21 Result(s)

Article

Selective inhibition effects on cancer cells and bacteria of Ni–Ti–O nanoporous layers grown on biomedical NiTi alloy by anodization

Stents made of nearly equiatomic NiTi alloy are used to treat malignant obstruction caused by cancer, but prevention of re-obstruction after surgery is still a challenge because the bare stents possess poor an...

Yan-Lian Liu, Yong-Hua Sun, Ya Zhao, Chun-Lin Li, Fei-Long Zhao… in Rare Metals (2022)

Article

Improving exposure of anodically ordered Ni–Ti–O and corrosion resistance and biological properties of NiTi alloys by substrate electropolishing

Although Ni–Ti–O nanopores (NPs) can be fabricated by anodization of mechanically polished NiTi alloys, the top disordered layer is difficult to remove thus hindering the functionality of the Ni–Ti–O NPs. In t...

Yong-Hua Sun, Ya Zhao, Yu-Yu Zhao, You-Jie Rong, Run-Hua Yao, **ao-Hong Yao… in Rare Metals (2021)

Article

Antibacterial ability and cytocompatibility of Cu-incorporated Ni–Ti–O nanopores on NiTi alloy

Nearly equiatomic nickel–titanium (NiTi) alloy is an ideal implant biomaterial because of its shape memory effect, superelasticity, low elastic modulus as well as other desirable properties. However, it is pro...

Jia-Ming Zhang, Yong-Hua Sun, Ya Zhao, Yan-Lian Liu, **ao-Hong Yao, Bin Tang… in Rare Metals (2019)

Article

Deficiency of lrp4 in zebrafish and human LRP4 mutation induce aberrant activation of Jagged–Notch signaling in fin and limb development

Low-density lipoprotein receptor-related protein 4 (LRP4) is a multi-functional protein implicated in bone, kidney and neurological diseases including Cenani-Lenz syndactyly (CLS), sclerosteosis, osteoporosis,...

**g Tian, **hui Shao, Cong Liu, Hsin-Yu Hou… in Cellular and Molecular Life Sciences (2019)

Article

Transcriptional Activity and DNA Methylation Dynamics of the Gal4/UAS System in Zebrafish

The Gal4/upstream activating sequence (UAS) system is a powerful genetic tool for the temporal and spatial expression of target genes. In this study, the dynamic activity of the Gal4/UAS system was monitored i...

Shao-Chen Pang, Hou-Peng Wang, Zuo-Yan Zhu, Yong-Hua Sun in Marine Biotechnology (2015)

Article

Tissue-specific derepression of TCF/LEF controls the activity of the Wnt/β-catenin pathway

Upon stimulation by Wnt ligands, the canonical Wnt/β-catenin signalling pathway results in the stabilization of β-catenin and its translocation into the nucleus to form transcriptionally active complexes with ...

Fu-I Lu, Yong-Hua Sun, Chang-Yong Wei, Christine Thisse… in Nature Communications (2014)

Article

Double Transgenesis of Humanized fat1 and fat2 Genes Promotes Omega-3 Polyunsaturated Fatty Acids Synthesis in a Zebrafish Model

Omega-3 long-chain polyunsaturated fatty acid (n-3 LC-PUFA), especially eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), are essential nutrients for human health. However, vertebrates, including humans...

Shao-Chen Pang, Hou-Peng Wang, Kuo-Yu Li, Zuo-Yan Zhu, **g X. Kang… in Marine Biotechnology (2014)

Article

Targeted Expression in Zebrafish Primordial Germ Cells by Cre/loxP and Gal4/UAS Systems

In zebrafish and other vertebrates, primordial germ cells (PGCs) are a population of embryonic cells that give rise to sperm and eggs in adults. Any type of genetically manipulated lines have to be originated ...

