphology of these structures clearly, pa...
