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Article
Open AccessA novel Escherichia coli cell–based bioreporter for quantification of salicylic acid in cosmetics
Transcription factor–based bioreporters have been extensively studied for monitoring and detecting environmental toxicants. In Escherichia coli, the multiple antibiotic resistance regulator (MarR) induces transcr...
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Article
Open AccessDerivation of pb(II)-sensing Escherichia coli cell-based biosensors from arsenic responsive genetic systems
Heavy metal-responsive operons were used for the generation of Escherichia coli cell-based biosensors. The selectivity and specificity of the biosensors were determined based on the interaction between heavy meta...
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Article
Antimony sensing whole-cell bioreporters derived from ArsR genetic engineering
Despite the known hazardous effects of antimony (Sb) on human health, Sb monitoring biosensors have not been as actively investigated as arsenic (As) biosensors. Whole-cell bioreporters (WCBs) employing an ars...
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Article
Specific heavy metal/metalloid sensors: current state and perspectives
Heavy metal(loid)s play pivotal roles in regulating physiological and developmental aspects in living organisms depending on their concentration. For example, a trace amount of heavy metal(loid)s is essential ...
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Article
Heavy metal(loid) biosensor based on split-enhanced green fluorescent protein: development and characterization
Heavy metal(loid)s such as Cd and Hg adversely affect human health and are therefore strictly regulated and monitored; however, their quantitation in the environment is usually performed by expensive and time-...
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Article
Modulating the sensing properties of Escherichia coli-based bioreporters for cadmium and mercury
Despite the large number of bioreporters developed to date, the ability to detect heavy metal(loid)s with bioreporters has thus far been limited owing to the lack of appropriate genetic systems. We here presen...
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Article
Enhancing the copper-sensing capability of Escherichia coli-based whole-cell bioreporters by genetic engineering
Metals are essential to all organisms; accordingly, cells employ numerous genes to maintain metal homeostasis as high levels can be toxic. In the present study, the gene operons responsive to metal(loid)s were...
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Article
Simultaneous detection of bioavailable arsenic and cadmium in contaminated soils using dual-sensing bioreporters
Whole-cell bioreporters (WCBs) have attracted increasing attention during the last few decades because they allow fast determination of bioavailable heavy metals in contaminated sites. Various WCBs to monitor ...