Feng **ong, Zhi-Qiang Wei, Zuo-Yan Zhu, Yong-Hua Sun in Marine Biotechnology (2013)

Article

Activation of GH signaling and GH-independent stimulation of growth in zebrafish by introduction of a constitutively activated GHR construct

Growth hormone (GH) gene transfer can markedly increase growth in transgenic fish. In the present study we have developed a transcriptional assay to evaluate GH-signal activation (GHSA) in zebrafish embryos. By a...

A. S. Ishtiaq Ahmed, Feng **ong, Shao-Chen Pang, Mu-Dan He… in Transgenic Research (2011)

Article

Identification of a novel gene K23 over-expressed in fish cross-subfamily cloned embryos

A novel gene—K23, differentially expressed in cross-subfamily cloned embryos, was isolated by RACE-PCR technique. It had 2580 base pairs (bp) in length, with a 1,425 bp open reading frame (ORF) encoding a puta...

De-Sheng Pei, Yong-Hua Sun, Zuo-Yan Zhu in Molecular Biology Reports (2009)

Article

Inhibition of no tail (ntl) gene expression in zebrafish by external guide sequence (EGS) technique

External guide sequence (EGS) technique, a branch of ribozyme strategy, can be enticed to cleave the target mRNA by forming a tRNA-like structure. In the present study, no tail gene (ntl), a key gene participatin...

De-Sheng Pei, Yong-Hua Sun, Yong Long, Zuo-Yan Zhu in Molecular Biology Reports (2008)

Article

Construction of cytoplasmic molecular markers distinguishing Danio rerio from Gobiocypris rarus at high identity domains based on MP-PCR strategy and Sybr Green I detection

To distinguish the cytoplasm of Danio rerio from that of Gobiocypris rarus, we cloned G. rarus COXI and constructed cytoplasmic molecular markers at the high identity domains of COXI by mutated primer PCR (MP-PCR...

De Sheng Pei, Yong Hua Sun, Zuo Yan Zhu in Molecular Biology Reports (2008)

Article

Knock down of gfp and no tail expression in zebrafish embryo by in vivo-transcribed short hairpin RNA with T7 plasmid system

A short-hairpin RNA (shRNA) expression system, based on T7 RNA polymerase (T7RP) directed transcription machinery, has been developed and used to generate a knock down effect in zebrafish embryos by targeting gre...

Na Wang, Yong-Hua Sun, **g Liu, Gang Wu, Jian-Guo Su… in Journal of Biomedical Science (2007)

Article

Molecular characterization of common carp (Cyprinus carpio) Sonic Hedgehog and discovery of its maternal expression

Sonic hedgehog (Shh), one of important homologous members of the hedgehog (Hh) family in vertebrates, encodes a signaling molecule that is involved in short- or long-range patterning processes du...

Na Wang, Yong-Hua Sun, **g Liu, Ya-** Wang… in Development Genes and Evolution (2007)

Article

Cloning, characterization and promoter analysis of common carphairy/Enhancer-of-split-related gene,her6

Some members ofhairy/Enhancer-of-split-related gene (HES) family have important effects on axial mesoderm segmentation and the establishment and maintenance of the somite fringe. In fishes, theher6 gene, a member...

**g Liu, Yong-Hua Sun, Na Wang, Ya-** Wang, Zuo-Yan Zhu in Journal of Genetics (2006)

Article

Upstream Regulatory Region of Zebrafish lunatic fringe: Isolation and Promoter Analysis

Lunatic fringe (Lfng), one modulator of Notch signaling, plays an essential part in demarcation of tissues boundaries during animal early development, especially somitogenesis. To characterize the promoter of ...

**g Liu, Yong-Hua Sun, Na Wang, Ya-** Wang, Zuo-Yan Zhu in Marine Biotechnology (2006)

Article

Sequences of Transgene Insertion Sites in Transgenic F4 Common Carp

Bo Wu, Yong-Hua Sun, Ya-** Wang, Yan-Wu Wang, Zuo-Yan Zhu in Transgenic Research (2004)