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Article
Open AccessImportance of pre-mRNA splicing and its study tools in plants
Alternative splicing (AS) significantly enriches the diversity of transcriptomes and proteomes, playing a pivotal role in the physiology and development of eukaryotic organisms. With the continuous advancement...
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Article
First report of Pestalotiopsis neolitseae causing leaf blight on Photinia × fraseri in China
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Article
Plant serine/arginine-rich proteins: versatile players in RNA processing
Serine/arginine-rich (SR) proteins participate in RNA processing by interacting with precursor mRNAs or other splicing factors to maintain plant growth and stress responses.
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Protocol
In Situ Observation of Abscisic Acid Distribution in Major Crop Species by Immunofluorescence Labeling
Immunofluorescent approach uses fluorophore-conjugated antibodies to target molecules of interest for the observation of their distribution and microenvironment at cellular or tissue level. In connection with ...
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Article
Phylogeny and conservation of plant U2A/U2A’, a core splicing component in U2 spliceosomal complex
This study systematically identifies 112 U2A genes from 80 plant species by combinatory bioinformatics analysis, which is important for understanding their phylogenetic history, expression profiles and for pre...
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Article
Open AccessPhylogenetic comparison and splice site conservation of eukaryotic U1 snRNP-specific U1-70K gene family
Eukaryotic cells can expand their coding ability by using their splicing machinery, spliceosome, to process precursor mRNA (pre-mRNA) into mature messenger RNA. The mega-macromolecular spliceosome contains mul...
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Article
Open AccessSWATH-MS-facilitated proteomic profiling of fruit skin between Fuji apple and a red skin bud sport mutant
Apple is one of the most popular fruit crops world-wide and its skin color is an important quality consideration essential for commercial value. However, the strategy on genetic breeding for red skin apple and...
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Article
Comparative metabolite profiling of two switchgrass ecotypes reveals differences in drought stress responses and rhizosheath weight
Rhizosheath comprises soil that adheres firmly to roots. In this study, two ecotypes of switchgrass with different rhizosheath sizes after drought stress were analyzed which showed metabolic differences under ...
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Article
Genome-wide identification and functional analysis of the splicing component SYF2/NTC31/p29 across different plant species
This study systematically identifies plant SYF2/NTC31/p29 genes from 62 plant species by a combinatory bioinformatics approach, revealing the importance of this gene family in phylogenetics, duplication, trans...
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Article
Open AccessComparative performance of the BGISEQ-500 and Illumina HiSeq4000 sequencing platforms for transcriptome analysis in plants
The next-generation sequencing (NGS) technology has greatly facilitated genomic and transcriptomic studies, contributing significantly in expanding the current knowledge on genome and transcriptome. However, t...
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Article
Abscisic acid is required for somatic embryo initiation through mediating spatial auxin response in Arabidopsis
Abscisic acid (ABA) regulates many aspects of plant development, including somatic embryo (SE) initiation. However, mechanisms of ABA functions on SE initiation have remained to be investigated. In this study,...
