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Article
EPS-Producing Microorganisms from Louisiana’s Crusher Juice and the Effect of Processing Conditions on EPS Production
During microbial deterioration of sugarcane, a variety of extracellular polysaccharides (EPS, e.g., dextran, fructans) and other impurities (e.g., organic acids, sugar alcohols, alcohols) are produced. The mic...
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Article
Open AccessFunctional and modular analyses of diverse endoglucanases from Ruminococcus albus 8, a specialist plant cell wall degrading bacterium
Ruminococcus albus 8 is a specialist plant cell wall degrading ruminal bacterium capable of utilizing hemicellulose and cellulose. Cellulose degradation requires a suite of enzymes including endoglucanases, exogl...
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Article
Production of butanol and isopropanol with an immobilized Clostridium
Clostridium beijerinckii optinoii is a Clostridium species that produces butanol, isopropanol and small amounts of ethanol. This study compared the performances of batch and continuous im...
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Article
Glucooligosaccharide production by Leuconostoc mesenteroides fermentation with efficient pH control, using a calcium hydroxide-sucrose solution
95.3% of the sucrose in a feed batch fermentation (300 g/L) was hydrolyzed by Leuconostoc mesenteroides subp. mesenteroides NRRL B-23188 glucansucrase. Further, the glucose of sucrose formed glucooligosaccharides...
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Article
Enhanced production of butanol and isopropanol from sugarcane molasses using Clostridium beijerinckii optinoii
Sugarcane molasses was studied as a substrate for butanol and isopropanol fermentation by Clostridium beijerinckii optinoii in 10 L batch fermentations. Using 3% glucose as a carbon source, the solvent concentrat...
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Article
Lime application for the efficient production of nutraceutical glucooligosaccharides from Leuconostoc mesenteroides NRRL B-742 (ATCC13146)
We have previously demonstrated the production of glucooligosaccharides via a fermentation of sucrose with Leuconostoc mesenteroides NRRL B-742 using sodium hydroxide (NaOH) to control the pH. Because NaOH is exp...
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Article
Inhibitory effects of epigallocatechin gallate and its glucoside on the human intestinal maltase inhibition
Human intestinal maltase (HMA) is an α-glucosidase responsible for the hydrolysis of α-1,4-linkages from the non-reducing end of malto-oligosaccharides. HMA has become an important target in the treatment of t...
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Article
Hydrolytic and phosphorolytic metabolism of cellobiose by the marine aerobic bacterium Saccharophagus degradans 2-40T
Saccharophagus degradans 2-40 is a marine gamma proteobacterium that can produce polyhydroxyalkanoates from lignocellulosic biomass using a complex cellulolytic system. This bacterium has...
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Article
Effects of Epigallocatechin gallate glucoside on antitumor activities in human laryngeal epidermoid carcinoma Hep2
(−)-Epigallocatechin-3-gallate (EGCG) has been reported to inhibit cellular proliferation and induce apoptosis in a range of cancer cells. This study examined the cytotoxicity of EGCG glucoside on human laryng...
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Article
Cloning and expression of the sucrose phosphorylase gene from Leuconostoc mesenteroides in Escherichia coli
The gene encoding sucrose phosphorylase (742sp) in Leuconostoc mesenteroides NRRL B-742 was cloned and expressed in Escherichia coli. The nucleotide sequence of the transformed 742sp comprised an ORF of 1,458 bp ...
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Article
Enzymatic synthesis and characterization of arbutin glucosides using glucansucrase from Leuconostoc mesenteroides B-1299CB
Two arbutin glucosides were synthesized via the acceptor reaction of a glucansucrase from Leuconostoc mesenteroides B-1299CB with arbutin and sucrose. The glucosides were purified by Bio-gel P-2 column chromatogr...
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Article
Characteristics of intermetallic NdNi5 as an unsupported catalyst in thiophene hydrodesulfurization
A systematic research is carried out on intermetallic NdNi5 for the hydrodesulfurization (HDS) of thiophene. The effect of calcination and presulfidation of intermetallic NdNi5 on thiophene HDS was examined and p...
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Article
Thiophene hydrodesulfurization using unsupported nickel-neodymium bimetal catalysts
The hydrodesulfurization of thiophene was studied over unsupported nickel-neodymium bimetallic catalysts. The physical and chemical changes of bimetals were observed after calcination and presulfidation, respe...
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Article
Sorption selectivity of NaY- and KY- zeolites for xylene and toluene
The selective adsorption capacities of NaY- and KY-zeolites for xylene isonners and toluene were investigated by measuring the rate of unidirectional diffusion in a batch adsorber and the breakthrough behavior...