16 Result(s)
within
Youngdae Yoon
English
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Article
Open AccessA novel Escherichia coli cell–based bioreporter for quantification of salicylic acid in cosmetics
Transcription factor–based bioreporters have been extensively studied for monitoring and detecting environmental toxicants. In Escherichia coli, the multiple antibiotic resistance regulator (MarR) induces transcr...
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Article
Open AccessProduction of isoquercitrin from quercetin by biotransformation using Bascillus sp. CSQ10 isolated from Camellia sinensis cultivation soils
Microorganisms are widely used to produce biologically active substances owing to their versatile ability to convert inexpensive compounds into physiologically active compounds. In this study, we isolated a mi...
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Article
Open AccessAssessing the effects of accumulated Cd(II) on seed germination and root development of Arabidopsis thaliana
In this study, Arabidopsis thaliana was used as a model system to assess the toxic effects of cadmium on plant development and growth. The germination and growth of A. thaliana was inhibited by Cd(II), and the in...
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Article
Open AccessDerivation of pb(II)-sensing Escherichia coli cell-based biosensors from arsenic responsive genetic systems
Heavy metal-responsive operons were used for the generation of Escherichia coli cell-based biosensors. The selectivity and specificity of the biosensors were determined based on the interaction between heavy meta...
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Article
Open AccessAnticancer activities of cyclohexenone derivatives
We designed 21 ethyl 3,5-diphenyl-2-cyclohexenone-6-carboxylate derivatives to identify compounds exhibiting anticancer activity. To measure the inhibitory effects of the compounds on cancer cell growth, a lon...
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Article
Antimony sensing whole-cell bioreporters derived from ArsR genetic engineering
Despite the known hazardous effects of antimony (Sb) on human health, Sb monitoring biosensors have not been as actively investigated as arsenic (As) biosensors. Whole-cell bioreporters (WCBs) employing an ars...
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Article
Specific heavy metal/metalloid sensors: current state and perspectives
Heavy metal(loid)s play pivotal roles in regulating physiological and developmental aspects in living organisms depending on their concentration. For example, a trace amount of heavy metal(loid)s is essential ...
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Article
Heavy metal(loid) biosensor based on split-enhanced green fluorescent protein: development and characterization
Heavy metal(loid)s such as Cd and Hg adversely affect human health and are therefore strictly regulated and monitored; however, their quantitation in the environment is usually performed by expensive and time-...
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Article
Modulating the sensing properties of Escherichia coli-based bioreporters for cadmium and mercury
Despite the large number of bioreporters developed to date, the ability to detect heavy metal(loid)s with bioreporters has thus far been limited owing to the lack of appropriate genetic systems. We here presen...
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Article
Application of metagenome analysis to characterize the molecular diversity and saxitoxin-producing potentials of a cyanobacterial community: a case study in the North Han River, Korea
A wide variety of cyanobacterial species that inhabit freshwater systems are known to produce diverse toxins and off-flavor compounds during the development of environmentally harmful blooms. However, cyanobac...
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Article
Enhancing the copper-sensing capability of Escherichia coli-based whole-cell bioreporters by genetic engineering
Metals are essential to all organisms; accordingly, cells employ numerous genes to maintain metal homeostasis as high levels can be toxic. In the present study, the gene operons responsive to metal(loid)s were...
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Article
Assessing bioavailability and genotoxicity of heavy metals and metallic nanoparticles simultaneously using dual-sensing Escherichia coli whole-cell bioreporters
Genetically engineered bacterial strains called whole-cell bioreporters (WCBs) generated by fusing the promoter region of stress-responsive genes and reporter genes have been widely used as biosensors to detec...
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Article
Simultaneous detection of bioavailable arsenic and cadmium in contaminated soils using dual-sensing bioreporters
Whole-cell bioreporters (WCBs) have attracted increasing attention during the last few decades because they allow fast determination of bioavailable heavy metals in contaminated sites. Various WCBs to monitor ...
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Article
Arsenic bioavailability in soils before and after soil washing: the use of Escherichia coli whole-cell bioreporters
We investigated the quantification of bioavailable arsenic in contaminated soils and evaluation of soil-washing processes in the aspect of bioavailability using a novel bacterial bioreporter developed in prese...
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Article
Phytotoxicity of arsenic compounds on crop plant seedlings
The effects of inorganic and organic arsenic on the germination and seedling growth of 10 crop plants were investigated to elucidate the relationship between toxicity and the arsenic chemical states. Two types...
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Article
In situ quantitative imaging of cellular lipids using molecular sensors
Membrane lipids are dynamic molecules that play important roles in cell signalling and regulation, but an in situ imaging method for quantitatively tracking lipids in living cells is lacking at present. Here, we ...
