18 Result(s)
within
Yoshihiro Shiraiwa
Plant Sciences
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Article
A novel cell lysis system induced by phosphate deficiency in the cyanobacterium Synechocystis sp. PCC 6803
In the cultivation of microalgae for the production of useful compounds, cell disruption to extract the products of interest is a bottleneck process. To establish a cost-effective method to recover these cellu...
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Article
Changes in the accumulation of alkenones and lipids under nitrogen limitation and its relation to other energy storage metabolites in the haptophyte alga Emiliania huxleyi CCMP 2090
Alkenones are long-chain methyl/ethyl ketones (mainly in length of C37-C39) with two to four trans-unsaturated bonds produced by several kinds of marine haptophytes such as Emiliania huxleyi (coccolithophore). Th...
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Article
Development of engineered sensor perceiving gaseous toluene signal in the cyanobacterium Synechocystis sp. PCC 6803
Microalgae have been used to produce useful compounds. In many cases in which target compounds are produced from CO2 via photosynthesis, there is a trade-off between the proliferation of cells and the production ...
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Article
Open AccessDifference in physiological responses of growth, photosynthesis and calcification of the coccolithophore Emiliania huxleyi to acidification by acid and CO2 enrichment
Ocean acidification, one of the great global environmental issues at present, is expected to result in serious damage on marine calcareous organisms such as corals and calcifying algae, which potentially relea...
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Article
Open AccessGlobal searches for microalgae and aquatic plants that can eliminate radioactive cesium, iodine and strontium from the radio-polluted aquatic environment: a bioremediation strategy
The Fukushima 1 Nuclear Power Plant accident in March 2011 released an enormously high level of radionuclides into the environment, a total estimation of 6.3 × 1017 Bq represented by mainly radioactive Cs, Sr, an...
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Chapter and Conference Paper
Novel Conversion of Chl a into Chl d Catalyzed by Grated Vegetables
Novel conversion of Chl a to Chl d was observed when Chl a was incubated in aqueous acetone with grated papaya(skin), Japanese radish(root), wasabi(rhizome), turnip(root), broccoli(stalk), white radish(leaf), cab...
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Chapter and Conference Paper
Chlorophyll d Production in Crushed Algae in Aqueous Acetone
We for the first time report on the Chl a to Chl d conversion in crushed algae, Synechocystis sp. PCC6803, Emiliania huxleyi, Gephyrocapsa oceanica, Isochrysis galbana, Helladosphaera cornifera, Pleurochrysis car...
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Chapter and Conference Paper
Conversion of Chl a into Chl d by Horseradish Peroxidase
We report for the first time on the Chl a into Chl d conversion with horseradish peroxidase in aqueous acetone at room temperature in the dark, both in the presence and absence of hydrogen peroxide, H2O2. After 4...
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Chapter and Conference Paper
Nonenzymatic Formation of Chl d from Chl a with Hydrogen Peroxide
Nonenzymatic conversion of Chl a to Chl d was performed for the first time by dilute hydrogen peroxide (H2O2) in aqueous acetone (acetone/dilute H2O2 = 10/1, v/v) at 303 K in the dark. The conversion was observed...
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Article
Regulation of the expression of H43/Fea1 by multi-signals
The composition of extracellular proteins is known to be drastically changed in the unicellular green alga Chlamydomonas reinhardtii when the cells are transferred from ambient CO2 to elev...
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Chapter and Conference Paper
Redox Potential of Chlorophyll d
Chl d is a major pigment in a novel oxygenic prokaryote Acaryochloris marina and functions as the primary electron donor P740 in PS I. The midpoint potential of P740 was reported to be +335 mV, which is significa...
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Chapter and Conference Paper
Conversion of Chl a into Chl d by Heat-Treated Papain
In 1943, Chl d was first reported as a minor pigment in several red macroalgae. In 1996, a novel cyanobacterium, Acaryochloris marina, was isolated from colonial ascidians, and A. marina was found to contain Chl
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Chapter and Conference Paper
Succession of Co-factors in Photosystem I
The succession of co-factors in PS I-type reaction centers (RCs) from anoxygenic photosynthesis into oxygenic photosynthesis was considered from the viewpoint of the molecular\ structures. In the PS I-type RCs...
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Article
Minor but key chlorophylls in Photosystem II
A ‘metal-free’ chlorophyll (Chl) a, pheophytin (Phe) a, functions as the primary electron acceptor in PS II. On the basis of Phe a/PS II = 2, Phe a content is postulated as an index for estimation of the stoichi...
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Article
Historical perspective on microalgal and cyanobacterial acclimation to low- and extremely high-CO2 conditions
Reports in the 1970s from several laboratories revealed that the affinity of photosynthetic machinery for dissolved inorganic carbon (DIC) was greatly increased when unicellular green microalgae were transferr...
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Article
A renewable energy source — hydrocarbon gases resulting from pyrolysis of the marine nanoplanktonic alga Emiliania huxleyi
The marine coccolithophore, Emiliania huxleyi, grown in the laboratory was subjected to vacuum pyrolysis at various temperatures from 100 to 500 °C. The highest yield of pyrolytic gases (183 mL g−1 dry cells) was...
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Article
Purification, characterization and cDNA cloning of soluble carbonic anhydrase from Chlorella sorokiniana grown under ordinary air
Soluble carbonic anhydrase (CA, EC 4.2.1.1) inducible by low levels of CO2 was purified from the unicellular green alga Chlorella sorokiniana grown at alkaline pH. The purified CA had a specific activity of 2,30...
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Article
Effect of external CO2 concentrations on protein synthesis in the green algae Scenedesmus obliquus (Turp.) Kütz and Chlorella vulgaris (Kosikov)
Unicellular algae grown under low-CO2 conditions (0.03% CO2) have developed a means of concentrating CO2 at the site of ribulose-1,5-bisphosphate carboxylase/oxygenase. Cells with the CO2-concentrating mechanism ...
